Telomerase activity associated with progression of cervical lesions in a group of Colombian patients

Castro-Duque, Andrés Felipe; Loango-Chamorro, Nelsy; Ruiz-Hoyos, Bayron Manuel; Landázuri, Patricia

doi:10.1590/so100-720320150005462

Cited by 6 publications

(12 citation statements)

References 25 publications

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“…TA was detected in 76% of the cases studied, and in 21.1% of the controls. These results are in agreement with previous studies which have detected TA in cancer and precancerous lesions while showing undetectable or low TA levels in normal tissue ( 12 , 13 ). Similar results were observed for the detection of HPV DNA.…”

Section: Discussionsupporting

confidence: 93%

“…Identifying molecular markers that will permit diagnosis and prognosis of women who have cervical lesions as well as predicting which precursor lesions are likely to become cancerous will lead to improvements in cervical cancer prevention. Several studies on women with normal cytology and/or different grades of cervical lesions have shown increased TA in higher grade lesions ( 10 – 13 ). Some studies have also shown that infections with HPV 16 and 18 are associated with increased TA ( 14 , 15 ).…”

Section: Introductionmentioning

confidence: 99%

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Telomerase activity in cervical scrapes of women with high‑grade cervical disease: A nested case-control study

et al. 2017

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Epidemiological information on telomerase activity (TA) and development of cervical lesions is scarce. A nested case-control study was carried out within a cohort of Colombian women tested for Human Papillomavirus (HPV). Measurement of TA was done in cervical scrapes of 25 women who developed High Grade Squamous Intraepithelial Lesion (HGSIL) during the first 6 years of follow-up and was compared with that of 104 control women who maintained normal cytology during the entire follow-up. TA was measured by a telomerase repeat amplification protocol-ELISA. TA and HPV infections were significantly more frequent in cases than in controls. Likewise, 68% of the cases were positive for both TA and HPV compared with only 7.7% of the controls (P<0.0001). Factors independently associated with increased odds of HGSIL included TA, high risk HPV (hrHPV) infections and multiple parities. When restricted to hrHPV positive women, TA was strongly associated with increased odds of HGSIL (adjusted odds ratio=37.94, 95% confidence interval, 1.64–678.1). In addition to an infection with hrHPV, TA appears to be a significant cofactor for HGSIL.

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Section: Discussionsupporting

confidence: 93%

Section: Introductionmentioning

confidence: 99%

Telomerase activity in cervical scrapes of women with high‑grade cervical disease: A nested case-control study

et al. 2017

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“…Duque et al, had similar results by TRAP assay regarding the telomerase activity in range from 75.4 % in LSIL to 100% in HSIL, ASC-H and cervical cancer [37].…”

Section: Saretzki Et Al Found No Correlation Between the Telomerase mentioning

confidence: 66%

“…[33,34]. Published studies on the subject reveal that hTERT expression and telomerase activity was reported in 91.3%, 96.5% and 100% of cervical cancer samples [35][36][37]. Increased hTERT expression was also seen in 55.0% of CIN II and 82.1 % of CIN III diagnosed [38].…”

Section: Discussionmentioning

confidence: 91%

Correlation of hTERT Expression with Cervical Cytological Abnormalities and Human Papillomavirus Infection

Zejnullahu

Josifovska

Vukovik

et al. 2017

Prilozi

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Telomerase Reverse Transcriptase (TERT) is the main catalytic sub-unit of telomerase, a reverse transcriptase enzyme. Telomerase expression is regulated at many levels, with numerous studies suggesting that up-regulation of human TERT gene (hTERT) at transcriptional level results in immortal cell phenotype associated with cancer. The aim of this study is to determine the correlation between hTERT expression and different cervical precursor lesions, as well as with cervical cancer in patients with confirmed Human papillomavirus (HPV) infection. The study included molecular analyzes on cervical samples from 214 women and matched Papanicolaou (Pap) test results. HPV detection and genotyping was performed by polymerase chain reaction (PCR) and genotyping. Quantitative real-time PCR (qRT-PCR) was performed using TaqMan probes and were calculated relative to the reference gene. Results showed significantly increased hTERT mRNA expression levels in high-grade and low-grade lesions compared to normal control samples (p<0.01) associated with 6.31 fold higher risk for developing ASC-US and 9.20 for LSIL. Strong correlation between HPV infection and hTERT expression in the high-grade lesions and cervical cancer was also observed. hTERT relative expression values showed 98% specificity and 100 % sensitivity as indicator of cervical lesions particularly for the ACS-H, HSIL and cervical cancer. In conclusion, hTERT expression correlate with the cytological grade of the cervical lesions and HPV infection and has a potential to be used as a diagnostic and prognostic marker.

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“…Telomeres, the terminal structures of chromosomes, are composed of long, repetitive 6-base pair nucleoproteins. Their vital role is to maintain chromosomal integrity and genomic stability by blocking the end-to-end fusion of chromosomes, nuclease degradation, and incomplete replication during cell division [1,2]. In human somatic cells, the average length of telomeres is 10-15 kb, and telomeric DNA is shortened during each cell division by approximately 50-200 base pairs [3,4].…”

Section: Introductionmentioning

confidence: 99%

Prognostic Value of Peripheral Blood Lymphocyte Telomere Length in Gynecologic Malignant Tumors

Shanta

Nakayama

Ishikawa

et al. 2020

Cancers

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Background: Lymphocyte telomere length is strongly correlated with patient prognosis in several malignant tumor types and is thought to be related to tumor immunity. However, this correlation has not been studied in gynecological cancers. We determined the prognostic significance of peripheral blood lymphocyte telomere length in gynecologic cancers. Methods: Telomere length of lymphocytes from patients with gynecological malignant tumors (ovarian cancer (OC), N = 72; cervical cancer (CC), N = 63; endometrial cancer (EC), N = 87) was examined by quantitative reverse-transcription PCR of isolated mononuclear cells. Kaplan–Meier and Cox proportional hazard analyses were used to determine the association between lymphocyte telomere length and clinicopathological factors. Results: The overall survival (OS) and progression-free survival (PFS) of patients were based on the dichotomized lymphocyte telomere length using the median as a threshold (OC: 0.75, CC: 1.94, and EC: 1.09). A short telomere length was significantly correlated with residual tumors (≥1 cm) in OC and with advanced stage (III and IV) of CC. In OC and CC, patients with shorter relative lymphocyte telomere length (RLT) had significantly poorer OS and PFS than patients with longer RLT (p = 0.002, p = 0.003, and p = 0.001, p = 0.001, respectively). However, in EC, RLT was not significantly associated with OS or PFS (p = 0.567 and p = 0.304, log-rank test). Multivariate analysis showed that shorter RLT was a significant independent prognostic factor of PFS and OS for OC (p = 0.03 and p = 0.04, respectively) and CC (p = 0.02 and p = 0.03, respectively). Conclusions: Patients with OC and CC with shorter lymphocyte telomeres have significantly reduced survival; therefore, the peripheral blood lymphocyte telomere length is a prognostic biomarker in OC and CC.

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Telomerase activity associated with progression of cervical lesions in a group of Colombian patients

Cited by 6 publications

References 25 publications

Telomerase activity in cervical scrapes of women with high‑grade cervical disease: A nested case-control study

Telomerase activity in cervical scrapes of women with high‑grade cervical disease: A nested case-control study

Correlation of hTERT Expression with Cervical Cytological Abnormalities and Human Papillomavirus Infection

Prognostic Value of Peripheral Blood Lymphocyte Telomere Length in Gynecologic Malignant Tumors

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