Temperature-inducible outer membrane protein of Yersinia pseudotuberculosis and Yersinia enterocolitica is associated with the virulence plasmid

Bölin, Ingrid; Norlander, Lena; Wolf‐Watz, Hans

doi:10.1128/iai.37.2.506-512.1982

Cited by 267 publications

(127 citation statements)

References 29 publications

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“…For instance, 140, 34, 29 and 26 kD proteins were found only in the envelopes of bacteria grown at 37°C in the absence of calcium. These findings are very similar to those previously reported from other laboratories Straley & Brubaker 1981;Bolin et al 1982;Portnoy et al 1983).…”

Section: Resultssupporting

confidence: 93%

“…4&44 MD, which encodes certain membrane proteins believed to be essential for pathogenicity (Gemski et al 1980;Ferber & Brubaker 1981;Straley & Brubaker 1981). These proteins are synthesized during prolonged growth at 37°C but not if 2.5 mn~ol/l of calcium is added to the medium or if the organisms are grown at 28°C (Straley & Brubaker 1981;Bolin et al 1982;Martinez 1983). It has been postulated that temperature and calcium act as signals for the expression of these pathogenic traits.…”

mentioning

confidence: 99%

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Influence of nutrient‐limited growth on pathogenesis‐associated outer membrane proteins of Yersinia enterocolitica

Harakeh

Matin

1989

Journal of Applied Bacteriology

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From studies based on batch culture, it has been postulated that the expression of the virulence-associated proteins of Yersinia spp. is controlled by temperature and Ca2+, such that these proteins are synthesized only at the higher temperature (37 degrees C) and calcium-scarce conditions of the intracellular environment. It was found, however, that in Yersinia enterocolitica one of these proteins (140 kDa) is not synthesized at submaximal growth rates under any of the relevant conditions, and that another of the implicated proteins (34 kDa), is synthesized even at 28 degrees C during nutrient-limited growth. Thus, temperature and Ca2+ influence the synthesis of these proteins differently under growth conditions that better approximate the natural environments than do batch cultures.

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Section: Resultssupporting

confidence: 93%

mentioning

confidence: 99%

Influence of nutrient‐limited growth on pathogenesis‐associated outer membrane proteins of Yersinia enterocolitica

Harakeh

Matin

1989

Journal of Applied Bacteriology

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“…Through their ability to promote interactions with professional phagocytes as well as epithelial cells, the outer membrane adhesins invasin and YadA have the potential to contribute to both the establishment and clearance of Yersinia infections (Wiedemann et al ., 2001;Grosdent et al ., 2002). Both of these molecules are regulated at least in part by temperature; invasin is expressed to a greater degree at 28 ∞ C as compared with 37 ∞ C, whereas the reverse is true for YadA (Bolin et al ., 1982;Cornelis et al ., 1989;Revell and Miller, 2000;Nagel et al ., 2001). This regulation, which is expected to result in simultaneous expression of both adhesins at times during infection, is consistent with a role for invasin in the establishment of infection and for YadA following entry into the host.…”

Section: Introductionmentioning

confidence: 99%

Distinct mechanisms of integrin binding by Yersinia pseudotuberculosis adhesins determine the phagocytic response of host macrophages

Hudson

Bliska

Bouton

2005

Cellular Microbiology

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SummaryThe enteropathogenic yersiniae express two outer membrane adhesins, invasin and YadA, that contribute to pathogenesis. While invasin binds directly to b b b b 1 integrin receptors with high affinity, YadA binds indirectly through extracellular matrix (ECM) components. In this study, Yersinia pseudotuberculosis inv and yadA mutants were used to investigate how these distinct binding mechanisms compare and potentially compete in activating signalling pathways and promoting bacterial uptake by host macrophages. The efficiency of adhesin-mediated phagocytic responses was found to be dependent on the relative expression of invasin and YadA on the bacterial surface as well as the expression of ECM proteins in the extracellular milieu. Under conditions of low concentrations of ECM, invasin was found to be the dominant adhesin, promoting high levels of phagocytosis coincident with robust and sustained activation of the protein tyrosine kinases Fak and Pyk2, phosphorylation of the adaptor molecule Cas and activation of the small GTPase Rac1. In the presence of higher concentrations of ECM, YadA became the dominant functional adhesin through its ability to engage integrin receptors via an ECM bridge. We propose a model whereby invasin promotes robust and prolonged activation of phagocytic signalling cascades by inducing a 'highaffinity' integrin conformation as well as integrin clustering. We postulate that YadA-ECM promotes phagocytosis through a more transient activation of signalling cascades that arises from integrin clustering in the context of a cross-linked fibrillar ECM network.

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“…Purified Pchlide was used to inactivate five bacterial species. (Bolin et al, 1982) was a kind gift from Dr Petra Dersch, TU Braunschweig; and Escherichia coli DH10B was from Invitrogen.…”

Section: Introductionmentioning

confidence: 99%

Protochlorophyllide: a new photosensitizer for the photodynamic inactivation of Gram-positive and Gram-negative bacteria

Walther

Bröcker

Wätzlich

et al. 2008

FEMS Microbiology Letters

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The growing resistance against antibiotics demands the search for alternative treatment strategies. Photodynamic therapy is a promising candidate. The natural intermediate of chlorophyll biosynthesis, protochlorophyllide, was produced, purified and tested as a novel photosensitizer for the inactivation of five model organisms including Staphylococcus aureus, Listeria monocytogenes and Yersinia pseudotuberculosis, all responsible for serious clinical infections. When microorganisms were exposed to white light from a tungsten filament lamp (0.1 mW cm(-2)), Gram-positive S. aureus, L. monocytogenes and Bacillus subtilis were photochemically inactivated at concentrations of 0.5 mg L(-1) protochlorophyllide. Transmission electron microscopy revealed a disordered septum formation during cell division and the partial loss of the cytoplasmic cell contents. Gram-negative Y. pseudotuberculosis and Escherichia coli were found to be insensitive to protochlorophyllide treatment due to the permeability barrier of the outer membrane. However, the two bacteria were rendered susceptible to eradication by protochlorophyllide (10 mg L(-1)) upon addition of polymyxin B nonapeptide at 50 and 20 mg L(-1), respectively. The release of DNA and a detrimental rearrangement of the cytoplasm were observed.

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Temperature-inducible outer membrane protein of Yersinia pseudotuberculosis and Yersinia enterocolitica is associated with the virulence plasmid

Cited by 267 publications

References 29 publications

Influence of nutrient‐limited growth on pathogenesis‐associated outer membrane proteins of Yersinia enterocolitica

Influence of nutrient‐limited growth on pathogenesis‐associated outer membrane proteins of Yersinia enterocolitica

Distinct mechanisms of integrin binding by Yersinia pseudotuberculosis adhesins determine the phagocytic response of host macrophages

Protochlorophyllide: a new photosensitizer for the photodynamic inactivation of Gram-positive and Gram-negative bacteria

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