Telomere length in bovine sperm is related to the production of reactive oxygen species, but not to reproductive performance

Ribas-Maynou, Jordi; Llavanera, Marc; Mateo‐Otero, Yentel; Ruiz, Nicolás; Muíño, R.; Bonet, Sergi; Yeste, Marc

doi:10.1016/j.theriogenology.2022.06.025

Cited by 5 publications

(5 citation statements)

References 53 publications

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“…We also confirmed the effects of loci reported by previous studies (Igoshin et al, 2023). Mastitis (Ilska-Warner et al, 2019), bovine leukaemia virus infection (Szczotka et al, 2019), oxidative stress (Ribas-Maynou et al, 2022), parturition and raising the first calf (O'Daniel et al, 2023), lameness (Ilska-Warner et al, 2019), and lactation (Laubenthal et al, 2016) have been found to be associated with cattle telomere length. The management of the farm and genetics are herdrelated factors that can significantly affect telomere length (Brown et al, 2012).…”

Section: Introductionsupporting

confidence: 90%

Influence of breed and environment on leukocyte telomere length in cattle

Yudin,

Igoshin,

Romashov

et al. 2024

Vestn. VOGiS

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High milk yield is associated with reduced longevity in high-producing dairy cattle breeds. Pre-term culling leads to high replacement heifer demand and economic losses for the dairy industry. Selection for this trait is limited because of low heritability and difficulties in phenotype measurement. Telomeres are elements found at the ends of chromosomes, consisting of repetitive DNA sequences, several thousand base pairs in length, coupled with nucleoprotein complexes. Eventually, in humans and most other animals, telomere length reduces with age. When telomeric DNA is truncated to a critical length, cell ageing, cell cycle arrest, and apoptosis are induced. As a result, telomere length can be considered as a predictor of health risks and an individual’s lifespan. The leukocyte telomere length may be used as a proxy phenotype of productive lifespan to improve cattle selection. Our objectives were to assess the effects of breed and breed group (dairy vs. beef) on the leukocyte telomere length and to estimate the effect of cold climate on this trait in Kalmyk cattle populations from the South (Rostov Oblast) and Far North (Republic of Sakha) regions of Russia. The leukocyte telomere lengths were estimated computationally from whole-genome resequencing data. We leveraged data on leukocyte telomere length, sex, and age of 239 animals from 17 cattle breeds. The breed factor had a significant effect on leukocyte telomere length across our sample. There was no difference in leukocyte telomere length between dairy and beef groups. The population factor had a significant effect on leukocyte telomere length in Kalmyk animals. In conclusion, we found that breed, but not breed group (dairy vs. beef), was significantly associated with leukocyte telomere length in cattle. Residence in colder climates was associated with longer leukocyte telomere length in Kalmyk breed cattle.

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Section: Introductionsupporting

confidence: 90%

Influence of breed and environment on leukocyte telomere length in cattle

Yudin,

Igoshin,

Romashov

et al. 2024

Vestn. VOGiS

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“…Although the length of telomeres in male germ cells has been shown to be gradually increasing during spermatogenesis, the level of telomerase activity is decreasing and being restricted [136]. There was no significant difference in sperm quality between Pietrain boars with telomeres of different lengths [106]. There were no observed correlations between sperm TL and quality parameters; however, sperm TL does have a correlation with morulae percentage in the early stage of fertilized oocytes, indicating its potential as a biomarker for embryo development in pigs [137].…”

Section: Pigsmentioning

confidence: 81%

Telomere Dynamics in Livestock

Zhang,

Baker,

Welsh

et al. 2023

Biology

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Telomeres are repeated sequences of nucleotides at the end of chromosomes. They deteriorate across mitotic divisions of a cell. In Homo sapiens this process of lifetime reduction has been shown to correspond with aspects of organismal aging and exposure to stress or other insults. The early impetus to characterize telomere dynamics in livestock related to the concern that aged donor DNA would result in earlier cell senescence and overall aging in cloned animals. Telomere length investigations in dairy cows included breed effects, estimates of additive genetic control (heritability 0.12 to 0.46), and effects of external stressors on telomere degradation across animal life. Evaluation of telomeres with respect to aging has also been conducted in pigs and horses, and there are fewer reports of telomere biology in beef cattle, sheep, and goats. There were minimal associations of telomere length with animal productivity measures. Most, but not all, work in livestock has documented an inverse relationship between peripheral blood cell telomere length and age; that is, a longer telomere length was associated with younger age. Because livestock longevity affects productivity and profitability, the role of tissue-specific telomere attrition in aging may present alternative improvement strategies for genetic improvement while also providing translational biomedical knowledge.

