Temperature dependence of carotenoids on C18, C30 and C34 bonded stationary phases

Bell, Christine M.; Sander, Lane C.; Wise, Stephen A.

doi:10.1016/s0021-9673(96)00664-4

Cited by 62 publications

(43 citation statements)

References 54 publications

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“…1b). Non-linear van't Hoff plots may reflect a change in the relative contributions by enthalpy and entropy of transfer of the solute from the mobile phase to the stationary phase [11]. The changes in these 6 relative contributions and possible changes in the retention mechanism are often thought to be related to conformation changes in the stationary phase (e.g.…”

Section: Resultsmentioning

confidence: 99%

Effects of Temperature and Mobile Phase Condition on Chiral Recognition of Poly(l-phenylalanine) Chiral Stationary Phase

et al. 2011

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Characteristic of chiral stationary phase (CSP) with poly(L-phenylalanine) peptide selector, which is in -helical state, was reported. Since environmental factors affect peptide conformation, the changes of enantioselectivity were examined depending on column temperature and mobile phase conditions (ionic strength, pH, mobile phase composition). Column temperature and pH drastically affected the enantioselectivity.Based on these changes, the relation between chiral recognition and secondary structure of the peptide selector was discussed. The column stability during sequential analysis under different separation conditions was also evaluated.

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Section: Resultsmentioning

confidence: 99%

Effects of Temperature and Mobile Phase Condition on Chiral Recognition of Poly(l-phenylalanine) Chiral Stationary Phase

et al. 2011

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“…As a result, non-endcapped polymeric C 30 bonded stationary phases have recently become commercially available, and are especially well suited for separation of isomeric carotenoids, steroids, and retinoids. [31]. This was most probably due to conformal changes of the stationary phase material with temperature.…”

Section: Stationary Phase Considerationsmentioning

confidence: 96%

“…However, temperature programming is not only a compromise with regard to instrumental difficulties with solvent gradients in miniaturized systems, but offers advantages also when working with applications belonging within the middle of the impact area of traditional solvent gradient elution LC. Particularly these are effects of improved efficiency at elevated temperatures [10 -17] and the possibility of advantageously utilizing selectivity effects obtained by varying the temperature [31].…”

Section: Temperature Effectsmentioning

confidence: 99%

Temperature-Programmed Packed Capillary Liquid Chromatography Separation with Large Volume On-Column Focusing of Retinyl Esters

Molander

Thommesen

Bruheim

et al. 1999

J. High Resol. Chromatogr.

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A non‐aqueous isocratic reversed‐phase packed capillary high performance liquid chromatography method for the determination of retinyl esters, utilizing temperature programming and on‐column focusing large volume injection, has been developed. The stationary phase material was C30, and the mobile phase consisted of acetonitrile‐dichloromethane (70 : 30, v/v). A three‐step temperature program, starting at 10°C for 10 min, then 1°/min to 30°C, and finally 2.5°/min to 70°C, was found most appropriate. Compared to an isothermal separation at 25°C, this temperature program provided improved peak resolution, enhanced peak shapes of the last eluting compounds, and a reduction of the overall elution time. A mass limit of detection of 27 pg was found with respect to retinyl palmitate, using UV detection with an “U” shaped flow cell at 327 nm. This corresponds to a concentration limit of detection of 2.7 pg/μL, when utilizing an injection volume of 10 μL. The concentration of retinyl palmitate in arctic seal liver samples was estimated to be 62.6 μg/g liver.

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“…Such effects have previously been an asset in LC separations of complex mixtures of biologically important compounds having varying fatty acids chain lengths and degree of saturation on C 30 stationary phase materials, e. g. carotenoids and retinyl esters [29,30]. The C 30 stationary phase material has also previously demonstrated promising results for disaturated PS species separations as compared to C 18 and polystyrene-divinylbenzene materials using 4.6 mm ID columns [12].…”

Section: Separation Of Ps Speciesmentioning

confidence: 99%

Temperature optimization for improved determination of phosphatidylserine species by micro liquid chromatography with electrospray tandem mass spectrometric detection

Larsen¹,

Molander

2004

J of Separation Science

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A sensitive method for determination of disaturated phosphatidylserine species in the presence of their monounsaturated analogs has been developed, using micro liquid chromatography coupled to electrospray ionization tandem mass spectrometry. The hydrophobic nature of the phosphatidylserine species required a combination of low-eluting sample solvents and sub-ambient temperatures in order to focus large sample volumes up to 20 microL. The samples were dissolved in 2-propanol:hexane:water (20:10:4, v/v/v) prior to 1:9 dilution with ammonium formate buffer:2-propanol:tetrahydrofuran (30:55:15, v/v/v) and final 1:4 dilution with ammonium formate buffer (10 mM):2-propanol: tetrahydrofuran (55:37.5:7.5, v/v/v). The analytical column was a 0.5 x 150 mm stainless steel column packed with 5 microm C30 particles, while the mobile phase contained ammonium formate buffer (10 mM): 2-propanol:tetrahydrofuran (30:55:15, v/v/v). A temperature program from 5 degrees C (hold for 3 minutes) to 75 degrees C at 8 K/min provided separation of the disaturated phosphatidylserine species from their monounsaturated analogs, making available a sensitive determination of the isobaric species. The mass limit of detection for dipalmitoyl phosphatidylserine was 100 pg, corresponding to a concentration limit of detection of 5 pg/microL when using an injection volume of 20 microL. This is an improvement by a factor of 20 as compared to previously reported numbers obtained with conventional LC columns. The within-assay precision of dipalmitoyl phosphatidylserine was 11.9% RSD (n = 3), while the retention time precision was 4.1% RSD (n = 6).

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Temperature dependence of carotenoids on C18, C30 and C34 bonded stationary phases

Cited by 62 publications

References 54 publications

Effects of Temperature and Mobile Phase Condition on Chiral Recognition of Poly(l-phenylalanine) Chiral Stationary Phase

Effects of Temperature and Mobile Phase Condition on Chiral Recognition of Poly(l-phenylalanine) Chiral Stationary Phase

Temperature-Programmed Packed Capillary Liquid Chromatography Separation with Large Volume On-Column Focusing of Retinyl Esters

Temperature optimization for improved determination of phosphatidylserine species by micro liquid chromatography with electrospray tandem mass spectrometric detection

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