Telomerase Activity in Oral Squamous Cell Carcinoma

Curran, Aongus J.; Denis, Kelly St.; Irish, Jonathan C.; Gullane, Patrick; MacMillan, Christina; Kamel‐Reid, Suzanne

doi:10.1001/archotol.124.7.784

Cited by 22 publications

(32 citation statements)

References 20 publications

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“…9 This was not a feature of this series of laryngeal squamous cell carcinomas, as telomerase levels were not predictive of T stage. Also, no correlation was found between telomerase activity and clinical parameters such as tumor site or histological grade.…”

Section: Discussionmentioning

confidence: 69%

“…This is in keeping with recent literature that has reported telomerase activity in a range of differing human cancers and our findings with oral squamous cell carcinoma. 9,10,12 The presence of the immortalizing enzyme in laryngeal squamous cell carcinoma suggests that it has a role to play in continuous cell proliferation and tumorigenesis. Our findings have added significance in that telomerase is still activated in patients after failure of radiotherapy and therefore may have a role as a biological marker of malignancy.…”

Section: Discussionmentioning

confidence: 99%

“…6,7,9 This suggests that the enzyme is not essential for the maintenance of a malignant phenotype in all cases. Alternatively, telomerase could have been activated at some stage in the progression of malignancy in these individuals yet was downregulated Table I) and are both positive.…”

Section: Discussionmentioning

confidence: 99%

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Telomerase Activity Is Upregulated in Laryngeal Squamous Cell Carcinoma

et al. 2000

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Objective/Hypothesis: The immortalizing enzyme telomerase has been linked to carcinogenesis and is being targeted as a novel molecular marker. This study investigated telomerase expression in patients with laryngeal squamous cell carcinoma and correlated telomerase activity with conventional prognostic parameters. Study Design: A consecutive series of patients with laryngeal squamous cell carcinoma undergoing surgical salvage for persistent or progressive disease after failed radiation therapy. Methods: Twenty patient samples of laryngeal squamous cell carcinoma and 20 adjacent histologically normal mucosal samples were assayed using the telomeric repeat amplification protocol (TRAP) method for detection of telomerase activity. The leukemic cell line, K562, acted as a positive control and the human fibroblast line, Hs21Fs, as a negative control. A sample was classified as telomerase positive when an RNasesensitive hexameric repeat ladder was observed. Absence of laddering was considered a negative result. Results: Seventeen of 20 (85%) tumor samples and 4 of 20 (20%) adjacent histologically normal samples were telomerase positive. No statistically significant difference was observed when densitometric readings were compared by T category, tumor grade, or site (by ANOVA). Conclusions: Although telomerase activity is present in laryngeal cancer, levels of activation do not correlate with conventional parameters used for prognostication. Our study indicates that the marker may be a useful adjunctive method in the diagnosis of malignancy after radiation failure.

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Section: Discussionmentioning

confidence: 69%

Section: Discussionmentioning

confidence: 99%

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Telomerase Activity Is Upregulated in Laryngeal Squamous Cell Carcinoma

et al. 2000

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“…Activation of telomerase points to an early carcinogenesis in this location (112)(113)(114)(115)(116)(117). High telomerase activity can be demonstrated in the permanent cell lines established from head and neck tumors but also in the immortalized keratinocyte cell lines HaCaT (139).…”

Section: Proof Of Telomerase As a Potential Biomarkermentioning

confidence: 99%

“…In carcinoma-free tumor margin tissue from HNSCC, telomerase activation indicates the occurrence of field cancerization in this tissue and the first steps of carcinogenesis (110,(112)(113)(114)(115)(116)(117). Geyer et al (17), Hong et al (27), Lieberman et al (100), Koch (101), Kelloff et al (102) and Smith and Saba (107) recommended telomerase as a biomarker for assessing chemoprevention in tumors in the head and neck tumors region.…”

Section: Introductionmentioning

confidence: 99%

Model examinatioï¿½n of chemoprevention with retinoids in squamous cell carcinomas of the head and neck region and suitable biomarkers for chemoprevention

Fabricius

Kruse-Boitschenko²,

Schneeweiss³

et al. 2011

Int J Oncol

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Prognostic value of telomerase and DNA ploidy in laryngeal carcinoma

Samny

Halaby

Fiky

et al. 2005

Eur Arch Otorhinolaryngol

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Prognosis of carcinoma of the larynx is currently based on the morphologic analysis of the tumor extent, differentiation grading and type of microscopic invasion. The DNA ploidy status and the cell proliferation activity may give complementary information about the prognosis. Telomerase may be used as a measure of tumor proliferation. The aim of this work was to investigate the probable application of telomerase as a measure of cell immortalization as well as DNA ploidy as a measure of cell proliferation in the prognosis of patients with laryngeal carcinoma. The work was carried out as a prospective observational study that included 35 patients with laryngeal carcinoma who were treated surgically at Ain Shams University Hospitals, Cairo, Egypt. Flowcytometric analysis and biochemical assay of telomerase using human Telomerase Reverse Transcriptase (hTERT) mRNA assay by RT-PCR in resected specimens were done. The proliferative indices in different parts of the tumor were found to be significantly different from each other, with those of tumor edges being the highest. The ploidy status at the edge of the tumor was significantly different between surviving and non-surviving patients, while the tumor core proliferative index was found to be significantly lower in patients without recurrence than those with recurrence. A gradient of telomerase levels was noticed, with levels from tumor cores being the highest followed by tumor edges, resection margins and the lowest levels in lymph nodes. Multivariate analysis showed hTERT levels in tumor edges to significantly affect survival. Both Flowcytometric parameters and telomerase activity may be useful as a prognostic factor for survival in patients with laryngeal carcinoma. Telomerase activity may be used for biologic demarcation for conservational laryngeal surgery, as well as a 'spectral' marker.

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Telomerase Activity in Oral Squamous Cell Carcinoma

Abstract: The finding of telomerase activity in 90% of tumor samples is consistent with the concept of telomerase playing a key role in tumorigenesis. Further study is needed to determine the usefulness of this enzyme as a molecular marker.

Cited by 22 publications

References 20 publications

Telomerase Activity Is Upregulated in Laryngeal Squamous Cell Carcinoma

Telomerase Activity Is Upregulated in Laryngeal Squamous Cell Carcinoma

Model examinatioï¿½n of chemoprevention with retinoids in squamous cell carcinomas of the head and neck region and suitable biomarkers for chemoprevention

Prognostic value of telomerase and DNA ploidy in laryngeal carcinoma

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