Temperature and Milk Composition Responses to Infusion of E. coli LPS and S. aureus Vaccines in the Goat1

Jarman, R. F.; Caruolo, E.V.

doi:10.1111/j.1439-0442.1984.tb01333.x

Cited by 2 publications

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“…In artificially induced experimental mastitis, various doses of LPS for IMM infusion have been used in the literature. Dhondt et al (1977) IMM infused 3 mg of LPS and induced mastitis in dairy cows and goats; Jarman and Caruolo (1984) infused 0.5 mg of LPS into the mammary gland and induced mastitis in dairy goats; Wall et al (2016) infused 0.2 μg of LPS into the mammary gland and induced mastitis in dairy cows; Pu et al (1998), Zhong et al (2008), and Miao et al (2007) used LPS at a dose of 50 μg/kg for IMM infusion and induced mastitis in dairy goats. Because the regions where Pu et al (1998), Zhong et al (2008), and Miao et al (2007) carried out their experiments are closer to our regions, and these regions have similar feeding system and climatic environment, the same dose of LPS as in their experiments (50 μg/kg of BW) was used in the current study.…”

Section: Discussionmentioning

confidence: 99%

Therapeutic effect of ginsenoside Rg1 on mastitis experimentally induced by lipopolysaccharide in lactating goats

Wang

et al. 2019

Journal of Dairy Science

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Escherichia coli is a cause of subclinical and clinical mastitis in dairy cattle and goats, and sometimes causes severe clinical disease that may result in death of the animal. Previous investigation showed that ginsenoside Rg1 extracted from Panax ginseng C.A. Meyer (Araliaceae) has an anti-inflammatory effect on the sepsis induced by E. coli lipopolysaccharide via competitive binding to toll-like receptor 4. We hypothesized that intravenous injection of Rg1 had therapeutic effect on mastitis experimentally induced by intramammary infusion of lipopolysaccharide in lactating goats. In this study, 9 lactating goats were randomly assigned to 1 of the 3 groups: (1) lipopolysaccharide intramammary infusion + saline intravenous injection, (2) lipopolysaccharide intramammary infusion + Rg1 intravenous injection, and (3) saline intramammary administration + saline intravenous injection. Because no adverse clinical signs were observed after intramammary infusion of saline and intravenous injection of Rg1 in a preliminary experiment, and available qualified goats were limited in this study, this treatment was not included in this study. One udder half of each goat received intramammary infusion of lipopolysaccharide (50 μg/kg of body weight; groups 1 and 2) or saline solution (group 3), and the other half was infused with 2 mL of saline solution at h 0. Afterward, intravenous injections of saline solution (groups 1 and 3) or Rg1 (2.5 mg/kg of body weight; group 2) were administered at h 2 and 4 postlipopolysaccharide challenge. Blood and milk samples were collected 3, 6, 9, 12, 15, 18, 21, 24, 48, and 72 h post-lipopolysaccharide challenge, and clinical signs were monitored hourly after lipopolysaccharide challenge within the first 10 h and at the same time points as blood samples. The results showed that Rg1 treatment downregulated rectal temperature, udder skin temperature, udder girth, milk somatic cell count, and N-acetyl-β-d-glucosaminidase and upregulated milk production, lactose, and recovered blood components, such as white blood cells, neutrophils, lymphocytes, total proteins, albumin, and globulin. Considering the positive therapeutic effect on lipopolysaccharideinduced mastitis in goats presented in this study as well as the anti-inflammatory activity found previously, the botanical Rg1 deserves further study as a therapeutic agent in the treatment of E. coli mastitis in dairy animals.

