Telomeres shorten while Tert expression increases during ageing of the short-lived fish Nothobranchius furzeri

Hartmann, Nils; Reichwald, Kathrin; Lechel, André; Graf, M.; Kirschner, Jeanette; Dorn, Alexander; Terzibasi, Eva; Wellner, Juliane; Platzer, Matthias; Rudolph, K. Lenhard; Cellerino, Alessandro; Englert, Christoph

doi:10.1016/j.mad.2009.01.003

Cited by 123 publications

(114 citation statements)

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“…All microsatellite markers were homozygous in the GRZ male grandparent, except those linked with sex (see below), confirming that GRZ is an inbred strain ). Forty-one percent of the microsatellite markers (63 of 152) were heterozygous in the MZM-0403 female grandparent, which is consistent with this strain being recently derived from the wild and propagated in captivity for no more than seven generations (Terzibasi et al 2008;Hartmann et al 2009). …”

Section: Resultssupporting

confidence: 76%

“…Nothobranchius furzeri is the shortest-lived species of the Nothobranchius genus, with an intergeneration time of 40 days, a median life span of 9-11 weeks, and a maximum life span of 12-15 weeks for the shortestlived strain GRZ (Valdesalici and Cellerino 2003;Genade et al 2005;Valenzano et al 2006;Terzibasi et al 2008Terzibasi et al , 2009Hartmann et al 2009). Natural populations of N. furzeri can vary substantially in phenotypic traits.…”

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confidence: 99%

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Mapping Loci Associated With Tail Color and Sex Determination in the Short-Lived Fish Nothobranchius furzeri

Valenzano

Kirschner²,

Kamber

et al. 2009

Genetics

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The African fish Nothobranchius furzeri is the shortest-lived vertebrate species that can reproduce in captivity, with a median life span of 9-11 weeks for the shortest-lived strain. Natural populations of N. furzeri display differences in life span, aging biomarkers, behavior, and color, which make N. furzeri a unique vertebrate system for studying the genetic basis of these traits. We mapped regions of the genome involved in sex determination and tail color by genotyping microsatellite markers in the F 2 progeny of a cross between a short-lived, yellow-tailed strain and a long-lived, red-tailed strain of N. furzeri. We identified one region linked with the yellow/red tail color that maps close to melanocortin 1 receptor (mc1r), a gene involved in pigmentation in several vertebrate species. Analysis of the segregation of sex-linked markers revealed that N. furzeri has a genetic sex determination system with males as the heterogametic sex and markedly reduced recombination in the male sex-determining region. Our results demonstrate that both naturally-evolved pigmentation differences and sex determination in N. furzeri are controlled by simple genetic mechanisms and set the stage for the molecular genetic dissection of factors underlying such traits. The microsatellite-based linkage map we developed for N. furzeri will also facilitate analysis of the genetic architecture of traits that characterize this group of vertebrates, including short life span and adaptation to extreme environmental conditions.

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Section: Resultssupporting

confidence: 76%

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confidence: 99%

Mapping Loci Associated With Tail Color and Sex Determination in the Short-Lived Fish Nothobranchius furzeri

Valenzano

Kirschner²,

Kamber

et al. 2009

Genetics

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“…Aging in the turquoise killifish has been extensively characterized at both the phenotypical and molecular level in both the GRZ strain and other strains such as MZM0403 and 0410 (Baumgart et al., 2012, 2014; Di Cicco, Tozzini, Rossi & Cellerino, 2011; Hartmann et al., 2009, 2011; Priami et al., 2015; Terzibasi Tozzini et al., 2014; Terzibasi et al., 2008; Tozzini, Baumgart, Battistoni & Cellerino, 2012). Indeed, despite their short lifespan compared to other vertebrates, various strains of the turquoise killifish recapitulate numerous stereotypical aging traits that have been reported in other vertebrates (Figure 5), including decline in reproduction, fertility, cognition, mobility, regeneration, and tissue homeostasis, along with increased incidence of senescence, neural and muscular degeneration, and cancerous lesions (Di Cicco et al., 2011; Terzibasi, Valenzano & Cellerino, 2007; Terzibasi et al., 2008; Valenzano, Terzibasi, Cattaneo, Domenici & Cellerino, 2006; Wendler, Hartmann, Hoppe & Englert, 2015).…”

