Temperature-responsive Solid-phase Extraction Column for Biological Sample Pretreatment

Akimaru, Michiko; Okubo, Kohei; Hiruta, Yuki; Kanazawa, Hideko

doi:10.2116/analsci.31.881

Cited by 20 publications

(11 citation statements)

References 19 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Whereas conventional chromatography uses reverse-phase columns, in which the partitioning is controlled by the mobile phase composition, in our temperature-responsive chromatography system, the partitioning is controlled by external temperature changes and water alone is used as the mobile phase. [9][10][11] The LCST of PNIPAAm can be adjusted to a specific temperature through its copolymer composition. 12,13 The addition of butyl methacrylate (BMA) as a hydrophobic monomer lowers the LCST.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Temperature-responsive molecular recognition chromatography using phenylalanine and tryptophan derived polymer modified silica beads

Hiruta

Kanazashi

Ayano

et al. 2016

Analyst

Self Cite

View full text Add to dashboard Cite

Temperature-responsive polymers incorporating molecular-recognition sites were developed as stationary phases for high-performance liquid chromatography (HPLC). The grafted stationary phases consisted of functional copolymers composed of N-isopropylacrylamide (NIPAAm) and N-acryloyl aromatic amino acid methyl esters, i.e., phenylalanine and tryptophan methyl esters (Phe-OMe and Trp-OMe). Three novel temperature-responsive polymers, P(NIPAAm-co-Phe-OMe5), P(NIPAAm-co-Phe-OMe10), and P(NIPAAm-co-Trp-OMe5), were synthesized. These copolymers exhibited a reversible hydrophilic/hydrophobic phase transition at their lower critical solution temperatures (LCSTs). The polymers were grafted onto aminopropyl silica using an activated ester-amine coupling method, and were packed into a stainless steel column, which was connected to an HPLC system. Temperature-responsive chromatography was conducted using water as the sole mobile phase. More hydrophobic analytes were retained longer, and the retention times of aromatic steroids and aromatic amino acids were dramatically increased. This indicated that π-π interactions occurred between the phenyl or indole moieties of phenylalanine or tryptophan, respectively, and the aromatic compounds. Furthermore, the retention times of compounds with hydrogen bond acceptors were higher with P(NIPAAm-co-Trp-OMe5), which contained indole as a hydrogen bond donor, than with P(NIPAAm-co-Phe-OMe5). This indicated that hydrogen bonding occurred between the stationary phase and the analytes. These results indicate that hydrophobic, π-π, and hydrogen bonding interactions all affected the separation mode of the temperature-responsive chromatography, and led to selective separation with molecular recognition. Both temperature-response and molecular recognition characteristics are present in the proposed separation system that utilizes a temperature-responsive polymer bearing aromatic amino acid derivatives.

show abstract

Section: Introductionmentioning

confidence: 99%

“…16 It is possible to impart new separation modes to temperature-responsive chromatography systems by introducing functional monomers into PNIPAAm. [9][10][11] In this study, we focused on aromatic amino acids to introduce a new separation mode featuring molecular recognition. Aromatic amino acids have unique and important properties.…”

Section: Introductionmentioning

confidence: 99%

Temperature-responsive molecular recognition chromatography using phenylalanine and tryptophan derived polymer modified silica beads

Hiruta

Kanazashi

Ayano

et al. 2016

Analyst

Self Cite

View full text Add to dashboard Cite

show abstract

“…Temperature‐responsive polymers are smart materials that exhibit temperature‐regulated hydrophilic/hydrophobic characteristics. Poly( N ‐isopropylacrylamide) [P(NIPAAm)] is one of the most commonly used thermo‐responsive polymers and has been proved to be successful for SPE . In addition to particle‐packed column, PNIPAAm was also used to prepare monolithic column for SPE by our group .…”

Section: Introductionmentioning

confidence: 99%

“…Poly(N-isopropylacrylamide) [P(NIPAAm)] is one of the most commonly used thermo-responsive polymers and has been proved to be successful for SPE. 15,16 In addition to particle-packed column, PNIPAAm was also used to prepare monolithic column for SPE by our group. 17 Unfortunately, the monolith had not exhibited temperature-controlled properties.…”

Section: Introductionmentioning

confidence: 99%

Effect of different initiators on temperature‐sensitive monolithic columns and application in online enrichment of β‐sitosterol

Guo

Wang

Lan

et al. 2019

J of Applied Polymer Sci

View full text Add to dashboard Cite

Three temperature‐sensitive monolithic columns were prepared by atom transfer radical polymerization and oxidation–reduction method using the initiator systems of CCl4/FeCl2, BPO/DMA, and APS/TEMED, respectively. The three monoliths were characterized by scanning electron microscopy, nitrogen adsorption, and thermogravimetric analysis. The obtained monolithic columns were used as on‐line solid phase extraction sorbent coupled with high‐performance liquid chromatography for the enrichment of β‐sitosterol. By comparing some influencing factors on the adsorption, the optimum temperature‐sensitive monolithic column which was initiated by CCl4/FeCl2 was selected for enrichment of β‐sitosterol from plant oil. The maximum adsorption capacity of the monolith for β‐sitosterol was 10.0031 mg/g. The limit of detection and limit of quantitation were 0.039 and 0.13 mg/mL, respectively. The recovery was in the range of 90.21–98.26%. The results showed that the monolith had better selectivity for β‐sitosterol and could be used for enrichment of β‐sitosterol in food samples. © 2019 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2019, 136, 47683.

show abstract

“…Biological sample pretreatment is an important part of biopharmaceutical analysis and the most troublesome aspect in the entire process of sample separation and analysis. Compared with the raw materials needed for pharmaceutical preparations, biological samples are more complicated: a drug, often in trace quantities, can distribute in complex biological media, and a sample may contain a large number of endogenous substances including the metabolites of the assayed drug 1 , 2 . Thus, most biological samples, such as organs, tissues, plasma and urine, must be properly processed for separation, purification, enrichment and chemical modification to meet the requirements of the analytical instruments, such as high performance liquid chromatography (HPLC) and mass spectrometry (MS).…”

Section: Introductionmentioning

confidence: 99%

New techniques of on-line biological sample processing and their application in the field of biopharmaceutical analysis

Peng

Tang

Zhou

et al. 2016

Acta Pharmaceutica Sinica B

View full text Add to dashboard Cite

Biological sample pretreatment is an important step in biological sample analysis. Due to the diversity of biological matrices, the analysis of target substances in these samples presents significant challenges to sample processing. To meet these emerging demands on biopharmaceutical analysis, this paper summarizes several new techniques of on-line biological sample processing: solid phase extraction, solid phase micro-extraction, column switching, limited intake filler, molecularly imprinted solid phase extraction, tubular column, and micro-dialysis. We describe new developments, principles, and characteristics of these techniques, and the application of liquid chromatography–mass spectrometry (LC–MS) in biopharmaceutical analysis with these new techniques in on-line biological sample processing.

show abstract

Temperature-responsive Solid-phase Extraction Column for Biological Sample Pretreatment

Cited by 20 publications

References 19 publications

Temperature-responsive molecular recognition chromatography using phenylalanine and tryptophan derived polymer modified silica beads

Temperature-responsive molecular recognition chromatography using phenylalanine and tryptophan derived polymer modified silica beads

Effect of different initiators on temperature‐sensitive monolithic columns and application in online enrichment of β‐sitosterol

New techniques of on-line biological sample processing and their application in the field of biopharmaceutical analysis

Contact Info

Product

Resources

About