Temperature-dependent transformation of biogas-producing microbial communities points to the increased importance of hydrogenotrophic methanogenesis under thermophilic operation

Temperature affects microbial abundance, activity and interactions in anaerobic digestion

“…Temperature affects AD performance through modulating microbial community composition and diversity, activities and their interactions, altering the biochemical conversion pathways and thermodynamic equilibrium of the biochemical reactions (Wilson et al, 2008). Due to different adaption potential of different microorganisms to temperature, microbial community composition and abundance will shift with the changes in temperature, which can result in a shift of both predominated methanogenic pathway and process stability (Pap et al, 2015). For example, in AD systems, importance of hydrogenotrophic methanogenesis increases as temperature shifts from 37°C to 55°C, which is based on the observed decrease of acetoclastic Methanosaeta and the increase of the hydrogenotrophic Methanothermobacter and Methanoculleus at elevated temperature (Pap et al, 2015).…”

“…Due to different adaption potential of different microorganisms to temperature, microbial community composition and abundance will shift with the changes in temperature, which can result in a shift of both predominated methanogenic pathway and process stability (Pap et al, 2015). For example, in AD systems, importance of hydrogenotrophic methanogenesis increases as temperature shifts from 37°C to 55°C, which is based on the observed decrease of acetoclastic Methanosaeta and the increase of the hydrogenotrophic Methanothermobacter and Methanoculleus at elevated temperature (Pap et al, 2015). In addition, the phylum Firmicutes increase instead of the Bacteroidetes (Pap et al, 2015), which most likely induces changes in the functional roles of microorganisms .…”

“…For example, in AD systems, importance of hydrogenotrophic methanogenesis increases as temperature shifts from 37°C to 55°C, which is based on the observed decrease of acetoclastic Methanosaeta and the increase of the hydrogenotrophic Methanothermobacter and Methanoculleus at elevated temperature (Pap et al, 2015). In addition, the phylum Firmicutes increase instead of the Bacteroidetes (Pap et al, 2015), which most likely induces changes in the functional roles of microorganisms . Temperature shifts the abundance and activity of specific populations, which determines the roles of specific taxa in the AD food chain including four steps: substrate hydrolysis, acidogenesis, acetogenesis, and methanogenesis.…”

Temperature regulates methane production through the function centralization of microbial community in anaerobic digestion

“…Temperature is one of most important factors that affect stability and performance of anaerobic digestion (AD) process (De Vrieze et al, 2015;Pap et al, 2015). In AD systems, thermodynamic equilibrium of the biochemical reactions (Wilson et al, 2008) and microbial community structure, activities and diversity (Gannoun et al, 2016;Gunnigle et al, 2015;Sun et al, 2015) can be influenced by temperature.…”

“…For example, methanogens show higher growth rates at thermophilic condition, which may induce efficient methane production (Sun et al, 2015;Weiland, 2010). An increase of hydrogenotrophic methanogens at 55°C compared to 35°C (Tian et al, 2015), may result in alternative methanogenic pathways in AD Pap et al, 2015). However, most of these studies focus on microbial community composition, but rarely on metabolic activities inferred from microbial community gene expression.…”