Temperature and pH characteristics of amylase and proteinase of adult freshwater pearl mussel, Hyriopsis (Hyriopsis) bialatus Simpson 1900

Areekijseree, Mayuva; Engkagul, Arunee; Kovitvadhi, Uthaiwan; Thongpan, Amara; Mingmuang, Mingkwan; Pakkong, Pannee; Rungruangsak‐Torrissen, Krisna

doi:10.1016/j.aquaculture.2003.12.008

Cited by 102 publications

(77 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The detected amounts of products, including L-tyrosine and p-nitroanilide for pepsin and trypsin, were used to calculate relative activities expressed as conversion per min. The determination of α-amylase (EC 3.2.1.1) was based on Areekijseree et al 16 , with slight modifications. The reaction mixture contained 25 µl of 5% soluble starch, 62.5 µl of buffer, 37.5 µl of 20 mmol/l NaCl, and 125 µl of faecal enzyme extract.…”

Section: Characterizations Of Faecal Digestive Enzymesmentioning

confidence: 99%

Faecal characteristics as markers of Chelonia mydas feeding

Thongprajukaew¹,

Kanghae²,

Kittiwattanawong³

2016

ScienceAsia

View full text Add to dashboard Cite

ABSTRACT:Faecal sampling can provide an unobtrusive monitoring of feed utilization. We demonstrate that faecal digestive enzymes and physicochemical characteristics correlate with the feed conversion efficiency in green turtle (Chelonia mydas). The 10-day-old juvenile turtles (25.4 ± 1.3 g body weight) were fed with either fresh feed containing minced fresh fish and vegetable (diet 1); fresh feed containing minced fish fillet, vegetable and fish pellet diet (diet 2); or only fish pellet diet (diet 3) for four months in a completely randomized design (3 treatments × 3 replicates × 10 subjects per replication). Analysis of faecal digestive enzymes (pepsin, trypsin, amylase, and activity ratio of amylase to trypsin) based on colorimetric determinations of specific activity suggested that carbohydrate digestion is a key indicator for feed utilization efficiency in terms of intake and conversion ratio. Physicochemical characteristics to enhance enzymatic hydrolysis, namely, microstructure (scanning electron microscope), thermal transition properties (differential scanning calorimeter), and relative crystallinity (X-ray diffractometer) indicate that the faeces of turtles fed with diet 3 provide a superior feed utilization efficiency. The observed faecal characteristics varied in response to different diets over the duration of the experiments. Thus analysis of faecal digestive enzymes and physicochemical characteristics appear to provide useful unobtrusively sampled indicators for determining feed utilization and can be applied in studies of nutritional status in endangered species without conflicting ethical standards.

show abstract

Section: Characterizations Of Faecal Digestive Enzymesmentioning

confidence: 99%

Faecal characteristics as markers of Chelonia mydas feeding

Thongprajukaew¹,

Kanghae²,

Kittiwattanawong³

2016

ScienceAsia

View full text Add to dashboard Cite

show abstract

“…Nutrient utilization in aquatic animals depends on the activity of their digestive enzymes [1,2]. Digestive processes in fish are less known than in animals, though the data obtained in fish so far show that the digestive enzymes studied are qualitatively similar to those observed in other invertebrates.…”

Section: Introductionmentioning

confidence: 96%

Digestive Enzyme Activities in Different Size Groups and Segments of the Digestive Tract in Labeo Rohita (Day, 1878)

Umalatha¹,

Nimmagadda²,

Jp³

et al. 2016

JAMB

View full text Add to dashboard Cite

“…Specific α-amylase activity was assayed by 3,5-dinitrosalicylic acid (DNS) method according to Areekijseree et al (2004) based on Bernfeld (1951), using 1% soluble starch as substrate. The incubation period lasted 30 min at 37°C.…”

Section: Specific α-Amylase Activitymentioning

confidence: 99%

“…Carbohydrases, such as α-amylase, cellulose, and chitinase, play an important role in the utilization of phytoplankton by bivalves (Gosling, 2003). Among the carbohydrases identified from bivalves, α-amylase (α-1,4-glucan-4-glucanohydrolase; EC 3.2.1.1) catalyzes the endohydrolysis of 1,4-α-Dglucosidic linkages of polysaccharides, and participates in the glycogen utilization of bivalves (Matsui et al, 1996, Areekijseree et al, 2004. In marine bivalves, α-amylase is a limiting enzyme of carbohydrate assimilation (Sellos et al, 2003;Prudence et al, 2006); consequently, the modulation of α-amylase activity affects the assimilation rate and growth of bivalves .…”

Section: Introductionmentioning

confidence: 99%

“…However, knowledge about the molecular characteristics of the α-amylase gene in freshwater mollusks remains limited (Areekijseree et al, 2004). α-Amylase expression is influenced by intrinsic factors, such as hormones and biogenic amines (Giard et al, 1998;Nachman et al, 1999), and extraneous factors, such as food availability (Le Pennec and Le Pennec, 2002;Fernandez-Reiriz et al, 2005;Huvet et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Molecular characterization and expression pattern of an α-amylase gene (HcAmy) from the freshwater pearl mussel, Hyriopsis cumingii

Ren¹,

Tang²,

Wang³

2014

Genet. Mol. Res.

View full text Add to dashboard Cite

ABSTRACT. The freshwater pearl mussel Hyriopsis cumingii is of commercial importance because it produces the freshwater pearl; however, knowledge about the molecular characterization and regulation mechanisms of α-amylase remains unknown for this species. In this study, the full-length cDNA of the α-amylase gene (HcAmy) was isolated from H. cumingii by the rapid amplification of cDNA ends. Tissue-specific expression analysis showed that HcAmy mRNA was mainly expressed in the hepatopancreas; although, the gene was also expressed in the adductor muscle, intestine, gill, and crystalline style. After 2 weeks starvation, the expression of HcAmy mRNA in the hepatopancreas was upregulated at 24 h after re-feeding or when exposed to algal concentration of 32 μg/L chlorophyll-a, indicating that the HcAmy mRNA expression in H. cumingii is regulated by algal availability. The results of this study confirm that the HcAmy gene is an important component of the carbohydrate metabolism of H. cumingii fed phytoplankton. In addition, this study demonstrates that the modulation of this gene is dependent on environmental food availability, including starvation, re-feeding time following a period of starvation, and algal concentrations during re-feeding.

show abstract

Temperature and pH characteristics of amylase and proteinase of adult freshwater pearl mussel, Hyriopsis (Hyriopsis) bialatus Simpson 1900

Cited by 102 publications

References 23 publications

Faecal characteristics as markers of Chelonia mydas feeding

Faecal characteristics as markers of Chelonia mydas feeding

Digestive Enzyme Activities in Different Size Groups and Segments of the Digestive Tract in Labeo Rohita (Day, 1878)

Molecular characterization and expression pattern of an α-amylase gene (HcAmy) from the freshwater pearl mussel, Hyriopsis cumingii

Contact Info

Product

Resources

About