Temperature and storage period over spermatic parameters of jundiá,<i>Rhamdia quelen</i>(Quoy &amp; Gaimard, 1824)

Sanches, Eduardo Antônio; Neumann, Giovano; Toledo, César Pereira Rebechi de; Bombardelli, Robie Allan; Piana, Pitágoras Augusto; Romagosa, Elizabeth

doi:10.1111/j.1365-2109.2011.03056.x

“…) from S. parahybae, obtained in the same season by the same protocol of hormonal manipulation, were similar to these verified in the present experiment (SANCHES et al, 2014). Sanches et al (2013) also determined the characteristics of MOT, VCL, VSL, and VAP in males of S. parahybae using computerized analysis.…”

Section: Discussionsupporting

confidence: 58%

“…Nonetheless, the potential toxicity of such diluents (SANCHES et al, 2011a) and their ability to maintain inactive sperm cells (VIVEIROS et al, 2009) should be taken into account. Semen storage for short periods is successful for preserving the sperm quality in Neotropical fish, as reported in piracanjuba (B. orbignyanus) sperm stored at 4°C and diluted in a proportion of 1:3 (semen:diluent) with BTS (Beltsville Thawing Solution) and KCl (MURGAS et al, 2004).…”

Section: Discussionmentioning

confidence: 99%

“…Besides cryopreservation by freezing, semen storage techniques at low temperatures might favor gamete preservation over short periods (JENKINS;TIERSCH, 1997). These procedures might assure: artificial breeding in cases of asynchronous gamete production (SANCHES et al, 2011a), commercial trade of fresh semen between laboratories and companies, or maintainance of viable gametes from distant collection sites for further laboratory procedures in genetic conservation programs.…”

Section: Introductionmentioning

confidence: 99%

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Seminal and spermatic characteristics of fresh semen and the effects of sperm cooling in Steindachneridion melanodermatum (Garavello, 2005)

Marcos

¹

,

Neumann

²

,

Toledo

³

et al. 2015

SCA

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This study describes the seminal and spermatic characteristics of fresh semen of Steindachneridion melanodermatum and investigates the effects of dilution, temperature, and storage period on its spermatic parameters. Sperm samples were collected from nine hormonally-induced males. The following parameters in fresh sperm were analyzed: seminal plasma osmolality (OSM), seminal pH, sperm motility (MOT), sperm velocity (SV) (including sperm curvilinear velocity (VCL), sperm straight-line velocity (VSL), and sperm average path velocity (VAP)), total time of sperm motility (TEMP), sperm concentration (CONC), and index of sperm normality (NORM). Sperm samples from each male were diluted in a solution containing 5% fructose and 5% powdered milk, and stored at 10°C and 25°C. The same was carried out for sperm samples not subjected to dilution. From these samples, MOT, VCL, VSL, VAP, SV, and TEMP were measured after 0 h, 5 h, 9 h, 18 h, 27 h, 36 h, 45 h, and 54 h. Males released 11.74 ± 5.38 mL of sperm, with an osmolality of 258.78 ± 29.36 mOsm.kg -1 and pH of 7.11 ± 0.31. The sperm presented a MOT of 99.86 ± 0.31% at a concentration of 1.03 × 10 10 ± 3.65 × 10 9 spermatozoa. The results indicate that sperm motility, sperm velocity, and total time of sperm activation were affected by dilution, storage temperature, and storage period (p < 0.05). Procedures for semen storage should be performed with undiluted sperm cooled at 10°C, or kept undiluted at 25°C for up to 27 h. Key words: CASA, endemic, sperm characterization, sperm motility, sperm storage, sperm velocity ResumoO objetivo deste estudo foi descrever os parâmetros seminais e espermáticos no sêmen fresco do Steindachneridion melanodermatum e os efeitos da diluição, da temperatura e do tempo de estocagem do sêmen sobre os parâmetros espermáticos. Foi coletado o sêmen de nove machos, induzidos hormonalmente. No sêmen fresco foram avaliados os parâmetros: osmolaridade do plasma seminal (OSM), pH seminal (pH), motilidade (MOT) e velocidades espermáticas (VCL, VLR, VMD, VE),

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“…The effects of storage temperature on sperm viability in S. melanodermatum are corroborated by the results obtained with sperm stored at 10°C, which extended the cell viability in diluted and non-diluted sperm samples. Sanches et al (2011a) have also suggested that storage of jundiá (R. quelen) sperm under high temperatures is detrimental to sperm motility.…”

Section: Discussionmentioning

confidence: 99%

Seminal and spermatic characteristics of fresh semen and the effects of sperm cooling in Steindachneridion melanodermatum (Garavello, 2005)

Marcos

¹

,

Neumann

²

,

Toledo

³

et al. 2015

Sem. Ci. Agr.

