Temperature range for formic hydrogenlyase induction and activity in psychrophilic and mesophilic bacteria

Quist, R. G.; Stokes, J. L.

doi:10.1007/bf02219109

Cited by 8 publications

(3 citation statements)

References 21 publications

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“…E. coli possesses two inducible, soluble, membrane-associated formate dehydrogenase complexes (24). One of these complexes is involved in the evolution of carbon dioxide and molecular hydrogen from formate, and it is functional only under anaerobic growth conditions (9,22). If formate dehydrogenase is a target of the inhibitory effect of the elevated temperature upon nonfecal coliforms, the lack of gas production in EC broth at 44.5°C would be expected, and this was observed.…”

Section: Resultsmentioning

confidence: 99%

Fecal coliform elevated-temperature test: a physiological basis

Dockins¹,

McFeters²

1978

Appl Environ Microbiol

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The physiological basis of the Eijkman elevated-temperature test for differentiating fecal from nonfecal coliforms was investigated. Manometric studies indicated that the inhibitory effect upon growth and metabolism in a nonfecal coliform at 44.50C involved cellular components common to both aerobic and fermentative metabolism of lactose. Radioactive substrate incorporation experiments implicated cell membrane function as a principal focus for temperature sensitivity at 44.5°C. A temperature increase from 35 to 44.50C drastically reduced the rates of [14C]glucose uptake in nonfecal coliforms, whereas those of fecal coliforms were essentially unchanged. In addition, relatively low levels of nonfecal coliform ,B-galactosidase activity coupled with thermal inactivation ofthis enzyme at a comparatively low temperature may also inhibit growth and metabolism of nonfecal coliforms at the elevated temperature.

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Section: Resultsmentioning

confidence: 99%

Fecal coliform elevated-temperature test: a physiological basis

Dockins¹,

McFeters²

1978

Appl Environ Microbiol

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“…The activity of induced formic hydrogenlyase was determined in a Warburg respirometer (Aminco) by the technique of Quist and Stokes (21). Duplicate vessels contained 2.0 ml of a washed cell suspenstion in the main compartment, and 0.2 ml of a 20% KOH solution in the center well for the absorption of CO2.…”

Section: Methodsmentioning

confidence: 99%

Formic Hydrogenlyase Induction as a Basis for the Eijkman Fecal Coliform Concept

Hendricks¹

1970

Applied Microbiology

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Many Enterobacter species recovered from a relatively unpolluted freshwater stream had the capacity to produce gas from glucose, lactose, and formate at 44.5 C. Composition of the evolved gas was shown to be CO2 and H2, which suggested that the enzyme system formic hydrogenlyase functions as the basis for the Eijkman fecal coliform concept. It is postulated that there are at least two different and distinct biochemical types of Enterobacter in the natural aquatic environment: one that lacks an active formic hydrogenlyase at 44.5 C and is associated with the intestinal tract of man and certain animals, and another which possesses the active enzyme at the elevated temperature and is found in soil or on vegetation free from fecal pollution.

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“…Many investigators have examined marine habitats for the occurrence of thermosensitive microorganisms and often with varied results (1). ZoBell and Conn (8) and others (3,5,6) have shown that increased recovery rates of these organisms are often possible if the temperature of the primary isolation media is lowered, and numerous studies have been made demonstrating the extreme sensitivity of psychrophiles (1,5,6) and their enzyme systems (2,4,6) to temperatures of 15 C and above.…”

mentioning

confidence: 99%