Temperature effects on solute accumulation by Candida utilis

Carbon‐13 nuclear magnetic resonance spectroscopy of living cells and their metabolism of a specifically labeled 13C substrate

“…In particular saturated fatty acids, and oleic and palmitoleic acids the resonance at -6.8 ppm (no. 9) is in the location ex-are the predominant unsaturated fatty acids [ 16,171. pected for the (Y carbon of polypeptides, resonances in The peak at 33 ppm (no.…”

Lipids of yeasts.

“…9). Growth of C. utilis in either batch culture (80) or in the chemostat at fixed growth rate (40,193) results in higher proportion of 16:1 and 18:3 acids at 10 C compared to 30 C. The specific alterations in 16:0, 18:1 and 18:2 acids are subject to limitation of glucose or nitrogen in the medium (193). A pronounced increase of 18:2 acid has been determined (102) in C. tropicalis grown at 28 C compared to 38 C. Cyclic variation of 18:1 and 18:3 acids has been observed (193) in a psychrophilic Candida sp., but is Changes in fatty acid composition of (A) total lipids, (B) phosphatidyl choline, and (C) phosphatidylethanolamine at25 C and 10 C. (Reproduced from reference 148 by kind permission of M. Kates and the Canadian Journal of Biochemistry.)…”

Carbon-13 Fourier transform nuclear magnetic resonance studies of fractionated Candida utilis membranes

“…Characterization of the Samples under Study. The lipid composition of a number of different strains of Candida utilis has been investigated (Dawson and Craig, 1966;Babij et al, 1969;Matile et al, 1969;Hunter and Rose, 1969;Farrell and Rose, 1971;Johnson et al, 1972). In general, it has been found that the bulk of the lipids present are localized in the cellular membranes, specifically the plasmalemma, internal endoplasmic reticulum, and the vacuolar, nuclear, and mitochondrial membranes.…”