Telomere Dynamics in the Diploid and Triploid Rainbow Trout (Oncorhynchus mykiss) Assessed by Q-FISH Analysis

Abstract

Changes of telomere length with age were assessed in diploid and triploid rainbow trout (Oncorhynchus mykiss) females in the cross-sectional study using Q-FISH technique. Triploid trout as sterile do not invest an energy in gametogenesis and continue to grow, whereas fertile diploid individuals suffer from declines in growth and survival during sexual maturation. However, triploid and diploid specimens exhibited similar patterns of telomere dynamics. Telomere length in the embryos, larvae and one-year-old juveniles did not change significantly. In the second year after hatching, subadults exhibited substantially shortened telomeres, while significant increase of the telomere length was reported in the three-year-old adults. On the other hand, correlation between telomere length and body size was observed in the triploid, but not in the diploid rainbow trout. Telomere shortening observed in two-year-old subadults may have been associated with the premature period of the fast growth in rainbow trout. Similar pattern of the telomere dynamics reported in the fertile diploids and sterile triploids indicated processes related to reproduction did not affect telomere dynamics in this species. Unexpected increase of the telomere length reported during the third year of life confirmed that in rainbow trout telomeric DNA shortens and lengthens, depending on the developmental stage.

Telomere length variation does not correspond with the growth disturbances in the rainbow trout (Oncorhynchus mykiss)

“…One-year-old dwarf androgenetic individuals ( n = 7), normally developed androgenetic specimens ( n = 7) and their normally developed siblings from control group ( n = 7) were sampled and examined. The sample size was determined to meet requirements for the statistical analysis and it was based on the previous studies using Q-FISH performed in zebrafish (Anchelin et al 2011 ) and rainbow trout (Panasiak et al 2020 ). All rainbow trout were maintained under the same husbandry and environmental conditions.…”

Patterns of telomere length with age in African mole-rats: New insights from quantitative fluorescence in situ hybridisation (qFISH)

“…in mouse strains: Zijlmans et al, 1997 ), comparable with telomere length measurement method using Southern blotting/in situ hybridisation techniques and in some applications may be more precise than qPCR methods ( Hultdin et al, 1998 ; Gutierrez-Rodrigues et al, 2014 ). Apart from mammals, qFISH has also been employed as a single technique estimator of telomere length in diverse vertebrate taxa such as fish ( Panasiak, Dobosz & Ocalewicz, 2020 ) and reptiles ( Olsson et al, 2020 ). PNA-qFISH clearly identified terminal telomeric repeats in the DMR, MMR and NMR and we report for the first time ITSs in the DMR and tentatively in the NMR ( Figs.…”

Telomere length variation does not correspond with the growth disturbances in the rainbow trout (Oncorhynchus mykiss)

“…One-year-old dwarf androgenetic individuals (n = 7), normally developed androgenetic specimens (n = 7) and their normally developed siblings from control group (n = 7) were sampled and examined. The sample size was determined to meet requirements for the statistical analysis and it was based on the previous studies in sh The sample size was determined to meet requirements for the statistical analysis and it was based on the previous studies using Q-FISH performed in zebra sh (Anchelin et al 2011) and rainbow trout (Panasiak et al 2020). All rainbow trout were maintained under the same husbandry and environmental conditions.…”

“…Microscopic slides with sh cells and DBA/2 lymphoma cells were prepared the day before PNA-FISH experiment and kept at the room temperature. Q-FISH analysis has been performed using the method described in details in (Panasiak et al 2020). Shortly, after the PNA-FISH slides were scanned with the ASI system, HiFISH-SpotScan module (Applied Spectral Imaging, Yokne'am Illit, Israel) with a dedicated 5M CMOS camera connected to a uorescent BX53 Olympus microscope.…”