Temperature Relations for Seed Germination Potential and Seedling Vigor in Palmarosa (<i>Cymbopogon martinii</i>)

Kumar, Birendra; Verma, Sanjeet K.; Ram, Govind; Singh, Harminder Pal

doi:10.1080/15427528.2012.689799

Cited by 29 publications

(9 citation statements)

References 8 publications

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“…Seedling vigour index I and vigour index II were calculated according to Kumar et al. (2012) as follows:

…”

Section: Methodsmentioning

confidence: 99%

Cyto-genotoxic potential of petroleum refinery wastewater mixed with domestic sewage used for irrigation of food crops in the vicinity of an oil refinery

Khatoon¹,

Malik²

2021

Heliyon

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Petroleum refinery wastewater combined with domestic sewage were collected from the open channel in the vicinity of Mathura oil refinery, UP (India) and analysed by inductively coupled plasma optical emission spectrometry (ICP-OES) and gas chromatography-mass spectrometry (GC-MS) for elemental analysis and organic pollutants, respectively. Several potentially toxic and non-toxic elements were found to be present in the wastewater samples. GC-MS analysis revealed the presence of several organic contaminants including pesticides. Wastewater samples were extracted using amberlite XAD4/8 resins and liquid-liquid extraction procedures using different organic solvents. The extracts were tested for their cyto-genotoxic potential using bacterial (Salmonella mutagenicity test, E. coli K-12 DNA repair defective mutants, Bacteriophage λ assay) and plant (Vigna mungo phytotoxicity test, Allium cepa chromosomal aberration assay) systems. A significant increase was observed in the number of revertants of TA97a, TA98 and TA100 strains with the test samples and XAD concentrated samples were found to be more mutagenic than liquid-liquid extracts. Colony forming units (CFUs) of DNA repair defective mutants of E. coli K-12 recA, lexA and polA declined significantly as compared to their isogenic wild-type counterparts with the test samples. Significant reduction in plaque forming units (PFUs) of bacteriophage λ was also found on treatment with the solvent extracts. Presence of several toxic pollutants in the wastewater apply prohibitive action on the seed germination process. Germination rate of Vigna mungo seeds as well as radicle and plumule lengths were found to be affected when treated with different concentration of wastewater as compared to control. Present study also indicated concentration dependent reduction in mitotic index of A. cepa i.e., 16.38% at 5% and 9.74% at 100% wastewater and percentage of aberrant cells were highest at 100% effluent. Present findings indicated that mutagenicity/genotoxicity of wastewater is due to the mixture of genotoxins; poses serious hazards to the receiving waterbodies which require continuous monitoring and remedial measures for their improvement.

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“…Seedling vigour index I and vigour index II were calculated according to Kumar et al. (2012) as follows:

…”

Section: Methodsmentioning

confidence: 99%

Cyto-genotoxic potential of petroleum refinery wastewater mixed with domestic sewage used for irrigation of food crops in the vicinity of an oil refinery

Khatoon¹,

Malik²

2021

Heliyon

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“…The values were put in the formula and multipliedwith seed germination percentage. The formula is as follows [55].…”

Section: Mean Germination Rate (Mgr)mentioning

confidence: 99%

Using Halothermal Time Model to Describe Barley (Hordeumvulgare L.) Seed Germination Response to Water Potential and Temperature

