The non-Bragg defect mode (NBDM) of elastic surface waves is experimentally investigated by inserting a defect in the middle of an antisymmetric periodic corrugated aluminum plate, which has been known as the non-Bragg structures since the observed band gaps are different from the traditional Bragg ones. Generally, the non-Bragg band gaps, existing away from the Bragg ones in a perfectly periodic waveguide, are created by the resonances of different transverse guided modes. The transmission spectra of elastic surface waves in antisymmetric structures with defects reveal the presence of defect modes within the non-Bragg gaps. Notably, the NBDM exhibits significant distribution characteristics in comparison to the traditional Bragg defect mode, including more complex elastic wave higher-order modes and localized wave energy near the defect. Consequently, the NBDM observed in the antisymmetric periodic waveguide with defects holds potential for utilization in other elastic wave functional devices, including filters and wave intensifiers.