Cold acclimation of etiolated cucumber seedlings, consisting of cooling at 12 degrees C for 48 h followed by a warming period at 25 degrees C, led to tolerance to subsequent chilling at 2 degrees C. Tolerance, as evidenced by freedom from chilling injury and continued growth, developed during the warming period in a time-course manner for 12 h but decreased with prolonged warming. A similar increase and subsequent decrease was also observed in the content of palmitic, linoleic and linolenic acids in total lipid fraction from cucumber hypocotyl tissue. During the warming period supra-ambient oxygen stimulated, whereas subambient oxygen inhibited, the increase in fatty acid content as well as development of chilling tolerance. A strong correlation between oxygen-mediated changes in fatty acid content and associated development of cold tolerance suggests that both these processes are interrelated. Cold acclimation, but not cold stress, led to an increase followed by a decrease in CO2 evolution suggesting that a respiratory upsurge is yet another feature of cold acclimation in cucumbers.