Self Cite

0

View full text Add to dashboard Cite

This study describes the seminal and spermatic characteristics of fresh semen of Steindachneridion melanodermatum and investigates the effects of dilution, temperature, and storage period on its spermatic parameters. Sperm samples were collected from nine hormonally-induced males. The following parameters in fresh sperm were analyzed: seminal plasma osmolality (OSM), seminal pH, sperm motility (MOT), sperm velocity (SV) (including sperm curvilinear velocity (VCL), sperm straight-line velocity (VSL), and sperm average path velocity (VAP)), total time of sperm motility (TEMP), sperm concentration (CONC), and index of sperm normality (NORM). Sperm samples from each male were diluted in a solution containing 5% fructose and 5% powdered milk, and stored at 10°C and 25°C. The same was carried out for sperm samples not subjected to dilution. From these samples, MOT, VCL, VSL, VAP, SV, and TEMP were measured after 0 h, 5 h, 9 h, 18 h, 27 h, 36 h, 45 h, and 54 h. Males released 11.74 ± 5.38 mL of sperm, with an osmolality of 258.78 ± 29.36 mOsm.kg -1 and pH of 7.11 ± 0.31. The sperm presented a MOT of 99.86 ± 0.31% at a concentration of 1.03 × 10 10 ± 3.65 × 10 9 spermatozoa. The results indicate that sperm motility, sperm velocity, and total time of sperm activation were affected by dilution, storage temperature, and storage period (p < 0.05). Procedures for semen storage should be performed with undiluted sperm cooled at 10°C, or kept undiluted at 25°C for up to 27 h. Key words: CASA, endemic, sperm characterization, sperm motility, sperm storage, sperm velocity ResumoO objetivo deste estudo foi descrever os parâmetros seminais e espermáticos no sêmen fresco do Steindachneridion melanodermatum e os efeitos da diluição, da temperatura e do tempo de estocagem do sêmen sobre os parâmetros espermáticos. Foi coletado o sêmen de nove machos, induzidos hormonalmente. No sêmen fresco foram avaliados os parâmetros: osmolaridade do plasma seminal (OSM), pH seminal (pH), motilidade (MOT) e velocidades espermáticas (VCL, VLR, VMD, VE),

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“…The sperm quality is fundamental to the process of artificial reproduction, directly reflecting the success of fertilization and the parameters of sperm motility and morphology are often used as indicators of sperm quality (Figueroa, Valdebenito, & Farias, 2016;Galo et al, 2011;Sanches et al, 2013;Soares, Streit, Ebert, Coldebella, & Oberst, 2010). Motility is essential for fertilization and usually manifests a positive correlation with the fertilization capacity (Figueroa et al, 2016;Sanches et al, 2013). In fish, sperm motility is induced due to contact with water; osmolarity and ionic content of the aquatic environment are central factors in the activation of motility (Figueroa et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

Cryopreservation‐induced morphological changes in the sperm of South American silver catfish ( Rhamdia quelen )

Costa

¹

,

Marques

²

,

Lassen

³

et al. 2019

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The cryopreservation protocols are species‐specific owing to variable sperm sensitivity towards temperature reduction and contact with cryoprotectant solutions. However, little is known about spermatic pathologies, especially after the cryopreservation process. Thus, the objective was to evaluate the effect of cryopreservation on morphological changes in semen of jundiá (Rhamdia quelen). Sperm pool of five males, with >80% motility, as collected, diluted in a cryoprotectant solution and frozen in liquid nitrogen (−196°C). There was a reduction in the percentage of normal cells and sperm motility, accompanied by an increase in the percentage of sperm abnormalities after cryopreservation of R. quelen spermatozoa, indicating a substantial fragility of the spermatozoa towards the cryopreservation process. The most frequent types of morphological changes in the cryopreserved semen were macrocephaly, folded tail, strongly curled tail and distally curled tail. This is the first study to evaluate the spermatic morphology of R. quelen before and after cryopreservation, paving way for further investigations on morphological alterations and for a new classification of these changes in fish semen due to cryopreservation.

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Temperature and storage period over spermatic parameters of jundiá,Rhamdia quelen(Quoy & Gaimard, 1824)

Cited by 16 publications

References 26 publications

Seminal and spermatic characteristics of fresh semen and the effects of sperm cooling in Steindachneridion melanodermatum (Garavello, 2005)

Seminal and spermatic characteristics of fresh semen and the effects of sperm cooling in Steindachneridion melanodermatum (Garavello, 2005)

Seminal and spermatic characteristics of fresh semen and the effects of sperm cooling in Steindachneridion melanodermatum (Garavello, 2005)

Cryopreservation‐induced morphological changes in the sperm of South American silver catfish ( Rhamdia quelen )

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