“…For example, Ingraham et al (1983) indicated, for E. coli, that the calculated Tmi, value was 3.5 "C while the true determined minimum growth temperature was 8 "C. Equation (2) has been successfully applied by Ratkowsky et al (1982Ratkowsky et al ( , 1983 to many data sets (Mohr and Krawiec, 1980;Reichardt and Morita, 1982). The effect of temperature on lag phase of growth has also been described by the square root model (Chandler and McMeekin, 1985;Griffiths and Phillips, 1988;Smith, 1985), with k equal to the inverse of the fag time.…”