Telomere length in cervical exfoliated cells, interaction with HPV genotype, and cervical cancer occurrence among high‐risk HPV‐positive women

Chen, Xiaojun; Sun, Wei; Ma, Hongxia; Jin, Guangfu; Hu, Zhibin; Han, Song; Li, Dake; Hang, Dong; Wu, Xiaohua; Li, Ni

doi:10.1002/cam4.2246

Cited by 9 publications

(13 citation statements)

References 39 publications

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“…As a prognostic biomarker, tissue TL outperforms existing clinical risk parameters. Several studies have investigated the relationship between TL and clinical features in squamous cell carcinoma (SCC) [31][32][33]. In cancer tissues, TL is linked to risk factors for oesophageal SCC in consumers of alcohol [31] and to cytologic biomarkers for cervical cancer [32].…”

Section: Discussionmentioning

confidence: 99%

“…Several studies have investigated the relationship between TL and clinical features in squamous cell carcinoma (SCC) [31][32][33]. In cancer tissues, TL is linked to risk factors for oesophageal SCC in consumers of alcohol [31] and to cytologic biomarkers for cervical cancer [32]. Furthermore, in non-melanoma skin cancer, TL was found to be significantly lower in SCC than in basal cell carcinoma, Bowen's disease, or keratosis [33].…”

Section: Discussionmentioning

confidence: 99%

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Telomere shortening in head and neck cancer: association between DNA demethylation and survival

Yamada¹,

Misawa²,

Mima³

et al. 2021

J. Cancer

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A growing body of evidence indicates that telomere dysfunction is a biological marker of progression in several types of cancer. However, the association between head and neck squamous cell carcinoma (HNSCC) and telomere length (TL) remains unknown. We measured the absolute TL levels in a well-characterised dataset of 211 tumoral vs normal tissues obtained from the same patients by quantitative polymerase chain reaction assay. Normalised TL levels were significantly lower in tumour samples than in normal tissue (P < 0.001) and there was a positive correlation between tumour tissue and normal mucosal tissue (R 2 = 0.176, P < 0.001). We were able to distinguish two classes, one with a tumour/normal TL ratio ≤ 0.3 (38.4%), which showed clear telomere erosion, and the other with a tumour/normal TL ratio > 0.3 (61.6%), in which the TL was slightly shorter or longer than that in normal tissue. Notably, the tumour/normal TL ratio was correlated with the likelihood of disease recurrence (P = 0.002), the 5-hydroxymethylcytosine level (P = 0.043), and expression of the ten-eleven translocation (TET) gene (P = 0.043). Our findings show that TL shortening and subsequent low levels of 5-hydroxymethylcytosine and TET expression may contribute to development of HNSCC.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Telomere shortening in head and neck cancer: association between DNA demethylation and survival

Yamada¹,

Misawa²,

Mima³

et al. 2021

J. Cancer

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“…Hence, CC represents one of the most preventable cancers ( Paz Soldan et al, 2008 ). Secondary prevention efforts such as early detection of CC and precursor lesions can reduce the incidence and mortality of it ( Chen et al, 2019b ). CC remains the leading cause of cancer-related death for women worldwide despite advances in prevention, detection, diagnosis, and treatment ( Liu et al, 2019a ), yet the molecular mechanisms underlying CC development and progression are unclear ( Che et al, 2016 ).…”

Section: Introductionmentioning

confidence: 99%

Prognostic value of SOX9 in cervical cancer: Bioinformatics and experimental approaches

Chen

Zeng

et al. 2022

Front. Genet.

