Telomerase activity and lymphovascular invasion in breast cancer

Mokbel, KM; Parris, C.N.; Ghilchik, M.W.; Amerasinghe, CN; Newbold, RF

doi:10.1053/ejso.1999.0736

Cited by 40 publications

(25 citation statements)

References 19 publications

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“…However telomerase activity was absent in 19-32% of less advanced breast cancer specimens [79]. Our group reported that telomerase activity was associated with high tumour grade, tumour type, nodal metastasis, high cellular proliferation [80], large tumour size, and lymphovascular invasion in invasive breast cancer [20,[80][81][82][83]. However, telomerase activity seemed, in these studies, to be independent of hormonal receptor status and p53 expression [82].…”

Section: Discussionmentioning

confidence: 99%

The expression of gene transcripts of telomere-associated genes in human breast cancer: correlation with clinico-pathological parameters and clinical outcome

Salhab

Jiang

Newbold

et al. 2007

Breast Cancer Res Treat

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Genes encoding telomere-associated proteins display different patterns of mRNA expression in human breast cancer, and in normal breast tissue, suggesting different and sometimes opposing roles in mammary carcinogenesis. hTERT, hTR, TANK1, EST1 and TEP1 seem to be up-regulated, with hTERT and TEP1 correlating with clinical outcome. Conversely, TANK2 and POT1 transcription levels demonstrate a compelling trend to be lower in malignant tissues and lower still in those patients who develop recurrent disease suggesting that TANK2 and POT1 may act as tumour suppressor genes possibly by negatively regulating telomerase activity.

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Section: Discussionmentioning

confidence: 99%

The expression of gene transcripts of telomere-associated genes in human breast cancer: correlation with clinico-pathological parameters and clinical outcome

Salhab

Jiang

Newbold

et al. 2007

Breast Cancer Res Treat

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“…39,43 Various studies of human breast cancer focused on the importance of this enzyme as a diagnostic marker for malignant behavior because the enzyme activity was reported to be correlated to tumor malignancy, 36,44,49 high-stage malignant compared with low-stage, 3,23 in those cases with lymphatic or lymph node invasion, or both, in comparison with those without such an involvement. 34,37 The general idea is that telomerase should not be considered an absolute marker of malignancy; this is because its activity has been found in benign tumors as well, even though high-grade malignant tumors are more often detected with higher levels of expression. Similar conclusions can be drawn for animal tumors as well.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of Telomerase in Canine Mammary Tissues by Immunohistochemical Analysis and a Polymerase Chain Reaction-Based Enzyme-Linked Immunosorbent Assay

2006

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Abstract. The enzyme telomerase is considered a potential marker for neoplastic tissue and is used as a diagnostic and prognostic tool in clinical medicine and therapeutics. For this reason, the possible role of telomerase activation in the process of malignant transformation is currently the subject of intense research efforts. The focus of the study reported here was to detect telomerase in 37 canine mammary samples, by comparing two methods: immunohistochemical (IHC) analysis for detecting the catalytic subunit of the enzyme, telomerase reverse transcriptase (TERT), and the telomeric repeat amplification protocol-enzymelinked immunosorbent assay (TRAP-ELISA), a polymerase chain reaction (PCR)-based technique that uses a colorimetric detection method. Using the TRAP-ELISA, samples were considered positive when they yielded a difference of at least 0.2 absorbance units between the readings at 450 nm versus 690 nm wavelength. On the basis of this criterion, 18 negative and 19 positive cases were obtained. Specific immunohistochemical staining was observed mainly in the nucleoli, to a lesser extent in the nuclei, and rarely in the cytoplasm of epithelial cells. A sample was considered positive when at least 10% of the epithelial cells had specific staining. The Pearson correlation between the TRAP-ELISA and IHC results was significant only when IHC nucleolar (r 5 0.53, P , 0.01) or nuclear (r 5 0.36, P , 0.05) staining or their combination (r 5 0.58, P , 0.01) was considered. Thus, IHC staining of nucleoli and nuclei can be considered as an alternative method to the TRAP-ELISA. The detection of telomerase in normal mammary gland and fibrocystic mastopathy using both methods does not support the idea that telomerase may be used as a specific marker of mammary neoplasia in dogs.

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“…Hoos et al found a significant correlation between telomerase activity and tumor size, lymph node status, and stage [16]. A significant association between telomerase-positive infiltrating breast carcinomas and lymphovascular invasion, a fundamental step in breast cancer metastasis and a predictor of survival, has also been observed, making telomerase a useful prognostic marker [17]. Clark et al reported, in a prognostic study involving 398 patients with lymph node-positive breast cancer, that increased telomerase activity was associated with decreased disease-free survival [18].…”

Section: Telomerase Activity and Prognosis In Breast Cancermentioning

confidence: 99%

Telomerase and breast cancer

2001

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Current therapies for breast cancer include treatments that are toxic and often result in drug resistance. Telomerase, a cellular reverse transcriptase that maintains the ends of chromosomes (telomeres), is activated in the vast majority of breast cancers (over 90% of breast carcinomas) but not in normal adjacent tissues. Telomerase is thus an attractive target for both diagnosis and therapy because of its distinct pattern of expression. We address the use of telomerase in the diagnostics of breast pathology, as well as the use of telomerase inhibitors in the treatment and prevention of breast cancer.

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Telomerase activity and lymphovascular invasion in breast cancer

Cited by 40 publications

References 19 publications

The expression of gene transcripts of telomere-associated genes in human breast cancer: correlation with clinico-pathological parameters and clinical outcome

The expression of gene transcripts of telomere-associated genes in human breast cancer: correlation with clinico-pathological parameters and clinical outcome

Evaluation of Telomerase in Canine Mammary Tissues by Immunohistochemical Analysis and a Polymerase Chain Reaction-Based Enzyme-Linked Immunosorbent Assay

Telomerase and breast cancer

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