Temperature affects brain and pituitary gene expression related to reproduction and growth in the male blue gouramis, Trichogaster trichopterus

Abstract

This study examined the effect of temperature on reproduction and growth-related factors in blue gourami males under nonreproductive and reproductive conditions. Males that were maintained under nonreproductive conditions did not build nest and the gonado-somatic index (% GSI) was significantly higher in fish maintained at 27°C compared with fish maintained at 23°C. The relative mRNA levels of brain gonadotropin-releasing hormone 3 (GnRH3), pituitary adenylate cyclase-activating polypeptide (PACAP), insulin-like growth factor-1(IGF-1), pituitary β-luteinizing hormone (βLH), and prolactin were significantly higher when the fish were maintained at 27°C than at 23°C or 31°C. β-Follicle-stimulating hormone (βFSH) mRNA levels were significantly lower when maintained at 31°C than at the other temperatures. Nests were observed only in males under reproductive conditions. In these fish, higher mRNA levels of GnRH3, PACAP, βFSH, βLH and prolactin were detected at 27°C, and higher mRNA levels of IGF-1 were detected at 23°C, when compared with other temperature of maintenance or with fish that did not build nest. In conclusion, we propose that temperature has more effect on the transcription of genes, associated with reproduction, than on those pertaining to growth.

Effects of temperature and dietary protein on the growth performance andIGF-ImRNA expression of juvenile mirror carp (Cyprinus carpio)

“…In the present study, the IGF‐I gene expressions of both brain and liver in juveniles mirror carp are correlated to the rearing temperature, as previously reported for other species (David & Degani ,b). The IGF‐I mRNA expressions of both brain and liver were significantly higher at 23 and 28 °C than at 18 °C independently of the protein levels can be explained by higher temperatures increase biochemical activity or production rates that may lead to elevations in circulating IGF‐I.…”

From gametogenesis to spawning: How climate‐driven warming affects teleost reproductive biology

“…Brain expression of g nrh1 was also reduced at higher temperature, whereas g nrh2 was not affected in several species (Elisio et al ., 2012; Shahjahan et al ., 2013, 2017). Responses of brain g nrh3 to high water temperature may also vary, as there was no effect in O. bonariensis and T. niphobles (Elisio et al ., 2012; Shahjahan et al ., 2017) but suppression occurred in the Zebra danio [ Danio rerio (Hamilton 1822)] and T. trichopterus (David & Degani, 2011; Shahjahan et al ., 2013). Downstream, high temperature did not impact fshβ or lhβ subunits in T. trichopterus maintained in reproductive conditions (David & Degani, 2011), nor did it impact expression of these genes in O. bonariensis (though there was a nonsignificant tendency for lower expression) (Soria et al ., 2008).…”

Male Blue Gourami (<i>Trichogaster trichopterus</i>) Nest-Building Behavior Is Affected by Other Males and Females

“…The fish were kept in an aquarium (50 × 50 × 30 cm) at a temperature of 27˚C from November to April under a light regime of 12 h light:12 h darkness [11] until the beginning of the experiments, and were fed an artificial diet (45% protein, 7% fat), supplemented with frozen live food (Artemiasalina). All research involving the fish was approved by the committee in MIGAL dealing with animals and conforms to NIH guidelines.…”

“…The purpose of the present study was to examine the effect of males and females on nest-building blue gourami under experimental conditions [11]. Water of different salinities affected hormones involved in reproduction and male nest-building [12].…”

“…Temperature affects males in building nests under different conditions, and brain and pituitary gene expression related to reproduction and growth is described [11]. Males that are maintained under non-reproductive conditions do not build nests, and the gonado-somatic index (% GSI) is significantly higher in fish maintained at 27˚C compared to fish maintained at 23˚C.…”