Temperature-responsive Smart Packing Materials Utilizing Multi-functional Polymers

Ayano, Eri; Kanazawa, Hideko

doi:10.2116/analsci.30.167

Cited by 20 publications

(10 citation statements)

References 49 publications

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“…Whereas conventional chromatography uses reverse-phase columns, in which the partitioning is controlled by the mobile phase composition, in our temperature-responsive chromatography system, the partitioning is controlled by external temperature changes and water alone is used as the mobile phase. [9][10][11] The LCST of PNIPAAm can be adjusted to a specific temperature through its copolymer composition. 12,13 The addition of butyl methacrylate (BMA) as a hydrophobic monomer lowers the LCST.…”

Section: Introductionmentioning

confidence: 99%

Temperature-responsive molecular recognition chromatography using phenylalanine and tryptophan derived polymer modified silica beads

Hiruta

Kanazashi

Ayano

et al. 2016

Analyst

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Temperature-responsive polymers incorporating molecular-recognition sites were developed as stationary phases for high-performance liquid chromatography (HPLC). The grafted stationary phases consisted of functional copolymers composed of N-isopropylacrylamide (NIPAAm) and N-acryloyl aromatic amino acid methyl esters, i.e., phenylalanine and tryptophan methyl esters (Phe-OMe and Trp-OMe). Three novel temperature-responsive polymers, P(NIPAAm-co-Phe-OMe5), P(NIPAAm-co-Phe-OMe10), and P(NIPAAm-co-Trp-OMe5), were synthesized. These copolymers exhibited a reversible hydrophilic/hydrophobic phase transition at their lower critical solution temperatures (LCSTs). The polymers were grafted onto aminopropyl silica using an activated ester-amine coupling method, and were packed into a stainless steel column, which was connected to an HPLC system. Temperature-responsive chromatography was conducted using water as the sole mobile phase. More hydrophobic analytes were retained longer, and the retention times of aromatic steroids and aromatic amino acids were dramatically increased. This indicated that π-π interactions occurred between the phenyl or indole moieties of phenylalanine or tryptophan, respectively, and the aromatic compounds. Furthermore, the retention times of compounds with hydrogen bond acceptors were higher with P(NIPAAm-co-Trp-OMe5), which contained indole as a hydrogen bond donor, than with P(NIPAAm-co-Phe-OMe5). This indicated that hydrogen bonding occurred between the stationary phase and the analytes. These results indicate that hydrophobic, π-π, and hydrogen bonding interactions all affected the separation mode of the temperature-responsive chromatography, and led to selective separation with molecular recognition. Both temperature-response and molecular recognition characteristics are present in the proposed separation system that utilizes a temperature-responsive polymer bearing aromatic amino acid derivatives.

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Section: Introductionmentioning

confidence: 99%

Temperature-responsive molecular recognition chromatography using phenylalanine and tryptophan derived polymer modified silica beads

Hiruta

Kanazashi

Ayano

et al. 2016

Analyst

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“…Furthermore, the addition of stimuli-responsive (pH [5], ions [6], saccharides [7], and light [8]) moieties in the copolymer composition causes a phase transition of PNIPAm-based polymers in response to stimuli. Therefore, PNIPAm-based stimuliresponsive polymers have been widely used in biomaterials science and technology for applications such as drug delivery systems [9], cell culture substrates [10], and separation systems [11].…”

Section: Introductionmentioning

confidence: 99%

The effects of anionic electrolytes and human serum albumin on the LCST of poly( N -isopropylacrylamide)-based temperature-responsive copolymers

Hiruta

Nagumo

Suzuki

et al. 2015

Colloids and Surfaces B: Biointerfaces

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“…In particular, the transformation principle of thermo-responsive hydrogels is based on their wettability and solubility alteration following the change of temperature [92,93]. Temperature responsiveness is probably one of the most commonly investigated mechanisms to achieve user-defined functionality [94,95]. Hu and colleagues provided a basic idea to fabricate a double-network hydrogel that combined two components with drastically different thermal properties induced by crystallization of one component [93,[96][97][98].…”

Section: Temperature-responsive Materialsmentioning

confidence: 99%

4D bioprinting: the next-generation technology for biofabrication enabled by stimuli-responsive materials

et al. 2016

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Four-dimensional (4D) bioprinting, encompassing a wide range of disciplines including bioengineering, materials science, chemistry, and computer sciences, is emerging as the next-generation biofabrication technology. By utilizing stimuli-responsive materials and advanced three-dimensional (3D) bioprinting strategies, 4D bioprinting aims to create dynamic 3D patterned biological structures that can transform their shapes or behavior under various stimuli. In this review, we highlight the potential use of various stimuli-responsive materials for 4D printing and their extension into biofabrication. We first discuss the state of the art and limitations associated with current 3D printing modalities and their transition into the inclusion of the additional time dimension. We then suggest the potential use of different stimuli-responsive biomaterials as the bioink that may achieve 4D bioprinting where transformation of fabricated biological constructs can be realized. We finally conclude with future perspectives.

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Temperature-responsive Smart Packing Materials Utilizing Multi-functional Polymers

Cited by 20 publications

References 49 publications

Temperature-responsive molecular recognition chromatography using phenylalanine and tryptophan derived polymer modified silica beads

Temperature-responsive molecular recognition chromatography using phenylalanine and tryptophan derived polymer modified silica beads

The effects of anionic electrolytes and human serum albumin on the LCST of poly( N -isopropylacrylamide)-based temperature-responsive copolymers

4D bioprinting: the next-generation technology for biofabrication enabled by stimuli-responsive materials

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