Ullah

Sadaf

Ullah

et al. 2022

Life

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Barley (Hordeum vulgare L.) is a salt-tolerant crop with considerable economic value in salinity-affected arid and semiarid areas. In the laboratory experiment, the halothermal time (HaloTT) model was used to examine barley seed germination (SG) at six constant cardinal temperatures (Ts) of 15, 20, 25, 30, 35, and 40 °C under five different water potentials (ψs) of 0, −0.5, −1.5, −1.0, and −2.0 MPa. Results showed that at optimum moisture (0 MPa), the highest germination percentage (GP) was recorded at 20 °C and the lowest at 40 °C. Moreover, GP increased with the accelerated aging period (AAP) and significantly (p ≤ 0.05) decreased with high T. In addition, with a decrease of ψ from 0 to −0.5, −1, 1.5, and −2.0 MPa, GP decreased by 93.33, 76.67, 46.67, and 33.33%, respectively, in comparison with 0 MPa. The maximum halftime constant (θHalo) and coefficient of determination (R2) values were recorded at 20 °C and 30 °C, respectively. The optimum temperature (To) for barley is 20 °C, base Ψ of 50th percentile (Ψb (50)) is −0.23 Mpa, and standard deviation of Ψb (σΨb) is 0.21 MPa. The cardinal Ts for germination is 15 °C (Tb), 20 °C (To), and 40 °C (Tc). The GP, germination rate index (GRI), germination index (GI), coefficient of the velocity of germination (CVG), germination energy (GE), seed vigor index I and II (SVI-I & II), Timson germination index (GI), and root shoot ratio (RSR) were recorded maximum at 0 MPa at 20 °C and minimum at −2.0 MPa at 40 °C. Mean germination time (MGT) and time to 50% germination (T 50%) were maximum at −2 MPa at 40 °C, and minimum at 20 °C, respectively. In conclusion, the HaloTT model accurately predicted the germination time course of barley in response to T, Ψ, or NaCl. Therefore, barley can be regarded as a salt-tolerant plant and suitable for cultivation in arid and semi-arid regions due to its high resistance to salinity.

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“…After 30 days, destructive sampling of the seedlings was done. Seedlings were analyzed for growth parameters, viz., fresh weight, and shoot length to calculate germination percentage (GP) and seedling vigor index using the following equations [27] Assay for wilt disease control with cake-based formulation…”

Section: Seed Germination Study With Cake-based Formulationmentioning

confidence: 99%

Synergistic effect of oilseed cake and biocontrol agent in the suppression of Fusarium wilt in Solanum lycopersicum

Jangir

Sharma

2020

Braz J Microbiol

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The antagonistic efficacy of a biocontrol agent in combination with oilseed cake against fungal phytopathogens has been sparsely explored. The present study aimed to evaluate the antifungal activity of a biocontrol agent (Trichoderma harzianum MTCC 3928) formulated with oilseed cake (OSC) against Fusarium oxysporum f. sp. lycopersici responsible for causing vascular wilt in Solanum lycopersicum. In in vitro studies, OSC of mustard (Brassica juncea) exhibited significant mycelial inhibition against the pathogen. The volatile plate assay showed mycelial inhibition of 70 and 40% with unautoclaved and autoclaved mustard cakes, respectively. The aqueous extract (10% v/v) of the mustard cake was the most effective with 47.3% mycelial inhibition of pathogen over control. In addition, volatiles and aqueous extract of mustard cake subjected to GC-MS analysis revealed a range of antifungal bioactive compounds with hexanedioic acid, dioctyl ester (16.57%), and oleic acid trimethylsilyl ester (12.41%) being predominant compounds. In in vitro studies, it was noticed that the T. harzianum strain was compatible with mustard cake, and hence used as a growth substrate for its mass multiplication. SEM analysis revealed no distortion in spores and mycelium of T. harzianum grown on the mustard cake. Further, seed germination assay suggested the optimum concentration of mustard cake (10%) supporting the germination rate and economics of formulation development. In in planta assay, the combination of biocontrol agent and mustard cake showed 48% disease reduction, and~40% with T. harzianum alone in comparison to untreated control. Also, the combination of mustard cake and T. harzianum significantly enhanced the growth parameters of S. lycopersicum. The findings of the current study identified an environmentally friendly alternative for mitigation of Fusarium wilt, thereby providing a sustainable option for mitigation of wilt disease and enhancement of plant health.

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Temperature Relations for Seed Germination Potential and Seedling Vigor in Palmarosa (Cymbopogon martinii)

Cited by 29 publications

References 8 publications

Cyto-genotoxic potential of petroleum refinery wastewater mixed with domestic sewage used for irrigation of food crops in the vicinity of an oil refinery

Cyto-genotoxic potential of petroleum refinery wastewater mixed with domestic sewage used for irrigation of food crops in the vicinity of an oil refinery

Using Halothermal Time Model to Describe Barley (Hordeumvulgare L.) Seed Germination Response to Water Potential and Temperature

Synergistic effect of oilseed cake and biocontrol agent in the suppression of Fusarium wilt in Solanum lycopersicum

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