Temperature Effects on the Depuration of Vibrio parahaemolyticus and Vibrio vulnificus from the American Oyster (Crassostrea virginica)

Chae, Minjung; Cheney, Daniel P.; Su, Yi‐Cheng

doi:10.1111/j.1750-3841.2008.01031.x

Cited by 62 publications

(49 citation statements)

References 24 publications

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“…We speculate that the answer may lie in results obtained by our laboratory and many others over the last 30 years. Studies examining the uptake and depuration of V. vulnificus in seeded oysters have all reported that V. vulnificus cells are rapidly taken up but are not retained and are quickly depurated (3,5,6,12,14,31,35). If the oyster microflora is firmly established during the early stages of oyster development, transient bacterial cells acquired through gill filtration could be unable to establish residency in the oyster gut.…”

Section: Discussionmentioning

confidence: 99%

Apparent Loss of Vibrio vulnificus from North Carolina Oysters Coincides with a Drought-Induced Increase in Salinity

Froelich

Williams

Noble

et al. 2012

Appl Environ Microbiol

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ABSTRACTDespite years of successful isolation ofVibrio vulnificusfrom estuarine waters, beginning in 2007, it was extremely difficult to cultureV. vulnificusfrom either North Carolina estuarine water or oyster samples. After employing culture-based methods as well as PCR and quantitative PCR for the detection ofV. vulnificus, always with negative results, we concluded that this pathogen had become nearly undetectable in the North Carolina estuarine ecosystem. We ensured that the techniques were sound by seeding North Carolina oysters withV. vulnificusand performing the same tests as those previously conducted on unadulterated oysters.V. vulnificuswas readily detected in the seeded oysters using both classes of methods. Furthermore, oysters were obtained from the Gulf of Mexico, andV. vulnificuswas easily isolated, confirming that the methodology was sound but that the oysters and waters of North Carolina were lacking theV. vulnificuspopulation studied for decades. Strikingly, the apparent loss of detectableV. vulnificuscoincided with the most severe drought in the history of North Carolina. The drought continued until the end of 2009, with an elevated water column salinity being observed throughout this period and withV. vulnificusbeing nearly nonexistent. When salinities returned to normal after the drought abated in 2010, we were again able to routinely isolateV. vulnificusfrom the water column, although we were still unable to culture it from oysters. We suggest that the oysters were colonized with a more salt-tolerant bacterium during the drought, which displacedV. vulnificusand may be preventing recolonization.

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Section: Discussionmentioning

confidence: 99%

Apparent Loss of Vibrio vulnificus from North Carolina Oysters Coincides with a Drought-Induced Increase in Salinity

Froelich

Williams

Noble

et al. 2012

Appl Environ Microbiol

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“…However, depuration at low temperature of 15°C has been reported capable of reducing V. parahaemolyticus in the Crassostrea virginica by 2.1 and 2.9 log MPN/g, after 48 hours depuration in UV-treated depuration system [43]. Depuration with refrigerated seawater at 5°C reduced V. parahaemolyticus populations by >3.0 log MPN/g in the Pacific oysters Crassostrea gigas without significant fatality of the oysterswere demonstrated [38].…”

Section: Reduction Of Vibrio In Oystersmentioning

confidence: 99%

Evaluation of a Small Scale UV-treated Recirculating Depuration System for Oysters (Crassostrea iredalei)

Sorio¹,

Peralta²

2017

AJFST

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Oysters are filter-feeding organisms that tend to concentrate any suspended materials in its surrounding water including pathogenic bacteria. Since most oysters are eaten as raw or slightly cooked, they can act as vectors for pathogenic microorganisms and thus impose health risks to consumers. Depuration is one of the methods to reduce pathogenic bacteria in oysters to make it safe for sale and consumption. This study was designed to evaluate the effectiveness of the small scale UV-treated recirculating depuration system manipulating different parameters such as water flow rate and tank density. It aims to determine the effect of this UV-treated recirculating depuration system in reducing pathogenic bacteria in oysters such as E. coli, Salmonella, Vibrio cholera and Vibrio parahaemolyticus, and with the survival rate and meat yield of oysters. The experimental results showed that the depuration system was effective in reducing E. coli at different water flow rate (15L/min, 10L/min and 5L/min), in all density level (2 oysters/L, 4 oysters/L and 6 oysters/L). However, for Vibrio parahaemolyticus and Vibrio cholera reduction, only water flow rate of 15L/min at density level of 2 oysters/L and 4 oysters/L revealed to be effective. The survival of the oysters was high in treatments with a density of 2 oysters/ L. The meat yield revealed to have no significant difference (P<0.01) between treatments with water flow rates. In general, treatment with water flow rate of 15 L/min in combination with 2 oysters/L density showed most promise results on all analysis.

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“…The tdh gene was detected in four strains, and trh was detected in 23 strains (Figure 2). Several studies have suggested a strong influence of temperature on the concentration of vibrio in marine waters [27][28][29][30] . However, this study only established a correlation with V. vulnifi cus (p < 0.05).…”

Section: Seawater Parametersmentioning

confidence: 99%

Occurrence of potentially pathogenic Vibrio in oysters (Crassostrea gigas) and waters from bivalve mollusk cultivations in the South Bay of Santa Catarina

Ramos

Miotto

et al. 2014

Rev. Soc. Bras. Med. Trop.

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Introduction:This research aimed to identify and quantify potentially pathogenic Vibrio from different cultivations of bivalve shellfi sh in the State of Santa Catarina, Brazil, and water regions in the South Bay, as well as correlate the incidence of these microorganisms with the physicochemical parameters of marine waters. Methods: Between October 2008 and March 2009, 60 oyster and seawater samples were collected from six regions of bivalve mollusk cultivation, and these samples were submitted for Vibrio counts. Results: Twenty-nine (48.3%) oyster samples were revealed to be contaminated with one or more Vibrio species. The Vibrio parahaemolyticus and Vibrio vulnifi cus counts in the samples ranged from < 0.5 log 10 Most Probable Number (MPN) g -1 to 2.3 log 10 MPN g -1 oyster and from < 0.5 log 10 MPN g -1 to 2.1 log 10 MPN g -1 oyster, respectively. Of the 60 seawater samples analyzed, 44 (73.3%) showed signs of contamination with one or more vibrio species. The counts of V. parahaemolyticus and V. vulnifi cus in the samples ranged from < 0.3 log 10 MPN·100mL -1 to 1.7 log 10 MPN·100mL -1 seawater and from < 0.3 log 10 MPN·100mL -1 to 2.0 log 10 MPN·100mL -1 seawater, respectively. A positive correlation between V. vulnifi cus counts and the seawater temperature as well as a negative correlation between the V. parahaemolyticus counts and salinity were observed. Conclusions:The results suggest the need to implement strategies to prevent vibrio diseases from being transmitted by the consumption of contaminated bivalve shellfi sh.

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Temperature Effects on the Depuration of Vibrio parahaemolyticus and Vibrio vulnificus from the American Oyster (Crassostrea virginica)

Cited by 62 publications

References 24 publications

Apparent Loss of Vibrio vulnificus from North Carolina Oysters Coincides with a Drought-Induced Increase in Salinity

Apparent Loss of Vibrio vulnificus from North Carolina Oysters Coincides with a Drought-Induced Increase in Salinity

Evaluation of a Small Scale UV-treated Recirculating Depuration System for Oysters (<i>Crassostrea iredalei</i>)

Occurrence of potentially pathogenic Vibrio in oysters (Crassostrea gigas) and waters from bivalve mollusk cultivations in the South Bay of Santa Catarina

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