Temperature Induction Response (TIR) - A novel physiological approach for thermotolerant genotypes in chickpea (Cicer arietinum L.)

Abstract

<span>Chickpea (Cicer arietinum L.) is an important food legume cultivated in arid and semi-arid regions of the world. It is known that chickpea thrives well under drought prone condition. However, heat stress during reproductive development can cause significant yield loss and there is a greater variability for yield performance of chickpea under heat stress. It is extremely important to develop screening tools for identification of thermotolerant chickpea genotypes in view of increase in average global temperatures. In this context, a lab experiment was conducted to standardize the temperature induction response (TIR) protocol for chickpea seedlings using WGC-450 programmable plant growth chamber. Temperatures were standardized as sub lethal i.e. challenging temperatures as 38-480C (for 4 hours &30 min) and lethal temperatures as 500C (for 3 hours). This technique can be used as a potential tool to identify and select temperature tolerant lines at the seedling stage from a large population. A set of diverse chickpea germplasm comprising of 57 genotypes were screened for intrinsic tolerance using the standardized Thermo Induced Response (TIR) protocol. Among the genotypes NBeG-528,NBeG-458,NBeG-511,NBeG-177,NBeG-747,NBeG-732 and VIHAR showed highest thermo tolerance in terms of higher seedling survival with no reduction in root and shoot growth. The genotypes with intrinsic heat tolerance can be explored for the development of varieties suitable for late sown conditions in Andhra Pradesh where chickpea is prone to terminal heat stress.</span>

Effect of Temperature induction response on Cell viability, Cell Survivability, Malondialdehyde content and total soluble protein content of cotton (Gossypium hirsutum L.) genotypes

“…Percentage of seedlings survivability was declined when the plants are subjected to continuous stress period. Similarly, this trend was shown in some crops like cotton (Kheir et al, 2012), rice (Vijayalakshmi et al, 2015), maize (Dar et al, 2016) and chickpea (Raghavendra et al, 2017).…”

TIR approach and stress tolerance indices to identify donor for high-temperature stress tolerance in pepper (Capsicum annuum L.)

“…(2013) and Raghavendra et al . (2017) also advocated for the use of TIR technique to identify the tolerant and susceptible genotypes based on seedling survivability in ragi and chick pea, respectively.…”

“…This is the basis for TIR study and is a non-destructive method of screening a population like segregating, open pollinated, germplasm or mutated population. Venkatesh Babu et al (2013) and Raghavendra et al (2017) also advocated for the use of TIR technique to identify the tolerant and susceptible genotypes based on seedling survivability in ragi and chick pea, respectively. In the TIR study, the optimization of induction and challenging temperatures is the pre-requisite for a crop species.…”

A Study about Temperature Induction Response (TIR): Novel Physiological Approach for Thermotolerant Genotypes in Chickpea (Cicer arietinum L.)

“…After the recovery period of 48 hrs, the number of survived seedlings were counted in all the three treatments and used in calculation of per cent seedling survival [7].…”

“…After the recovery period of 48 hrs, the per cent reduction in root growth was calculated by using the formula [7,9].…”