Temperature-dependent changes in the soil bacterial community in limed and unlimed soil

Abstract

A humus soil with a pH(H(2)O) of 4.9 was limed to a pH of 7.5 and was incubated together with samples from unlimed and field limed (pH 6.1) soils at 5, 20 and 30 degrees C for up to 80 days. The changes in the phospholipid fatty acid (PLFA) pattern were most rapid for the bacterial community of the soil incubated at 30 degrees C, while no changes were found in the soil incubated at 5 degrees C. The response of the community activity to temperature was measured using the thymidine incorporation method on bacteria extracted from the soil. The bacterial community in soil incubated at 30 degrees C became more adapted to high temperature than that in soil samples incubated at 5 degrees C. When soil samples incubated at 30 degrees C and 20 degrees C were returned to 5 degrees C for 35 days, only small changes in the PLFA pattern were found. No significant shift in community temperature adaptation was found. Thus, higher temperatures (with higher turnover) led to higher rates of change in both the PLFA pattern and the activity response to temperature, compared with lower temperatures. No effect of liming as a way of increasing substrate availability and turnover on the rate of change was observed. Changes in the PLFA pattern appeared sooner than changes in the activity response to temperature, indicating that changes in the PLFA pattern were mainly due to phenotypic acclimation and not to species replacement.

Plant-soil interactions and acclimation to temperature of microbial-mediated soil respiration may affect predictions of soil CO2 efflux

“…maintenance of membrane fluidity or synthesis of cold-tolerant enzymes (Margesin et al 2007). Moreover, microbial community structure changes under contrasting temperatures regimes, favoring microbes better adapted to the new temperatures (Zogg et al 1997;Pettersson and Bååth 2003;Firestone 2004, 2006). Therefore it is possible that soil microbial community under cold conditions may have developed mechanisms of acclimation to these sub-optimal temperatures (cold-acclimation).…”

Temperature responses of carbon monoxide and hydrogen uptake by vegetated and unvegetated volcanic cinders

“…Several previous studies (Ranneklev and Bååth, 2001;Pettersson and Bååth, 2003;Hartley and Hopkins, 2008;Bárcenas-Moreno et al, 2009) have demonstrated that soil microbial communities can adapt to elevated temperature regimes. For example, using growth rate as a variable, Ranneklev and Bååth (2001) have shown that heating peat soil from 25-55 1C resulted in community adaptation with optimal growth at 55 1C in only 3 days.…”

Detection of Effects of a High Trophic Level Predator, Sorex unguiculatus (Soricidae, Mammalia), on a Soil Microbial Community in a Cool Temperate Forest in Hokkaido, Using the ARISA Method

“…About 30 , however, sampling was not conducted, since bacterial activity is so low as not to show any substantial changes in community structure during winter in cool-temperate regions (41,42) and soil is frozen during winter in TOEF. Soil samples were packed in plastic bags and kept in a cool icebox.…”