Temperature effects on osmotic fragility, and the erythrocyte membrane

Richieri, Gary V.; Mel, Howard C.

doi:10.1016/0005-2736(85)90343-8

Cited by 39 publications

(13 citation statements)

References 24 publications

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“…The combination of these observations is indicative of seasonal haematopoetic changes in the fish. Cold temperatures are known to increase osmotic fragility by inducing less flexibility in the cytoskeleton of erythrocytes (Jacobs & Parpart, 1931;Richieri & Mel, 1985;Bucx et al, 1988). The relationship to lowered haematocrit could result from mechanical damage incurred due to difficulty in passage through capillary beds.…”

Section: Discussionmentioning

confidence: 99%

Seasonal changes in erythrocyte osmotic fragility and haematocrit in American plaice infected with Haemohormidium terranovae

Siddall

Measures

Desser

1995

Journal of Fish Biology

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American plaice held in captivity for a period of 8 months over winter exhibited increasing erythrocyte osmotic fragility and decreasing haematocrit values until December. In December, osmotic fragility parameters and haematocrit were strongly correlated, suggesting anaemia due to disruption of circulating erythrocytes. Intensity of infection with Haemohormidium terranovae increased through the winter months until March but was not correlated either with osmotic fragility or haematocrit. These results are explained in terms of compensatory haemopoetic changes occurring in plaice. Mortality was apparently unrelated to any of the parameters investigated. 1995 The Fisheries Society of the British Isles

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Section: Discussionmentioning

confidence: 99%

Seasonal changes in erythrocyte osmotic fragility and haematocrit in American plaice infected with Haemohormidium terranovae

Siddall

Measures

Desser

1995

Journal of Fish Biology

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“…Therefore, under such conditions, the interpretation they give of the corresponding osmotic response is by no means unique, and the results might equally well be explained on the basis of the physical-chemical effects induced by those treatments. Furthermore, we should emphasize that the present shape-factor approach has thus far been demonstrated only for normal, native red cells at temperatures no higher than 40°C (Richieri and Mel, 1985b) and cannot be expected to apply, as such, to cells treated in this manner.…”

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confidence: 88%

Alternative interpretation for the osmotic response of human erythrocytes

Massaldi

Richieri

Mel

1986

Journal Cellular Physiology

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In a recent publication, Heubusch et al. (J Cell. Physiol, 122:266-272, 1985) reported changes of erythrocyte volume measured by the Coulter counter technique over a wide range of osmolalities (160 to 3000 m0sm). Their results showed a partially hindered, nonlinear response, in contrast to classical observations made over more restricted osmolality ranges, using other methods. The authors suggested the underlying cause of this behavior to be a mechanical resistance of the membrane cytoskeleton. In this paper, we wish to offer a different interpretation of their results on erythrocyte osmotic behavior, based on similar experiments carried out in our laboratory, and supported by previous analyses from the literature. In particular, it is shown that the shape-factor correction to the electronic sizing measurement can correctly account for the observed deviations from linearity in the hypotonic range. In contrast, increased chemical nonideality and eventual hemolysis are the likely factors responsible for the behavior in the hypertonic range.

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“…Another source of MEF variation related to methodology for EOF could be the environmental temperature during incubation. Incubation temperatures of 29 and 45 0 C produced comparable EOF, but colder temperature of 10 0 C increased EOF (Oyewale, 1991) as a result of swelling of erythrocytes in cold temperature (Richieri & Mel, 1985). EOF increased with incubation temperature (Aloni et al, 1977), but remained stable without any variation at incubation temperatures of 37-48 0 C ( Van der Walt & Russell, 1978).…”

Section: Resultsmentioning

confidence: 99%

Phenotypic drift in osmotic fragility of Sahel goat erythrocytes associated with variability of median fragility

Igbokwe¹,

Ojo²,

Igbokwe³

2015

Sokoto J. Vet. Sc.

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A typical mammalian erythrocyte fragility phenotype (EFP) exhibits a sigmoidal curve of the dependence of fragilities (% haemolysis) on hypotonic saline concentrations, but the goat EFP tends to be hyperbolic. Physiological variation in median erythrocyte fragility (MEF) and the associated EFP of Sahel goats was investigated. Erythrocyte osmotic fragility (EOF) was determined in hypotonic saline using heparinized venous blood from 47 goats (23 males and 24 non-pregnant dry females) aged 1-4 years and weighing 18.87 ± 6.32 (9-30) kg. Packed cell volume (PCV), erythrocyte count and mean corpuscular volume (MCV) were also estimated. Low, medium and high EFP were based on MEF of < 7, 7-8 and > 8 g/L, respectively. MEF of all the goats was 7.5 ± 0.6 (5.9-8.4) g/L. Phenotypic drift from sigmoidal to hyperbolic EOF curve was observed at the lower and upper limits of the phenotypic variation. Frequencies of occurrence of low, medium and high EFP were not different (p > 0.05) between males and females. Fragiligrams of low, medium and high EFP separated at erythrocyte fragilities of 10-70% and saline tonicity of 7-8 g/L. The saline concentrations causing fragilities of 10-70% differed (p < 0.05) among the phenotypes. There was no correlation (r = -0.28, -0.30; p > 0.05) between MEF and MCV or PCV, and between PCV and MCV. In conclusion, phenotypic drift in EOF occurred in Sahel goats without influence by erythrocyte parameters and represented the physiological variability of EOF endpoint estimates that would serve as reference limits in evaluation of erythrocyte membrane defects.

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Temperature effects on osmotic fragility, and the erythrocyte membrane

Cited by 39 publications

References 24 publications

Seasonal changes in erythrocyte osmotic fragility and haematocrit in American plaice infected with Haemohormidium terranovae

Seasonal changes in erythrocyte osmotic fragility and haematocrit in American plaice infected with Haemohormidium terranovae

Alternative interpretation for the osmotic response of human erythrocytes

Phenotypic drift in osmotic fragility of Sahel goat erythrocytes associated with variability of median fragility

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