Temperature-Insensitive Label-Free Sensors for Human IgG Based on S-Tapered Optical Fiber Sensors

Liu, Haowei; Sun, Yong; Guo, Jun; Liu, Wei; Liu, Le; Meng, Yansong; Yu, Xin

doi:10.1109/access.2021.3106343

Cited by 16 publications

(8 citation statements)

References 34 publications

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“…LoD values reported for conventional ELISA methods for IgG quantification range from 1.6 ng mL −1[ 67 ] down to 0.24 ng mL −1 , as claimed in a commercial kit. [ 68 ] More recently, a variety of different platforms for human‐IgG detection have been reported, such as molecular imprinted SPR chips [ 69 ] or optical sensors, [ 70,71 ] achieving LoDs of 56, [ 69 ] 37, [ 70 ] and 28 ng mL −1 . [ 71 ]…”

Section: Resultsmentioning

confidence: 99%

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An Artificial Miniaturized Peroxidase for Signal Amplification in Lateral Flow Immunoassays

Renzi

Piper

Nastri

et al. 2023

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Signal amplification strategies are widely used for improving the sensitivity of lateral flow immunoassays (LFiAs). Herein, the artificial miniaturized peroxidase Fe(III)‐MimochromeVI*a (FeMC6*a), immobilized on gold nanoparticles (AuNPs), is used as a strategy to obtain catalytic signal amplification in sandwich immunoassays on lateral flow strips. The assay scheme uses AuNPs decorated with the mini‐peroxidase FeMC6*a and anti‐human‐IgG as a detection antibody (dAb), for the detection of human‐IgG, as a model analyte. Recognition of the analyte by the capture and detection antibodies is first evidenced by the appearance of a red color in the test line (TL), due to the accumulation of AuNPs. Subsequent addition of 3,3′,5,5′‐tetramethylbenzidine (TMB) induces an increase of the test line color, due to the TMB being converted into an insoluble colored product, catalyzed by FeMC6*a. This work shows that FeMC6*a acts as an efficient catalyst in paper, increasing the sensitivity of an LFiA up to four times with respect to a conventional LFiA. Furthermore, FeMC6*a achieves lower limits of detection that are found in control experiments where it is replaced with horseradish peroxidase (HRP), its natural counterpart. This study represents a significant proof‐of‐concept for the development of more sensitive LFiAs, for different analytes, based on properly designed artificial metalloenzymes.

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Section: Resultsmentioning

confidence: 99%

“…[68] More recently, a variety of different platforms for human-IgG detection have been reported, such as molecular imprinted SPR chips [69] or optical sensors, [70,71] achieving LoDs of 56, [69] 37, [70] and 28 ng mL −1 . [71]…”

Section: Performance Of the Lfia: Comparison With A Conventional Assa...mentioning

confidence: 99%

An Artificial Miniaturized Peroxidase for Signal Amplification in Lateral Flow Immunoassays

Renzi

Piper

Nastri

et al. 2023

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“…We compare the IgG sensing sensitivity and anti-environmental interference capability of the proposed HIPFG immunosensor to other reported optical fiber sensors in Table 2 . Although previous reports on IgG sensors are superior in LOD for IgG detection, most of them lack evaluation for environmental factors [ 40 , 41 ], and some of them are sensitive to those factors, which limits their application in mobile equipment and compact devices [ 24 ]. As listed in Table 2 , the sensitivities for torsion, strain, and temperature of HIPFG are significantly smaller than the corresponding values of the previously reported optical fiber IgG sensor, providing stable and reliable sensing performance for IgG sensing.…”

Section: Resultsmentioning

confidence: 99%

All Fiber-Optic Immunosensors Based on Elliptical Core Helical Intermediate-Period Fiber Grating with Low-Sensitivity to Environmental Disturbances

et al. 2022

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An all fiber-optic immunosensor based on elliptical core helical intermediate-period fiber grating (E-HIPFG) is proposed for the specific detection of human immunoglobulin G (human IgG). E-HIPFGs are all-fiber transducers that do not include any additional coating materials or fiber architectures, simplifying the fabrication process and promising the stability of the E-HIPFG biosensor. For human IgG recognition, the surface of an E-HIPFG is functionalized by goat anti-human IgG. The functionalized E-HIPFG is tested by human IgG solutions with a concentration range of 10–100 μg/mL and shows a high sensitivity of 0.018 nm/(μg/mL) and a limit of detection (LOD) of 4.7 μg/mL. Notably, the functionalized E-HIPFG biosensor is found to be insensitive to environmental disturbances, with a temperature sensitivity of 2.6 pm/°C, a strain sensitivity of 1.2 pm/με, and a torsion sensitivity of −23.566 nm/(rad/mm). The results demonstrate the considerable properties of the immunosensor, with high resistance to environmental perturbations, indicating significant potential for applications in mobile biosensors and compact devices.

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“…The high-power evanescent field has the ability to detect subtle changes on the fiber surface, including biomolecules, temperature, pressure, chemical ions, and gases. This results in a higher level of sensitivity and an improved limit of detection (LOD) for the sensor [ 32 , 33 , 34 , 35 ]. As a result, optical fiber sensors with TOF structures have numerous applications in a variety of domains [ 36 , 37 ].…”

Section: Introductionmentioning

confidence: 99%

Advances in Tapered Optical Fiber Sensor Structures: From Conventional to Novel and Emerging

et al. 2023

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Optical fiber sensors based on tapered optical fiber (TOF) structure have attracted a considerable amount of attention from researchers due to the advantages of simple fabrication, high stability, and diverse structures, and have great potential for applications in many fields such as physics, chemistry, and biology. Compared with conventional optical fibers, TOF with their unique structural characteristics significantly improves the sensitivity and response speed of fiber-optic sensors and broadens the application range. This review presents an overview of the latest research status and characteristics of fiber-optic sensors and TOF sensors. Then, the working principle of TOF sensors, fabrication schemes of TOF structures, novel TOF structures in recent years, and the growing emerging application areas are described. Finally, the development trends and challenges of TOF sensors are prospected. The objective of this review is to convey novel perspectives and strategies for the performance optimization and design of TOF sensors based on fiber-optic sensing technologies.

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Temperature-Insensitive Label-Free Sensors for Human IgG Based on S-Tapered Optical Fiber Sensors

Cited by 16 publications

References 34 publications

An Artificial Miniaturized Peroxidase for Signal Amplification in Lateral Flow Immunoassays

An Artificial Miniaturized Peroxidase for Signal Amplification in Lateral Flow Immunoassays

All Fiber-Optic Immunosensors Based on Elliptical Core Helical Intermediate-Period Fiber Grating with Low-Sensitivity to Environmental Disturbances

Advances in Tapered Optical Fiber Sensor Structures: From Conventional to Novel and Emerging

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