Temperature-dependent osmotic behavior of germinal vesicle and metaphase II stage bovine oocytes in the presence of Me2SO in relationship to cryobiology

Ağca, Yüksel; Liu, J.; Critser, E.S.; McGrath, John; Critser, John K.

doi:10.1002/(sici)1098-2795(199905)53:1<59::aid-mrd7>3.0.co;2-e

Cited by 12 publications

(5 citation statements)

References 42 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Activation energy for DMSO and EG was calculated to be 20.80 kcal/mol and 21.78 kcal/ mol for MII human oocytes (28)(29)(30). Interestingly, the corresponding estimated value for MII bovine oocytes is 21.0 kcal/ mol (31). Thus, we selected MII bovine oocytes to establish a base for MII human oocytes that have undergone laser-assisted slitting and polar body biopsy before their cryopreservation via vitrification technique.…”

Section: Discussionmentioning

confidence: 99%

High survival rate of metaphase II human oocytes after first polar body biopsy and vitrification: determining the effect of previtrification conditions

Keskintepe

Ağca

Sher

et al. 2009

Fertility and Sterility

View full text Add to dashboard Cite

Section: Discussionmentioning

confidence: 99%

High survival rate of metaphase II human oocytes after first polar body biopsy and vitrification: determining the effect of previtrification conditions

Keskintepe

Ağca

Sher

et al. 2009

Fertility and Sterility

View full text Add to dashboard Cite

“…We adopted the 2-parameter model (vs. the 3-parameter model) as it has been argued that this model is more parsimonious, and many previous investigations into membrane permeability of cells including oocytes have shown that the interaction factor (σ) is insignificant (46,56). This model uses a pair of coupled, linear ordinary differential equations to describe the change in cell water volume and moles of intracellular permeating solute (e.g.…”

Section: Experiments 1: Oocyte Permeability To Ethylene Glycolmentioning

confidence: 99%

Human oocyte vitrification: the permeability of metaphase II oocytes to water and ethylene glycol and the appliance toward vitrification

Mullen

Mei

et al. 2008

Fertility and Sterility

View full text Add to dashboard Cite

Objectives-To determine the permeability of human metaphase II oocytes to ethylene glycol and water in the presence of ethylene glycol, and to use this information to develop a method to vitrify human oocytes.Design-An incomplete randomized block design was used for this study.Setting-A University-affiliated assisted reproductive center.Patients-Women undergoing assisted reproduction in the Center for Reproductive Medicine at Shandong University.Interventions-Oocytes were exposed to 1.0 molar ethylene glycol in a single step, and photographed during subsequent volume excursions.Main outcome measures-A 2-parameter model was employed to estimate the permeability to water and EG.Results-Water permeability ranged from 0.15 to 1.17 µm/(min·atm), and ethylene glycol permeability ranged from 1.5 to 30 µm/min between 7 °C at 36 °C. The activation energies for water and ethylene glycol permeability were 14.42 Kcal/mol and 21.20 Kcal/mol, respectively. Publisher's Disclaimer: This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final citable form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.Some of the results from this study were presented at the 62 nd annual meeting of the American Society for Reproductive Medicine, 21-25 October 2006, in New Orleans, LA USA. Conflict of Interest: None. CapsuleHuman metaphase II oocyte permeability to ethylene glycol was determined. A method for vitrification, based upon fundamental principles, was developed from these data. Conclusions-Despite the lower permeability of human MII oocytes to ethylene glycol compared to previously published values for propylene glycol and dimethylsulfoxide, methods to add and remove human oocytes with a vitrifiable concentration of ethylene glycol can be designed which prevent excessive osmotic stress and minimize exposure to high concentrations of this compound. NIH Public Access

show abstract

“…The appropriate cryobiological and biophysical parameters for mature bovine oocytes at 22°C have been previously reported. 23,40,41 Finally, the osmolality was related to the probability of spindle disruption using equation [1]. Since all of the oocytes at the treatment levels of 2700 and 3000 mOsm showed a disrupted spindle, the analysis for the hypertonic data were restricted to the treatments between 280 and 2700 mOsm.…”

Section: Statistical Analysis and Model Developmentmentioning

confidence: 99%

Modeling the Probability of MII Spindle Disruption in Bovine Oocytes as a Function of Total Osmolality Using Logistic Regression and Its Application toward Improved CPA Addition and Removal Procedures

Mullen¹,

Ağca²,

Critser³

2004

Cell Preservation Technology

View full text Add to dashboard Cite

During cryopreservation, cells undergo volume excursions as a result of exposure to anisosmotic solutions containing cryoprotective agents. Cryopreservation methods can be engineered to maintain cell volumes within their osmotic tolerance limits. In this study we investigated the osmotic tolerance limits of the bovine oocyte oolemma and metaphase II spindle. Oocytes were exposed to solutions (75-3000 milliosmolal) for 10 min, then cultured for 1 h. Oolemma integrity was assessed visually prior to fixation. The structure of the metaphase II spindle was assessed using immunocytochemistry and laser scanning confocal microscopy. Logistic regression was used to model the probability of spindle disruption as a function of solution osmolality. Few cells experienced oolemma rupture or spindle disorganization between 200 and 1800 milliosmolal. Higher levels of osmotic stress caused rupture of the oolemma and disruption of the spindle structure. These results can be applied to improve methods for the addition and removal of cryoprotective agents that will protect the spindle from osmotic damage.

show abstract

Temperature-dependent osmotic behavior of germinal vesicle and metaphase II stage bovine oocytes in the presence of Me2SO in relationship to cryobiology

Cited by 12 publications

References 42 publications

High survival rate of metaphase II human oocytes after first polar body biopsy and vitrification: determining the effect of previtrification conditions

High survival rate of metaphase II human oocytes after first polar body biopsy and vitrification: determining the effect of previtrification conditions

Human oocyte vitrification: the permeability of metaphase II oocytes to water and ethylene glycol and the appliance toward vitrification

Modeling the Probability of MII Spindle Disruption in Bovine Oocytes as a Function of Total Osmolality Using Logistic Regression and Its Application toward Improved CPA Addition and Removal Procedures

Contact Info

Product

Resources

About