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“…These parameters included viability, acrosome membrane integrity, levels of chromatin (de)condensation, membrane lipid disorder, intracellular calcium levels, total reactive oxygen species (ROS) and intracellular superoxide radicals. The evaluation of all these sperm function parameters has been described in detail elsewhere [ 19 – 23 ]. Briefly, sperm viability was assessed by staining samples with SYBR-14 (32 nmol/L, excited by 488-nm laser and detected through the FITC channel: 525/40 nm) and propidium iodide (PI) (7.5 µmol/L, excited by 488-nm laser and detected through the PC5.5 channel: 690/50 nm) at 38 ºC for 15 min in the dark.…”

Section: Methodsmentioning

confidence: 99%

“…Interestingly, an exclusive paternal inheritance of the aforementioned miRNA is described in mice pointing out the importance of sperm-borne miRNAs for fertility outcomes [ 13 , 18 ]. Although some authors previously suggested that the miR-34 family members may be essential for the development of bovine gametes [ 19 ], however, little is known about the content, expression, and function of other sperm-borne miRNAs in early bovine embryo and its relationship with fertility.…”

Section: Introductionmentioning

confidence: 99%

Expression of miR-138 in cryopreserved bovine sperm is related to their fertility potential

Salas-Huetos,

Ribas-Maynou,

Mateo-Otero

et al. 2023

J Animal Sci Biotechnol

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Background MicroRNAs (miRNAs) are small, single-stranded, non-coding RNA molecules of 22–24 nucleotides that regulate gene expression. In the last decade, miRNAs have been described in sperm of several mammals, including cattle. It is known that miRNAs can act as key gene regulators of early embryogenesis in mice and humans; however, little is known about the content, expression, and function of sperm-borne miRNAs in early bovine embryo. In this study, total sperm RNA was isolated from 29 cryopreserved sperm samples (each coming from a separate bull) using a RNeasy kit and treatment with DNase I. RNA concentration and purity were determined through an Epoch spectrophotometer and an Agilent Bioanalyzer. The expression of 10 candidate miRNAs in bovine sperm (bta-miR-10a, bta-miR-10b, bta-miR-138, bta-miR-146b, bta-miR-19b, bta-miR-26a, bta-miR-34a, bta-miR-449a, bta-miR-495 and bta-miR-7), previously identified in testis and/or epididymis, was evaluated with RT-qPCR. The cel-miR-39-3p was used as a spike-in exogenous control. Nonparametric Mann–Whitney tests were run to evaluate which miRNAs were differentially expressed between bulls with high fertility [HF; non-return rates (NRR) ranging from 39.5 to 43.5] and those with subfertility (SF; NRR ranging from 33.3 to 39.3). Several sperm functionality parameters (e.g., viability, membrane stability or oxygen consumption, among others) were measured by multiplexing flow cytometry and oxygen sensing technologies. Results RNA concentration and purity (260/280 nm ratio) (mean ± SD) from the 29 samples were 99.3 ± 84.6 ng/µL and 1.97 ± 0.72, respectively. Bioanalyzer results confirmed the lack of RNA from somatic cells. In terms of the presence or absence of miRNAs, and after applying the Livak method, 8 out of 10 miRNAs (bta-miR-10b, -138, -146b, -19b, -26a, -449a, -495, -7) were consistently detected in bovine sperm, whereas the other two (bta-miR-10a, and -34a) were absent. Interestingly, the relative expression of one miRNA (bta-miR-138) in sperm was significantly lower in the SF than in the HF group (P = 0.038). In addition to being associated to fertility potential, the presence of this miRNA was found to be negatively correlated with sperm oxygen consumption. The expression of three other miRNAs (bta-miR-19b, bta-miR-26a and bta-miR-7) was also correlated with sperm function variables. Conclusions In conclusion, although functional validation studies are required to confirm these results, this study suggests that sperm bta-miR-138 is involved in fertilization events and beyond, and supports its use as a fertility biomarker in cattle.

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Telomere length in bovine sperm is related to the production of reactive oxygen species, but not to reproductive performance

Cited by 5 publications

References 53 publications

Influence of breed and environment on leukocyte telomere length in cattle

Influence of breed and environment on leukocyte telomere length in cattle

Telomere Dynamics in Livestock

Expression of miR-138 in cryopreserved bovine sperm is related to their fertility potential

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