Temperature Effects on Retention in Reversed-phase Liquid Chromatography of Nucleosides and Their Bases Using Water as the Mobile Phase

Hu, Wenzhi; Hasebe, Kyoshi; Haddad, Paul R.

doi:10.1039/a705426b

Cited by 9 publications

(3 citation statements)

References 35 publications

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“…The nucleosides and their bases were chosen as model analytes because they have the characteristic of being typical hydrogenbonding compounds and can also be separated using a conventional ODS stationary phase with water as the mobile phase. 10 Typical chromatograms obtained using the unmodified ODS (bottom), ODS modified with 1-propanol (ODS-PrOH) (middle) and ODS modified with 1-butanol (ODS-BuOH) (top) as the stationary phase are given in Fig. 1.…”

Section: Separation Of Hydrogen-bonding Analytesmentioning

confidence: 99%

Retention and selectivity effects of coating octadecylsilica stationary phases with low molecular weight alcohols in reversed-phase liquid chromatography using water as mobile phase

Hu¹,

Haddad²

1998

Anal. Commun.

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A simple but useful experimental method for observation of the effects of organic modifiers in the stationary phase in reversed-phase liquid chromatography (RPLC) is reported. An octadecylsilica (ODS)-packed column, used before and after treatment with low molecular weight alcohols (methanol, ethanol, 1-propanol, and 1-butanol), was employed for analysis of two groups of water-soluble model analytes by RPLC with water as the mobile phase. The first group was a mixture of 14 species of nucleosides and their bases and was used to represent hydrogen-bonding analytes, whilst the second group was a mixture of seven alkylsulfonates used to represent hydrophobic analytes. For both groups of analytes, alcohol-modified ODS stationary phases gave higher resolution and a shorter retention time than untreated ODS. These differences in chromatographic performance showed that the alcohol was adsorbed onto the stationary phase and influenced the elution of the target analytes. The adsorbed alcohol is postulated to decrease the overall hydrophobicity of the stationary phase and also to introduce a hydrogen-bonding interaction with suitable analytes. The resultant changes in retention and separation selectivity exhibited by the alcohol-modified stationary phases are dependent on the relative magnitudes of these effects. This behaviour is illustrated using the two groups of target analytes.

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Section: Separation Of Hydrogen-bonding Analytesmentioning

confidence: 99%

Retention and selectivity effects of coating octadecylsilica stationary phases with low molecular weight alcohols in reversed-phase liquid chromatography using water as mobile phase

Hu¹,

Haddad²

1998

Anal. Commun.

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“…Unfortunately, such greener solvent is usually less effective (8). The most interesting idea is to use pure water as a mobile phase (9,10). This idea may be realized at a normal, elevated and subcritical temperature (11)(12)(13)(14)(15)(16)(17)(18)(19)(20).…”

Section: Introductionmentioning

confidence: 99%

“…The separation may be done on commercial C18 or C8 stationary phases, however, a phase collapse effect and retention loss may be observed. Other assumption is that separation may be done with the addition of surfactant to pure water or by performing the procedure in elevated or subcritical temperature (9,10,28).…”

Section: Introductionmentioning

confidence: 99%

The separations using pure water as a mobile phase in liquid chromatography using polar-embedded stationary phases

Bocian

Krzemińska

2019

Green Chemistry Letters and Reviews

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Following the idea of green chemistry, especially green analytical chemistry, a series of stationary phases was synthesized. The obtained materials connect polar and hydrophobic groups in the structure of bonded ligands. These specific surface properties provide the stability of the stationary phase in pure water as a mobile phase. To confirm the solvation ability in purely aqueous mobile phases, excess isotherms of water and acetonitrile were determined. Further, the mixtures of nucleosides, nucleic bases and purine alkaloids were applied to test the separation selectivity of stationary phases in purely aqueous conditions at ambient temperature without any additives to the mobile phase. Among the four tested stationary phases, it is possible to find one for separation of each group of analytes that offers selective separation in reasonable time. The presented data confirms that it is possible to synthesize stationary phases for the separation of target mixtures in pure water conditions.

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Copolymer-grafted silica phase from a cation–anion monomer pair for enhanced separation in reversed-phase liquid chromatography

et al. 2014

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This work reports a new imidazolium and L-alanine derived copolymer-grafted silica stationary phase for ready separation of complex isomers using high-performance liquid chromatography (HPLC). For this purpose, 1-allyl-3-octadecylimidazolium bromide ([AyImC18]Br) and N-acryloyl-L-alanine sodium salt ([AAL]Na) ionic liquids (IL) monomers were synthesized. Subsequently, the bromide counteranion was exchanged with the 2-(acrylamido)propanoate organic counteranion by reacting the [AyImC18]Br with excess [AAL]Na in water. The obtained IL cation-anion monomer pair was then copolymerized on mercaptopropyl-modified silica (Sil-MPS) via a surface-initiated radical chain-transfer reaction. The selective retention behaviors of polycyclic aromatic hydrocarbons (PAHs), including some positional isomers, steroids, and nucleobases were investigated using the newly obtained Sil-poly(ImC18-AAL), and octadecyl silylated silica (ODS) was used as the reference column. Interesting results were obtained for the separation of PAHs, steroids, and nucleobases with the new organic phase. The results showed that the Sil-poly(ImC18-AAL) presented multiple noncovalent interactions, including hydrophobic, π-π, carbonyl-π, and ion-dipole interactions for the separation of PAHs and dipolar compounds. Only pure water was sufficient as the mobile phase for the separation of the nucleobases. Ten nucleosides and bases were separated, using only water as the mobile phase, within a very short time using the Sil-poly(ImC18-AAL), which is otherwise difficult to achieve using conventional hydrophobic columns such as ODS. The combination of electrostatic and hydrophobic interactions are important for the effective separation of such basic compounds without the use of any organic additive as the eluent on the Sil-poly(ImC18-AAL) column.

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Temperature Effects on Retention in Reversed-phase Liquid Chromatography of Nucleosides and Their Bases Using Water as the Mobile Phase

Cited by 9 publications

References 35 publications

Retention and selectivity effects of coating octadecylsilica stationary phases with low molecular weight alcohols in reversed-phase liquid chromatography using water as mobile phase

Retention and selectivity effects of coating octadecylsilica stationary phases with low molecular weight alcohols in reversed-phase liquid chromatography using water as mobile phase

The separations using pure water as a mobile phase in liquid chromatography using polar-embedded stationary phases

Copolymer-grafted silica phase from a cation–anion monomer pair for enhanced separation in reversed-phase liquid chromatography

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