Telomere length in Agave tequilana Weber plants during the in vitro to ex vitro transition

Rescalvo-Morales, A.; Monja-Mio, Kelly M.; Robert, Manuel L.; Teyer, Lorenzo Felipe Sánchez

doi:10.1007/s11240-018-1499-1

Cited by 8 publications

(8 citation statements)

References 54 publications

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“…At all the timepoints, however, the mature somatic embryos had shorter telomeres than immature zygotic embryos or ETs containing proembryos. Although this difference with ETs was non-significant, it is consistent with the previous observations of telomeres shortening with differentiation or degree of tissue maturity [ 9 , 13 , 14 ].…”

Section: Discussionsupporting

confidence: 92%

“…In the present material, the observed telomere shortening cannot be related to tissue differentiation, since proliferating ETs, also called proembryogenic masses (PEMs) contain two major cell types: Meristematic cells of the embryonal mass and the embryonal tube cells [ 34 ], so the shortening is most probably related to initiation stress. Initiation of other types of tissue culture than SE has resulted on contradictory observations on telomere length: Induction of organogenesis in agave species resulted in telomere lengthening [ 13 ], while no change in telomere length was found in tobacco plants produced by organogenesis via callus phase [ 16 ].…”

Section: Discussionmentioning

confidence: 99%

“…There is, however, only limited information on the effect biotechnological tools, such as tissue culture or cryopreservation on plant telomeres despite the fact that conditions within these techniques per se can be argued to act as stress [ 12 ], followed by a transition to ex vitro conditions known to be stressful to plants too [ 13 ]. Some studies have shown shortening of telomeres in perennial plants, as found in in vitro shoot and callus cultures of deciduous tree silver birch [ 10 ], as well as following ex vitro acclimatization of agave ( Agave tequilana Weber) [ 13 ]. In agave, however, temporary shortening of telomeres was found to be resumed and telomere length maintained thereafter, either based on telomerase activity or alternative mechanisms.…”

Section: Introductionmentioning

confidence: 99%

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Telomere Length in Norway Spruce during Somatic Embryogenesis and Cryopreservation

Aronen

Virta

Varis

2021

Plants

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Telomeres i.e., termini of the eukaryotic chromosomes protect chromosomes during DNA replication. Shortening of telomeres, either due to stress or ageing is related to replicative cellular senescence. There is little information on the effect of biotechnological methods, such as tissue culture via somatic embryogenesis (SE) or cryopreservation on plant telomeres, even if these techniques are widely applied. The aim of the present study was to examine telomeres of Norway spruce (Picea abies (L.) Karst.) during SE initiation, proliferation, embryo maturation, and cryopreservation to reveal potential ageing or stress-related effects that could explain variation observed at SE process. Altogether, 33 genotypes from 25 families were studied. SE initiation containing several stress factors cause telomere shortening in Norway spruce. Following initiation, the telomere length of the embryogenic tissues (ETs) and embryos produced remains unchanged up to one year of culture, with remarkable genotypic variation. Being prolonged in vitro culture can, however, shorten the telomeres and should be avoided. This is achieved by successful cryopreservation treatment preserving telomere length. Somatic embryo production capacity of the ETs was observed to vary a lot not only among the genotypes, but also from one timepoint to another. No connection between embryo production and telomere length was found, so this variation remains unexplained.

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Section: Discussionsupporting

confidence: 92%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Telomere Length in Norway Spruce during Somatic Embryogenesis and Cryopreservation

Aronen

Virta

Varis

2021

Plants

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“…In our group, we carried out studies of active telomerase throughout the vegetative development of the leaves under field conditions 1-6 years of age, and we observed that telomerase activity associated with cellular activities such as cell elongation, division, and differentiation. We also observed high telomerase activity throughout the growth phase, while telomere length is maintained during this phase, indicating that telomerase contributes telomeric maintenance (Rescalvo-Morales et al 2016; De la Torre-Espinosa et al 2020). There have also been studies of the dynamics in telomeric length in different tissues from 1 to 3 years of age, observing that telomeric length is tissue specific with shorter telomeres in differentiated and elongated tissues in tissues with active division (Rescalvo-Morales et al 2019).…”