Section: The African Turquoise Killifish Lifecycle Is Composed Of Twomentioning

confidence: 99%

“…Importantly, the turquoise killifish does not die of just one disease, but appears to have multiple causes of death in old age, indicating that its lifespan is truly compressed rather than limited by a specific disease. Old turquoise killifish also exhibit molecular markers of aging, such as a decrease in mitochondrial DNA copy number and telomere length with age (Hartmann et al., 2009, 2011). Finally, environmental stimuli known to impact lifespan in other species (e.g., dietary restriction) also extend the lifespan of the turquoise killifish (Terzibasi et al., 2009).…”

Section: The African Turquoise Killifish Lifecycle Is Composed Of Twomentioning

confidence: 99%

“…A good example is the telomerase reverse transcriptase ( TERT ), which when mutated in the turquoise killifish, results in phenotypes that are similar to those seen in humans deficient for telomerase: defects in actively proliferating organs and systems, such as blood, gut, and gonads (Harel et al., 2015; Figure 3). Importantly, the telomeres in the turquoise killifish are more comparable in length to human telomeres (~8 kb) than mouse telomeres (which are extremely long ~50–150 kb; Harel et al., 2015; Hartmann et al., 2009), making this fish a great research system to model and study telomere attrition in the context of aging and telomere‐related pathologies (e.g., age‐dependent stem cell exhaustion) in vivo.…”

Section: Using the African Turquoise Killifish As A Research Organismmentioning

confidence: 99%

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The African turquoise killifish: A research organism to study vertebrate aging and diapause

Brunet²

2018

Aging Cell

135

118

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SummaryThe African turquoise killifish has recently gained significant traction as a new research organism in the aging field. Our understanding of aging has strongly benefited from canonical research organisms—yeast, C. elegans, Drosophila, zebrafish, and mice. Many characteristics that are essential to understand aging—for example, the adaptive immune system or the hypothalamo‐pituitary axis—are only present in vertebrates (zebrafish and mice). However, zebrafish and mice live more than 3 years and their relatively long lifespans are not compatible with high‐throughput studies. Therefore, the turquoise killifish, a vertebrate with a naturally compressed lifespan of only 4–6 months, fills an essential gap to understand aging. With a recently developed genomic and genetic toolkit, the turquoise killifish not only provides practical advantages for lifespan and longitudinal experiments, but also allows more systematic characterizations of the interplay between genetics and environment during vertebrate aging. Interestingly, the turquoise killifish can also enter a long‐term dormant state during development called diapause. Killifish embryos in diapause already have some organs and tissues, and they can last in this state for years, exhibiting exceptional resistance to stress and to damages due to the passage of time. Understanding the diapause state could give new insights into strategies to prevent the damage caused by aging and to better preserve organs, tissues, and cells. Thus, the African turquoise killifish brings two interesting aspects to the aging field—a compressed lifespan and a long‐term resistant diapause state, both of which should spark new discoveries in the field.

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Brain Atlas of an Emerging Teleostean Model: Nothobranchius furzeri

D’Angelo

2013

The Anatomical Record

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Nothobranchius furzeri has emerged as a new fish model for neurobiological and age research over recent years, due to the exceptionally short lifespan, age-dependent cognitive/behavioral decline, expression of agerelated biomarkers. The growing interest in this teleost has raised the need to construct an atlas of the whole brain of N. furzeri. The study has been carried out on adult specimens belonging to the long lived strain, originating from Mozambique and named MZM 04/10. In the atlas, the external features of brain, images of sections stained with luxol fast bleu/violet and schematic drawings of the most representative sections are showed. The identification and description of brain structures has been carried out on methodological and hodological studies. Comparative analyses have

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Telomeres shorten while Tert expression increases during ageing of the short-lived fish Nothobranchius furzeri

Cited by 123 publications

References 49 publications

Mapping Loci Associated With Tail Color and Sex Determination in the Short-Lived Fish Nothobranchius furzeri

Mapping Loci Associated With Tail Color and Sex Determination in the Short-Lived Fish Nothobranchius furzeri

The African turquoise killifish: A research organism to study vertebrate aging and diapause

Brain Atlas of an Emerging Teleostean Model: Nothobranchius furzeri

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