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Among gynecological cancers, cervical cancer is a common malignancy and remains the leading cause of cancer-related death for women. However, the exact molecular pathogenesis of cervical cancer is not known. Hence, understanding the molecular mechanisms underlying cervical cancer pathogenesis will aid in the development of effective treatment modalities. In this research, we attempted to discern candidate biomarkers for cervical cancer by using multiple bioinformatics approaches. First, we performed differential expression analysis based on cervical squamous cell carcinoma and endocervical adenocarcinoma data from The Cancer Genome Atlas database, then used differentially expressed genes for weighted gene co-expression network construction to find the most relevant gene module for cervical cancer. Next, the Gene Ontology and Kyoto Encyclopedia of Genes and Genomes enrichment analyses were performed on the module genes, followed by using protein–protein interaction network analysis and Cytoscape to find the key gene. Finally, we validated the key gene by using multiple online sites and experimental methods. Through weighted gene co-expression network analysis, we found the turquoise module was the highest correlated module with cervical cancer diagnosis. The biological process of the module genes focused on cell proliferation, cell adhesion, and protein binding processes, while the Kyoto Encyclopedia of Genes and Genomes pathway of the module significantly enriched pathways related to cancer and cell circle. Among the module genes, SOX9 was identified as the hub gene, and its expression was associated with cervical cancer prognosis. We found the expression of SOX9 correlates with cancer-associated fibroblast immune infiltration in immune cells by Timer2.0. Furthermore, cancer-associated fibroblast infiltration is linked to cervical cancer patients’ prognosis. Compared to those in normal adjacent, immunohistochemical and real-time quantitative polymerase chain reaction (qPCR) showed that the protein and mRNA expression of SOX9 in cervical cancer were higher. Therefore, the SOX9 gene acts as an oncogene in cervical cancer, interactive with immune infiltration of cancer-associated fibroblasts, thereby affecting the prognosis of patients with cervical cancer.

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“…So HPV vaccination can effectively prevent it [9]. As a secondary prevention effort, early screening for CC and precursor lesions can also reduce the incidence and mortality of CC [10].…”

Section: Introductionmentioning

confidence: 99%

Identification of Hub Gene in Cervical Cancer by Weighted Gene Co-Expression Network Analysis

Chen¹,

Huang²,

Zhang³

et al. 2021

Preprint

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Background:Cervical cancer(CC) is one of the most common malignant tumors in gynecology. Both its incidence and mortality are high. Despite advances in screening, diagnosis, prevention, and treatment, CC is still one of the leading causes of cancer-related death in women. However, in the pathogenesis of CC, the exact molecular mechanism is still unclear. It may be a multi-gene, multi-factor, multi-step, and multi-stage complex process. Hence, the pathogenesis and molecular mechanism of CC are the keys to effective treatment of it. In this study, we tried to identify candidate biomarkers for cervical cancer through weighted gene co-expression network analysis (WGCNA). Methods: The gene expression profile of CESC was downloaded from The Cancer Genome Atlas (TCGA) database. Differentially expressed genes (DEGs) were analyzed by the Limma package, and the gene co-expression module was constructed by WGCNA. Use the online website STRING to construct the protein interaction network of genes in significant modules, and then use Cytoscape analysis to find the 10 most important node degree genes. Results: Among these 10 genes, SOX9 expression was associated with the prognosis of cervical cancer patients. Immunohistochemical results from the online website human protein atlas showed that SOX9 protein expression was significantly higher in cervical cancer tissues than in normal cervical tissues. After collecting cancerous and paracancerous tissue specimens from 16 patients with cervical cancer, Q-PCR showed that the mRNA expression levels of SOX9 in cervical cancer tissues were significantly greater than those in normal adjacent tissues. Conclusions: The elevated expression of SOX9 is significantly related to the disease-free survival and overall survival of cervical cancer. Therefore, the SOX9 gene could be used as an indicator of cervical cancer diagnosis and prognosis.

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Telomere length in cervical exfoliated cells, interaction with HPV genotype, and cervical cancer occurrence among high‐risk HPV‐positive women

Cited by 9 publications

References 39 publications

Telomere shortening in head and neck cancer: association between DNA demethylation and survival

Telomere shortening in head and neck cancer: association between DNA demethylation and survival

Prognostic value of SOX9 in cervical cancer: Bioinformatics and experimental approaches

Identification of Hub Gene in Cervical Cancer by Weighted Gene Co-Expression Network Analysis

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