Temperature Compensated Tests for Raw Milk Supplies: Iii. Further Studies on the Methylene Blue Test

Clegg, L. F. L.; Thomas, S. B.; Griffiths, D. G.; Nokes, E. J.; Tait, Jo; Jones-Evans, Eleanor; Jones, Cathy G.

doi:10.1111/j.1365-2672.1949.tb03882.x

Cited by 4 publications

(4 citation statements)

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“…As suggested by Clegg et al (1949a), and as shown by the present findings, different compensation scales would be desirable for summer and winter. Rowlands & Hosking (1951) stated that a C.O.B.…”

Section: '99supporting

confidence: 62%

“…Campion, Howard & Pischer (1950), reviewing ex-farm milk rejections, maintained that it is possible for any producer to supply milk of a consistently satisfactory quality in any atmospheric conditions likely t.0 be encountered in this country. If it is desired for advisory purposes to indicate unsatisfactory methods of milk production during winter, it may be advisable to use separate temperaturecompensation scales for summer and for winter, as is done a t present in the statutory methylene blue test and as suggested by Clegg et al (1949a) for a temperaturecompensated methylene blue test. The adjustment should not be exaggerated or the results may bear no true relation either to the methods of production or to the actual keeping quality of the milk under commercial conditions.…”

Section: Performance Of Samples In Fhe Temperature-compensated Clot-omentioning

confidence: 99%

“…Although it has been shown that the application of a temperature-compensated test a t 19-20' is practicable under routine conditions of daily sampling and testing there are related points which should be considered. Clegg et al (1949a) found that with a temperature-compensated laethylene blue test the results for mixed milk were inconsistent because of the varying proportions of milk of different meals. But if all milk is required to be of the same keeping quality on collection a t the farm, the sampling of mixed milk should not give rise to difficulties.…”

Section: A Comparison Of Grading By Different Testsmentioning

confidence: 99%

“…IN earlier papers in this series a time-temperature compensation scale for the methylene blue test a t 37" on raw milk supplies was proposed and later applied to 14,000 raw designated milk samples over a 12 months period (Clegg, 1949b;Clegg et al 1949a). The distribution of failures was only slightly more even than that obtained a t the same atmospheric temperature ranges with the 44/54 hr.…”

mentioning

confidence: 99%

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Temperature‐compensated Tests for Raw Milk Supplies. Part Iv. Studies on a Keeping Quality Test at 19–20°

Thomas¹,

Clegg²,

Nokes³

et al. 1951

Proceedings of the Society for Applied Bacteriology

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SUMMARY: A field‐scale experiment carried out in Wales and the West Midlands, in which the temperature‐compensated clot‐on‐boiling (C.O.B.) test of Rowlands & Hosking (1951) was applied to 11,626 samples of designated milk, morning, mixed and evening milk, gave proportions of failures in the ratio 1:2:3 respectively. Despite temperature compensation, failures varied according to atmospheric temperature from c. 50% at 72°F. or more to 10% at 56°F. or less and averaged 20% during the summer and 7% during the six winter months. No marked differences were noted between the keeping qualities of tuberculin tested or accredited milk. During the winter no difference was found between producer‐wholesaler and producer‐retailer supplies, but the latter had a higher proportion of failures of evening and mixed milks during the summer. In a set of 8,297 samples examined in addition by the methylene blue and coli‐aerogenes tests, the C.O.B. test gave a lower incidence of failures than either the methylene blue or combined methylene blue and coli‐aerogenes tests throughout almost the entire year. It is suggested that a temperature‐compensated C.O.B. test is practicable as a routine procedure.

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Section: '99supporting

confidence: 62%

Section: Performance Of Samples In Fhe Temperature-compensated Clot-omentioning

confidence: 99%

Section: A Comparison Of Grading By Different Testsmentioning

confidence: 99%

mentioning

confidence: 99%

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Temperature‐compensated Tests for Raw Milk Supplies. Part Iv. Studies on a Keeping Quality Test at 19–20°

Thomas¹,

Clegg²,

Nokes³

et al. 1951

Proceedings of the Society for Applied Bacteriology

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Dairy Products

DAVIS¹

1968

Quality Control in the Food Industry

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Section B. Bacteriology and mycology applied to dairying

Hiscox¹,

Briggs²

1953

Journal of Dairy Research

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Reviews of the progress of dairy sciencepreformed enzymes, are added to chemically defined substrates, giving clear-cut results. A clockwork automatic bacteriological inoculating device has been devised by Fuson & Marcus (20). An ingenious device(2i), patented in the U.S.A., for counting bacterial colonies, consists of a fountain pen connected to an electro-magnetic counter which registers each time the pen touches the Petri dish or roll-tube. Hannay (22) has described a new type of laterally illuminated colony counting chamber. British Standards for Petri dishes (23) have been issued recently. A microtechnique for the growth of many cultures, particularly for assay work, in one Petri dish has been developed by Turner-Graff(24). Newbould's(25) roll-tube spinner is designed to use cheap, easily obtainable vials or bottles. A suggestion contributing, in the long run, to economy is that of using rigid, seamless tubes of Kraft paper instead of the paper wrappings commonly used for individual sterile pipettes (26); Lack (27) has described a hot box for microcultures, which dispenses with a water jacket since the heating element consists of glass electrothermal heating tape. From America have come details of a simple large-scale anaerobic culture apparatus (28) utilizing a milk can as the main container, and of the uses to which spun glass (29) may be put as an air-filtering medium for bacteriological purposes.The text of the papers read, and the discussion, at a Symposium of the Institute of Biology in London in 1951 have been published under the title Freezing and Drying^); diversity of opinion and lack of collaboration and understanding between bacteriologist and vacuum engineer was apparent concerning even such vital factors as the plugs for drying tubes. Fry & Greaves (31), working mainly with a paracolon bacillus over a 3-year period, have shown that the nature of the suspending medium is one of the most important factors determining the survival rate of bacteria dried in vacuo; the addition of 5-10% of glucose or lactose increased the percentage survival. Young cultures were less resistant to drying than older cultures, but the cell concentration appeared to be of little consequence. ' Mist desiccans' (1 part broth, 3 parts serum with 7-5% added glucose) proved a satisfactory suspending medium for sensitive organisms such as Neisseria gonorrhoea. Briggs' observations (32) on the freeze-drying of lactobacilli are in almost complete agreement with those of Fry & Greaves; using strains of Lactobacillus acidophilus and L. bulgaricus, which were particularly sensitive to freeze-drying, she obtained with her suspending media survival rates of over 10% after 18 months' storage; her work is being continued with other species of lactobacilli subsequently tested to determine whether selection has taken place, although this should not be a danger except where very low survival rates are experienced. Sharpe (33) has tested the precipitin and agglutination reactions of cultures of L. acidophilus and L. bulgaricus which had b...

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Temperature Compensated Tests for Raw Milk Supplies: Iii. Further Studies on the Methylene Blue Test

Cited by 4 publications

References 1 publication

Temperature‐compensated Tests for Raw Milk Supplies. Part Iv. Studies on a Keeping Quality Test at 19–20°

Temperature‐compensated Tests for Raw Milk Supplies. Part Iv. Studies on a Keeping Quality Test at 19–20°

Dairy Products

Section B. Bacteriology and mycology applied to dairying

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