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Section: Discussionmentioning

confidence: 99%

Therapeutic effect of ginsenoside Rg1 on mastitis experimentally induced by lipopolysaccharide in lactating goats

Wang

et al. 2019

Journal of Dairy Science

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Temperature and Milk Composition Responses of the Non‐Infused Mammary Gland to Infusion of E. coli LPS and S. aureus Vaccines and to Intravenous E. coli LPS in the Goat¹

Jarman

Caruolo

1987

Journal of Veterinary Medicine Series A

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Temperature and milk compositional responses were studied in halves of goat udders opposite those infused intramammarily with various agents, as well as following systemic (IV) LPS injection. Infusion of LPS resulted in an average temperature increase for the non-infused glands and colon of around 1.6 "C. Infusion of a lysed S. aureus vaccine resulted in essentially no temperature changes. Infusion of an inactivated S. aweus vaccine resulted in a moderate glandular and colonic temperature increase of 0.5 "C by 5 h. Milk fat percentages and hourly fat production decreased significantly following infusion of each agent. The IV injection of LPS caused glandular and colonic temperature increases of 2.2 "C and 1.8 "C, respectively, by 4 h. In all four trials the milk somatic cell concentration (MSCC) remained unchanged, while protein values showed a non-significant decrease. The intramammary temperature of the non-infused gland paralleled the colonic temperature whether systemic or local administration was used. Comparison between the results obtained for the infused and noninfused glands indicates that temperature increases were similar, as was the depression in milk fat content. Protein and MSCC, however, responded differentially. These results indicate that milk fat percentage may be a more sensitive indicator of a general systemic response than MSCC. 562 JARMAN and CARUOLO increases biphasically (5-7, 9-11, 13), and a decrease occurs in the milk protein concentration (1, 16).The intravenous (IV) administration of LPS causes a systemic febrile response (17,26, 28-31), blood leukopenia followed by a leukocytosis (17, 26, 30), and ruminal hypomotility leading to stasis (26, 27, 29, 30).There is only very limited data published on the response of the non-infused glands to LPS infusion or on the mammary response following IV administration. It has been reported generally that LPS infusion into one gland does not influence the MSCC of the non-infused glands (3, 4, 12, 24), but a recent report (25) indicated that an increase in MSCC from 7.1 mm to 23.3 mm (in mean Wisconsin Mastitis Test score) occurred in the non-infused bovine gland. Also the non-infused gland demonstrated a monophasic increase in MBF following contralateral LPS infusions (7, 9). A similar monophasic Model M, Mini-Mitter Co., Sunriver, Oregon 97702 USA. Models 44TA and 402, Yellow Springs Instrument Co., Inc., Yellow Springs, Ohio 45387 USA.Millex-HA, Millipore Corporation, Bedford, Massachusetts 01 730 USA.' Fossomatic and Milk-0-scan, Foss America, Inc., Fishkill, New York 12524 USA.

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Temperature and Milk Composition Responses to Infusion of E. coli LPS and S. aureus Vaccines in the Goat¹

Cited by 2 publications

References 5 publications

Therapeutic effect of ginsenoside Rg1 on mastitis experimentally induced by lipopolysaccharide in lactating goats

Therapeutic effect of ginsenoside Rg1 on mastitis experimentally induced by lipopolysaccharide in lactating goats

Temperature and Milk Composition Responses of the Non‐Infused Mammary Gland to Infusion of E. coli LPS and S. aureus Vaccines and to Intravenous E. coli LPS in the Goat¹

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Temperature and Milk Composition Responses to Infusion of E. coli LPS and S. aureus Vaccines in the Goat1

Cited by 2 publications

References 5 publications

Therapeutic effect of ginsenoside Rg1 on mastitis experimentally induced by lipopolysaccharide in lactating goats

Therapeutic effect of ginsenoside Rg1 on mastitis experimentally induced by lipopolysaccharide in lactating goats

Temperature and Milk Composition Responses of the Non‐Infused Mammary Gland to Infusion of E. coli LPS and S. aureus Vaccines and to Intravenous E. coli LPS in the Goat1

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Temperature and Milk Composition Responses to Infusion of E. coli LPS and S. aureus Vaccines in the Goat¹

Temperature and Milk Composition Responses of the Non‐Infused Mammary Gland to Infusion of E. coli LPS and S. aureus Vaccines and to Intravenous E. coli LPS in the Goat¹