Temperature variations within and between incubators—a prospective, observational study

Walker, Meredith W.; Butler, Julia M.; Higdon, H. Lee; Boone, William R.

doi:10.1007/s10815-013-0104-0

Cited by 17 publications

(11 citation statements)

References 6 publications

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“…A preliminary report indicated slight temperature variations when culture dishes were placed in various locations within a large-box water-jacketed incubator, with measurements of 36.97°C, 37.17°C and 37.23°C (Stoddart et al, 2003). Similar findings were also recently reported between two large-box incubator, where minor (0.07-0.17°C), but significant temperature differences were identified between shelves and between incubators (Walker et al, 2013). Whether such minor fluctuations have an impact is unknown, but independent temperature measurement between shelves on box-type units, between individual culture chambers or across warmed surfaces of various benchtop/topload unit configurations can provide insight into temperature accuracy, as well as possible variation that could impact gamete and embryo development and function.…”

Section: Temperaturesupporting

confidence: 76%

Decisions for the IVF laboratory: comparative analysis of embryo culture incubators

Swain

2014

Reproductive BioMedicine Online

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Incubators in the IVF laboratory play a pivotal role in providing a stable and appropriate culture environment required for optimizing embryo development and clinical outcomes. With technological advances, several types of incubators are now available and careful consideration is required for selection. Examination of variables, such as recovery/stabilization of temperature, gas atmosphere and humidity, as well as understanding various approaches utilized by each device to regulate these variables, is critical. Additionally, a comprehensive examination of clinical studies that compare various incubators may provide insight into their efficacy. Other factors, both technical and practical, must also be considered when selecting an incubator. Importantly, proper management, including patient volume and workflow, is paramount in optimizing function of any incubator, regardless of the technology incorporated. This review highlights incubator function and reviews key environmental variables controlled and the technology utilized in various units. Additionally, existing comparative studies focused on incubator recovery and clinical outcomes are critically analysed. Finally, strategies employed for incubator management, as well as future potential incubator improvements are discussed. While existing reports indicate that smaller benchtop/topload incubators provide faster recovery of environmental variables, there is no clear advantage of any particular incubator based on clinical outcomes.

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Section: Temperaturesupporting

confidence: 76%

Decisions for the IVF laboratory: comparative analysis of embryo culture incubators

Swain

2014

Reproductive BioMedicine Online

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“…Temperature sensitivity of preimplantation embryos is less well studied. While one study on incubator performance reported an increased pregnancy rate from incubators with a temperature of 36.96 ± 0.13°C compared with those with a temperature of 37.03 ± 0.13°C (Higdon et al, 2008), another that reported slight temperature gradients of up to 0.3°C between shelves in culture incubators found no significant impact on clinical outcomes (Walker et al, 2013). Of importance, accuracy of these minor temperature variances is less than the uncertainty of measurement of most thermometers.…”

Section: Temperature In the Ivf Laboratorymentioning

confidence: 99%

‘There is only one thing that is truly important in an IVF laboratory: everything’ Cairo Consensus Guidelines on IVF Culture Conditions

Group¹

2020

Reproductive BioMedicine Online

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Cairo 2018 Consensus Group* KEY MESSAGE This report presents outcomes from an international expert meeting to establish consensus guidelines on IVF culture. Topics reviewed were: embryo culture; temperature; humidity; gas control, pH; workstations; incubators; micromanipulation; handling and assessment; stasis, composition, supplementation, type of culture and storage; equipment and monitoring. More than 50 consensus guideline points were established.

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“…vicina (22), P. casei (23) and L. sericata (19), are more likely to provide problems in PMI estimation using ADD following refrigeration as their continued development may skew the PMI estimation if refrigerator temperatures fluctuate too greatly. Walker et al (24), recommends that the temperatures within refrigerators must be evaluated and if significant variations are present appropriate measures must be taken in the analysis of insects taken from human remains. This is an area for concern though, as it requires death-scene technicians and/or mortuary staff to ensure they are recording these temperatures and that the temperatures are not merely assumed to be that of the set temperature of the refrigerator.…”

Section: Discussionmentioning

confidence: 99%

Modeling Temperature Variations Using Monte Carlo Simulation: Implications for Estimation of the Postmortem Interval Based on Insect Development Times*†

Hill

Gilbert

Coetzee

2020

Journal of Forensic Sciences

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The association between insect development and temperature is well established. Thermal summation using accumulated degree-day measures is commonly used. However, the time at which evidence is collected is important in these estimates. The aim of this study was to provide a simulated model of the effect of temperatures on six dipteran species commonly associated with cadavers, from the death scene to the refrigerator, and finally at the time of autopsy. Temperatures measurements were sampled over a 16-month period from the external environment (external to the mortuary), within the mortuary refrigerator, and within the mortuary autopsy suite. Monte Carlo simulation using accumulated degree-days (ADD) was used to estimate the variations based on the mean and standard deviation of the temperature measurements. It was found that there was a negative correlation between the base temperature of the fly species (lowest temperature at which the flies will survive) and developmental likelihood. Species with high base temperatures (Chrysomya albiceps, Chrysomya chloropyga, and Musca domestica) were less likely to continue development in refrigerators than species with lower base temperatures (Lucilia sericata and Piophila casei). The findings of this study highlight the importance of recording temperature measurements and the period of refrigeration on PMI estimation especially when continued development occurs in spite of a period of cooling of the insect evidence.

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Temperature variations within and between incubators—a prospective, observational study

Cited by 17 publications

References 6 publications

Decisions for the IVF laboratory: comparative analysis of embryo culture incubators

Decisions for the IVF laboratory: comparative analysis of embryo culture incubators

‘There is only one thing that is truly important in an IVF laboratory: everything’ Cairo Consensus Guidelines on IVF Culture Conditions

Modeling Temperature Variations Using Monte Carlo Simulation: Implications for Estimation of the Postmortem Interval Based on Insect Development Times*†

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