Temperature dependence of microhardness of tetragonal hen-egg-white lysozyme single crystals

Koizumi, Hideaki; Tachibana, Masaru; Kawamoto, H.; Kojima, K.

doi:10.1080/14786430410001716791

Cited by 20 publications

(35 citation statements)

References 11 publications

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“…Figure 7 shows indentation marks formed on (110) planes in the three stages. As seen in Figure 7a, the slip traces indicated by arrows around the indentation mark are clearly observed in the incubation stage related to the wet condition, as reported previously [27][28][29]. It is suggested that plastic deformation brought about by indentation mainly results from dislocation multiplication and motion, inducing the slip in the crystal.…”

Section: Evaporation Of Intracrystalline Watersupporting

confidence: 60%

“…The directions of all slip traces indicated by arrows in Figure 7 (a) and (d) are parallel to 001 , as reported previously [27][28][29]. According to the dislocation self-energy in previous papers [28], 110 001 ( = 3.79 nm) and 110 110 ( = 11.1 nm) are suggested as possible slip systems corresponding to 001 slip traces, where is the magnitude of Burgers vector.…”

Section: Dislocations and Peierls Stresssupporting

confidence: 54%

“…The value of the hardness in the incubation stage is found to be 16 MPa, as seen in Figure 1. This value is slightly lower than that reported previously [27][28][29]. The reason can be attributed to the high accuracy for the measurements at controlled humidity.…”

Section: Hardness At Controlled Humiditycontrasting

confidence: 52%

“…This has been also supported by the observation of slip dislocations by X-ray topography [30,31]. The average activation energy of the dislocation motion has been also evaluated to be 0.6 eV from the measurements of the temperature dependence of the indentation hardness [28]. Furthermore, it has been found that the indentation hardness increases with the evaporation of the intracrystalline water in open air where the evaporation time dependence of the hardness has three stages such as incubation, transition, and saturation stages [29].…”

Section: Introductionmentioning

confidence: 57%

“…The studies on plastic properties of protein crystals have been carried out by using Vickers microindentation method, mainly with T-HEWL ones [27][28][29]. In the wet condition, the indentation marks were clearly observed on the (110) crystal plane.…”

Section: Introductionmentioning

confidence: 99%

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Microindentation Hardness of Protein Crystals under Controlled Relative Humidity

et al. 2017

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Vickers microindentation hardness of protein crystals was investigated on the (110) habit plane of tetragonal hen egg-white lysozyme crystals containing intracrystalline water at controlled relative humidity. The time evolution of the hardness of the crystals exposed to air with different humidities exhibits three stages such as the incubation, transition, and saturation stages. The hardness in the incubation stage keeps a constant value of 16 MPa, which is independent of the humidity. The incubation hardness can correspond to the intrinsic one in the wet condition. The increase of the hardness in the transition and saturation stages is well fitted with the single exponential curve, and is correlated with the reduction of water content in the crystal by the evaporation. The saturated maximum hardness also strongly depends on the water content equilibrated with the humidity. The slip traces corresponding to the 110 [110] slip system around the indentation marks are observed in not only incubation but also saturation stages. It is suggested that the plastic deformation in protein crystals by the indentation can be attributed to dislocation multiplication and motion inducing the slip. The indentation hardness in protein crystals is discussed in light of dislocation mechanism with Peierls stress and intracrystalline water.

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Section: Evaporation Of Intracrystalline Watersupporting

confidence: 60%

Section: Dislocations and Peierls Stresssupporting

confidence: 54%

Section: Hardness At Controlled Humiditycontrasting

confidence: 52%

Section: Introductionmentioning

confidence: 57%

Section: Introductionmentioning

confidence: 99%

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Microindentation Hardness of Protein Crystals under Controlled Relative Humidity

et al. 2017

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Molecular Crystals

2009

Chemistry and Physics of Mechanical Hardness

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Brittleness of protein crystals

Nanev

2012

Cryst. Res. Technol.

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A simple technique for studying the brittleness of small crystals is reported. The limits of fracture toughness of tetragonal hen‐egg white lysozyme crystals, oriented with their c‐axis normally to the substrate, were measured. The strong mechanics anisotropy of those crystals was confirmed. The role of the water present in the protein crystal lattice was re‐considered in seek for a more holistic understanding of the process, the idea being that the intra‐crystalline solution sustains the globular protein molecules in their native configuration. Also it is argued that this water may contribute for holding together the huge bio‐molecules in the crystal lattice (that is to act as additional “glue” in the crystal). The hypothesis is that dynamic chains of H‐bonds in the intra‐crystalline water are likely to be prolonged to connect protein‐to‐water‐to‐protein.

show abstract

Temperature dependence of microhardness of tetragonal hen-egg-white lysozyme single crystals

Cited by 20 publications

References 11 publications

Microindentation Hardness of Protein Crystals under Controlled Relative Humidity

Microindentation Hardness of Protein Crystals under Controlled Relative Humidity

Molecular Crystals

Brittleness of protein crystals

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