Section: Introductionsupporting

confidence: 62%

AteqTERT expression and specific tissue activity in a 2-year-old complete plant in Agave tequilana in field conditions

et al. 2020

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Telomerase is a specialized enzyme that attributes to maintaining and lengthening of telomeric length as its primary function. This ribonucleoprotein complex requires two minimum subunits for its in vivo function: the TERT amino acid subunit and TER its ribonucleic subunit. Crystallographic studies have shown that stable interaction between subunits and their domains is essential during the active phase. Plants and humans have shown to be tissue specific and dependent on cell proliferation. In Arabidopsis thaliana (L.) Heynh, the expression of AtTERT was determined to be necessary for activity. The objective of this work is to perform the in silico characterization of TERT in Agave tequilana F.A.C. Weber and evaluate the expression of TERT and the specific tissue activity in 2-year-old plants. To this end, we use the transcriptome library of A. tequilana (Ávila de Dios unpublished data) in TPM and qTRAP. We observe in this work that the consensus sequence shows all the specific domains of the TERT subunit. Additionally, we carried out the in silico prediction of the structural model of AteqTERT. The model presents the characteristic TERT ring of the crystallized models in active state of telomerase, biological annotations were observed, such as heavy metals Mg 2+ , NUC and CH1, and finally we note that, there is no correlation between TERT expression in TPM and telomerase activity in 2-year meristematic tissue.

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“…If the plant material is not vigorous, abnormal stomatal development, absence of epicuticular waxes and few functional roots may occur. An effective micropropagation protocol is essential to achieve high levels of survival (Rescalvo-Morales et al 2019;Monja-Mio et al 2020).…”

Section: Determination Of Viability and Phytosanitary Quality Of In V...mentioning

confidence: 99%

Development of control strategies for bacteria and fungi associated with a micropropagated new cultivar of orange-fleshed sweet potato (Ipomoea batatas cv. Agrosavia–Aurora)

Pérez-Pazos

Rosero

Cardinale

et al. 2023

Hortic. Environ. Biotechnol.

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Vegetative propagation of sweet potato lead to the accumulation of diseases from generation to generation, which represents a threat to both productivity and conservation of genetic resources. In vitro techniques can help to overcome phytosanitary problems by applying plant material cleaning strategies. The objective of this study was to develop in vitro micropropagation strategies for the production of high-quality plant material of an orange-fleshed variety of sweet potato recently released in Colombia. Molecular identification of contaminating microorganisms was performed by sequencing the 16S rRNA gene for bacteria and ITS for fungi. Five disinfection protocols were evaluated, three of which were previously developed for sweet potato and included disinfection with 0.5, 1, and 2% sodium hypochlorite respectively, while two protocols are proposed in this work and included washing with povidone-iodine, disinfection with sodium hypochlorite 2%; one of these two new protocols also contains acetic acid and quaternary ammonium. For the evaluation of the viability of in vitro plants after disinfection, they were acclimatized in a greenhouse, reintroduced, and a molecular testing by PCR of 16S rRNA gene and ITS was carried out to verify the phytosanitary status of the material. The contaminating microorganisms found were filamentous fungi of the genera Fusarium, Sarocladium, Cladosporium and Aspergillus, yeasts of the genera Pseudozyma and Moesziomyces, and the actinobacterium Curtobacterium sp. The results indicated that washing with povidone-iodine and disinfection with 2% sodium hypochlorite, acetic acid and quaternary ammonium was the most efficient disinfection protocol, reducing the number of contaminated cultures by up to 10% and eradicating 70% of contaminants. The in vitro plants established in the greenhouse remained healthy and, after reintroduction, the molecular test for bacteria and fungi was negative. These results allowed the generation of an optimized protocol that can be incorporated into the in vitro micropropagation process to generate contamination-free sweet potato seeds.

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Telomere length in Agave tequilana Weber plants during the in vitro to ex vitro transition

Cited by 8 publications

References 54 publications

Telomere Length in Norway Spruce during Somatic Embryogenesis and Cryopreservation

Telomere Length in Norway Spruce during Somatic Embryogenesis and Cryopreservation

AteqTERT expression and specific tissue activity in a 2-year-old complete plant in Agave tequilana in field conditions

Development of control strategies for bacteria and fungi associated with a micropropagated new cultivar of orange-fleshed sweet potato (Ipomoea batatas cv. Agrosavia–Aurora)

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