Temperature sensitivity of mineral-enzyme interactions on the hydrolysis of cellobiose and indican by β-glucosidase

Yang, Ziming; Liao, Yiju; Fu, Xuan; Zaporski, Jared; Peters, Stéphanie; Jamison, Megan N.; Liu, Yurong; Wullschleger, Stan D.; Graham, David E.; Gu, Baohua

doi:10.1016/j.scitotenv.2019.05.479

Cited by 24 publications

(23 citation statements)

References 49 publications

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“…The amount of BG desorption was negligible (<3%) (Figure S4b in Supporting Information ), indicating that BG adsorption to the minerals was irreversible (Liu et al., 2019; Gómez et al., 2010; Yang et al., 2019). Moreover, the steric effects and restricted transport in mesoporous mineral surfaces possibly impeded BG desorption (Table S1 in Supporting Information ; Mayer et al., 2004; Zimmerman et al., 2004).…”

Section: Resultsmentioning

confidence: 99%

“…Because BG activity depended on mass, it was thus normalized relative to its mass (Yang et al., 2019; Zang et al., 2020):

\text{BG}\,\text{Activity}\,(\text{nmol/hr/BG}\,\text{mg})=\frac{\text{Activity}}{\text{Mass}}

…”

Section: Methodsmentioning

confidence: 99%

“…One hour was sufficient to reach adsorption equilibrium (Tietjen & Wetzel, 2003). To study the desorption of BG from mineral surface, adsorbed BG was rinsed with acetate buffer for 1 hr (Yang et al., 2019; Zang et al., 2020).…”

Section: Methodsmentioning

confidence: 99%

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The Important Role of Enzyme Adsorbing Capacity of Soil Minerals in Regulating β‐Glucosidase Activity

Sheng

Dong

Coffin

et al. 2022

Geophysical Research Letters

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Mineral surfaces are known for their ability to adsorb enzymes, but their effects on enzyme activity are not well studied. This study explores the extent to which interaction with primary silicates and secondary minerals affects the activity and longevity of a model soil enzyme β‐glucosidase (BG). The BG activity was correlated with the capacity‐related properties of minerals (controlling space for adsorption), including specific surface area (SSA) and the SSA‐normalized amount of BG adsorption, but not with the functional properties (controlling the mechanisms of adsorption) such as surface charge. BG conformational change is a function of SSA‐normalized amount of adsorption, suggesting that spatially more distributed enzyme molecules would have a higher probability of encounter with the substrate than the congested molecules. The highest decay rate of BG activity was observed in the presence of Mn‐oxide. Our results shed new light on the effect of mineral surface on enzyme activity.

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Section: Resultsmentioning

confidence: 99%

“…Because BG activity depended on mass, it was thus normalized relative to its mass (Yang et al., 2019; Zang et al., 2020):

\text{BG}\,\text{Activity}\,(\text{nmol/hr/BG}\,\text{mg})=\frac{\text{Activity}}{\text{Mass}}

…”

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

The Important Role of Enzyme Adsorbing Capacity of Soil Minerals in Regulating β‐Glucosidase Activity

Sheng

Dong

Coffin

et al. 2022

Geophysical Research Letters

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“…Synergy between cellulolytic enzymes and these enzymes from Acremonium alcalophilum and Aspergillus nidulans has been described, which deserves more attention in the future [48,49]. The currently reported lignocellulosic degrading bacteria and fungi with industrial application prospects presented distinct optimal conditions for different enzymes even in the same induced enzyme system, and the stabilities against temperature and pH were also different, so it is necessary to determine the optimal conditions for enzymatic hydrolysis and saccharification with overall consideration [1,50,51]. In the present study, similar optimal temperature and pH for various functional enzymes in T. harzianum EM0925 enzyme cocktail were observed, which gave rise to better synergistic effects in efficient deconstruction of lignocellulose complex.…”

Section: Discussionmentioning

confidence: 99%

Low-Cost Cellulase-Hemicellulase Mixture Secreted by Trichoderma harzianum EM0925 with Complete Saccharification Efficacy of Lignocellulose

Zhang

Yang

Luo

et al. 2020

IJMS

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Fermentable sugars are important intermediate products in the conversion of lignocellulosic biomass to biofuels and other value-added bio-products. The main bottlenecks limiting the production of fermentable sugars from lignocellulosic biomass are the high cost and the low saccharification efficiency of degradation enzymes. Herein, we report the secretome of Trichoderma harzianum EM0925 under induction of lignocellulose. Numerously and quantitatively balanced cellulases and hemicellulases, especially high levels of glycosidases, could be secreted by T. harzianum EM0925. Compared with the commercial enzyme preparations, the T. harzianum EM0925 enzyme cocktail presented significantly higher lignocellulolytic enzyme activities and hydrolysis efficiency against lignocellulosic biomass. Moreover, 100% yields of glucose and xylose were obtained simultaneously from ultrafine grinding and alkali pretreated corn stover. These findings demonstrate a natural cellulases and hemicellulases mixture for complete conversion of biomass polysaccharide, suggesting T. harzianum EM0925 enzymes have great potential for industrial applications.

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“…Furthermore, enzymes have an optimum temperature where the efficiency of the reaction peaks. Temperature variation above or below this point can reduce the speed of the reaction until its complete inactivation (Yang et al, 2019;Gómez et al, 2020). Throughout their evolution, microorganisms have developed different biochemical strategies to maintain extracellular enzyme activity against environmental temperature changes.…”

Section: Ecology Of Soil Extracellular Enzymesmentioning

confidence: 99%

Contribution of enzymes to soil quality and the evolution of research in Brazil

Sobucki¹,

Ramos²,

Meireles³

et al. 2021

Revista Brasileira De Ciência Do Solo

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Extracellular soil enzymes are fundamental for the functioning of ecosystems. Several processes in the soil depend on the activity of these enzymes, including plant decomposition, soil organic matter formation/mineralization, and nutrient cycling. Moreover, extracellular enzyme activity occurs in the soil and is therefore influenced by environmental factors. Due to the high sensitivity to these factors, extracellular enzymes are used for monitoring soil quality. This review aimed to present the main contributions of soil enzymes to agriculture, emphasizing the dynamics of elements in the soil and the environmental factors that modulate enzyme activity. With this knowledge, the relationship of extracellular enzymes to soil quality is demonstrated and their use as a tool for soil monitoring. Finally, the evolution of research on soil enzymes in Brazil is presented, and the perspectives of basic and applied studies necessary to expand the knowledge and use of enzymes in soil management are pointed out. Soil enzymes play a key role in numerous soil processes, thereby making them useful indicators of productive capacity and soil quality. Research on enzymes in soil has developed significantly in the last two decades, which has made it possible for farmers to analyze and interpret enzyme activity in the soil in the laboratory.

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Temperature sensitivity of mineral-enzyme interactions on the hydrolysis of cellobiose and indican by β-glucosidase

Cited by 24 publications

References 49 publications

The Important Role of Enzyme Adsorbing Capacity of Soil Minerals in Regulating β‐Glucosidase Activity

The Important Role of Enzyme Adsorbing Capacity of Soil Minerals in Regulating β‐Glucosidase Activity

Low-Cost Cellulase-Hemicellulase Mixture Secreted by Trichoderma harzianum EM0925 with Complete Saccharification Efficacy of Lignocellulose

Contribution of enzymes to soil quality and the evolution of research in Brazil

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