Temperature- and hydration-dependence of molecular mobility in seeds

Pissis, P.; Konsta, A.A.; Ratković, S.; Todorović, Saša; Laudat, J.

doi:10.1007/bf01992840

Cited by 12 publications

(15 citation statements)

References 27 publications

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“…Relative mean errors were 75% for the measured TSD current, 72 K for temperature control, and 75% for the heating rate (h). The TSD current method has been described in detail elsewhere [28,29,[36][37][38]. The activation energy of depolarisation (E) on dipole re-orientation was calculated using Eq.…”

Section: Thermally Stimulated Depolarisation Currentmentioning

confidence: 99%

Characterisation of the nanoporous structure of collagen-glycosaminoglycan hydrogels by freezing-out of bulk and bound water

et al. 2006

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Section: Thermally Stimulated Depolarisation Currentmentioning

confidence: 99%

Characterisation of the nanoporous structure of collagen-glycosaminoglycan hydrogels by freezing-out of bulk and bound water

et al. 2006

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“…Properties of water in biological systems were studied extensively using isothermal sorption measurement (Schneider and Schneider, 1972;Clegg, 1978;Lusher-Mattli and Ruegg, 1982;Rupley et al, 1983), calorimetric method (Ruegg et al, 1975;Bakradze and Balla, 1983;Vertucci, 1990), infrared and Raman spectroscopy (Careri et al, 1979;Luck, 1985;Cameron et al, 1988), nuclear magnetic resonance (NMR) spectroscopy (Mathur-de Vre, 1979;Seewaldt et al, 1981;Rorschach and Hazlewood, 1986;Ratkovic, 1987), quasi-elastic neutron-scattering spectroscopy (Lehmann, 1984;Trantham et al, 1984), and dielectric relaxation techniques (Harvey and Hoekstra, 1972;Kamiyoshi and Kudo, 1978;Clegg et al, 1982;Pissis et al, 1987;Bruni and Leopold, 1992;Pissis et al, 1996). These studies revealed the great structural complexity of water in biological systems.…”

mentioning

confidence: 99%

“…Isothermal sorption measurements showed the presence of three hydration regions: a strong water-binding region at low water content (WC), a weak binding region at intermediate WC, and a very loose binding region at high WC (Clegg, 1978;Vertucci and Leopold, 1987). More sensitive differential scanning calorimeter (DSC), NMR, and dielectric techniques identified the existence of at least four or five fractions of water, presumably relating to different interactions between water and cellular constituents (Clegg, 1986;Ratkovic, 1987;Vertucci, 1990;Pissis et al, 1996). These hydration levels are correlated to the onset of various metabolic activities in organisms.…”

mentioning

confidence: 99%

“…This technique is capable of providing information concerning the mobility and rotational freedom of hydration water, hydration sites, and mechanisms (Mascarenhas, 1980;Pissis et al, 1987;Bruni and Leopold, 1992;Pissis et al, 1996). TSC is based upon the dependence of the microdynamics of water dielectric relaxation on their surroundings resulting in different dielectric relaxation times for water in different fractions, and on the influence of water on the dielectric relaxation mechanisms of other biomolecules.…”

mentioning

confidence: 99%

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Dielectric Relaxation of Water and Water-Plasticized Biomolecules in Relation to Cellular Water Organization, Cytoplasmic Viscosity, and Desiccation Tolerance in Recalcitrant Seed Tissues

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2000

Plant Physiology

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To understand the relationship between the organization of cellular water, molecular interactions, and desiccation tolerance, dielectric behaviors of water and water-plasticized biomolecules in red oak (Quercus rubra) seeds were studied during dehydration. The thermally stimulated current study showed three dielectric dispersions: (a) the relaxation of loosely-bound water and small polar groups, (b) the relaxation of tightly-bound water, carbohydrate chains, large polar groups of macromolecules, and (c) the "freezing in" of molecular mobility (glassy state). Seven discrete hydration levels (water contents of 1.40, 0.55, 0.41, 0.31, 0.21, 0.13, and 0.08 g/g dry weight, corresponding to Ϫ1.5, Ϫ8, Ϫ11, Ϫ14, Ϫ24, Ϫ74, and Ϫ195 MPa, respectively) were identified according to the changes in thermodynamic and dielectric properties of water and water-plasticized biomolecules during dehydration. The implications of intracellular water organization for desiccation tolerance were discussed. Cytoplasmic viscosity increased exponentially at water content Ͻ 0.40 g/g dry weight, which was correlated with the great relaxation slowdown of water-plasticized biomolecules, supporting a role for viscosity in metabolic shutdown during dehydration.

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“…Relative mean errors for measured TSD current were ±5%, ±2 K for temperature, and ±5% for the temperature change rate. The TSDC method has been described in detail elsewhere [18][19][20][21][22][23][24]. The distribution function of the activation energy of depolarisation f (E) linked to dipole re-orientations, which cause the TSD current (experimentally measured), was calculated using equation (1) [24]:…”

Section: Tablementioning

confidence: 99%

Interaction of unmodified and partially silylated nanosilica with red blood cells

et al. 2007

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Abstract:Interaction of red blood cells (RBCs) with unmodified and partially (50%) silylated fumed silica A-300 (nanosilica) was studied by microscopic, XRD and thermally stimulated depolarisation current (TSDC) methods. Nanosilica at a low concentration C A−300 < 0.01 wt.% in buffered aqueous suspension is characterised by a weak haemolytic effect on RBCs. However, at C A−300 = 1 wt% all RBCs transform into shadow corpuscles because of 100% haemolysis. Partial (one-half) hydrophobization of nanosilica leads to reduction of the haemolytic effect in comparison with unmodified silica at the same concentrations. A certain portion of the TSDC spectra of the buffered suspensions with RBC/A-300 is independent of the amounts of silica. However, significant portions of the low-and high-temperature TSDC bands have a lower intensity at C A−300 = 1 wt% than that for RBCs alone or RBC/A-300 at C A−300 = 0.01 wt.% because of structural changes in RBCs. Results of microscopic and XRD investigations and calculations using the TSDC-and NMR-cryoporometry suggest that the intracellular structures in RBCs (both organic and aqueous components) depend on nanosilica concentration in the suspension.

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Temperature- and hydration-dependence of molecular mobility in seeds

Cited by 12 publications

References 27 publications

Characterisation of the nanoporous structure of collagen-glycosaminoglycan hydrogels by freezing-out of bulk and bound water

Characterisation of the nanoporous structure of collagen-glycosaminoglycan hydrogels by freezing-out of bulk and bound water

Dielectric Relaxation of Water and Water-Plasticized Biomolecules in Relation to Cellular Water Organization, Cytoplasmic Viscosity, and Desiccation Tolerance in Recalcitrant Seed Tissues

Interaction of unmodified and partially silylated nanosilica with red blood cells

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