Template‐directed nucleation and growth of inorganic materials

Heywood, Brigid R.; Mann, Stephen

doi:10.1002/adma.19940060103

Cited by 318 publications

(226 citation statements)

References 55 publications

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“…This does not mean that prisms grow "passively." Instead, prisms are likely to emerge from a complex interplay of electrostatic, stereochemical, and geometrical interactions (107).…”

Section: Discussionmentioning

confidence: 99%

Caspartin and Calprismin, Two Proteins of the Shell Calcitic Prisms of the Mediterranean Fan Mussel Pinna nobilis

Marin

Amons

Guichard

et al. 2005

Journal of Biological Chemistry

133

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We used the combination of preparative electrophoresis and immunological detection to isolate two new proteins from the shell calcitic prisms of Pinna nobilis, the Mediterranean fan mussel. The amino acid composition of these proteins was determined. Both proteins are soluble, intracrystalline, and acidic. The 38-kDa protein is glycosylated; the 17-kDa one is not. Ala, Asx, Thr, and Pro represent the dominant residues of the 38-kDa protein, named calprismin. An N-terminal sequence was obtained from calprismin. This sequence, which comprises a pattern of 4 cysteine residues, is not related to any known protein. The second protein, named caspartin, exhibits an unusual amino acid composition, since Asx constitutes by far the main amino acid residue. Preliminary sequencing surprisingly suggests that the first 75 N-terminal residues are all Asp. Caspartin self-aggregates spontaneously into multimers. In vitro tests show that it inhibits the precipitation of calcium carbonate. Furthermore, it strongly interferes with the growth of calcite crystals. A polyclonal antiserum raised against caspartin was used to localize this protein in the shell by immunogold. The immunolocalization demonstrates that caspartin is distributed within the prisms and makes a continuous film at the interface between the prisms and the surrounding insoluble sheets. Our finding emphasizes the prominent role of aspartic acid-rich proteins for the building of calcitic prisms among molluscs.The secretion of a shell by molluscs is a striking example of a selfassembling process performed outside living tissues. When a mollusc builds its shell, the calcifying epithelium of its mantle extrudes mineral ions, mainly calcium and bicarbonate. In addition, it secretes an extracellular matrix composed of proteins, glycoproteins, proteoglycans, and polysaccharides (1). This mixture is released into the extrapallial space, a microvolume delimited by the epithelium, the growing shell, and the leathery periostracum (2). In this supersaturated space, the released macromolecules interact with bicarbonate and calcium to form biocrystallites that self-aggregate in an orderly manner. The end product is a densely packed organomineral assembly, the shell, in which the mineral phase represents more than 95% by weight (3). As shell proteins exert a control on the biomineralization process, they can be used in the synthesis of biomimetic materials (4 -6). However, the resolution of the primary structure of molluscan shell proteins has been seriously impaired for decades by their polydispersity, their polyanionic properties, and finally their post-translational modifications (3, 7).Despite these obstacles, investigations on molluscan shell proteins have made significant advances in the last 6 years, with the characterization of 16 proteins and, for most of them, the identification of the corresponding transcript (8, 9). Among the different shell textures that molluscs use to produce their shell, mother-of-pearl has received the greatest deal of attention. Its intrinsic bea...

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“…This does not mean that prisms grow "passively." Instead, prisms are likely to emerge from a complex interplay of electrostatic, stereochemical, and geometrical interactions (107).…”

Section: Discussionmentioning

confidence: 99%

Caspartin and Calprismin, Two Proteins of the Shell Calcitic Prisms of the Mediterranean Fan Mussel Pinna nobilis

Marin

Amons

Guichard

et al. 2005

Journal of Biological Chemistry

133

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“…Langmuir monolayers, [3] protein-coated substrates, [4] polymer dispersions, [5] micellar media, [6] and self-assembled monolayers (SAMs) [7±9] have been used as templates or substrates for the crystallization of minerals such as calcium carbonate. We demonstrate here that standard SAM chemistry can be extended to produce controlled nanoscopic surfaces for the heterogeneous nucleation of inorganic minerals from aqueous solution.…”

Section: Dedicated To Professor Bernt Krebs On the Occasion Of His 60mentioning

confidence: 99%

Crystallization of Calcite Spherules around Designer Nuclei

Küther

Seshadri

Tremel

1998

Angewandte Chemie International Edition

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“…Calcium carbonate (CaCO 3 ) one of the most common mineral has many industrial uses such as fillers, dentifrices, and has great biological role as structural supports in skeletons [1][2][3][4][5][6][7][8]. Hence the morphological and polymorphic studies of CaCO 3 under various conditions have gained much importance in the past few decades.…”

Section: Introductionmentioning

confidence: 99%

Polymorphic and Morphological Transformations of CaCO<sub>3</sub> under CO<sub>2</sub> Atmosphere and under the Influence of EDTA at 60 °C

Bhuvaneswari¹,

Kumaravel

Subramani³

et al. 2015

ILCPA

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Abstract. Calcium carbonate scale samples were synthesized from calcium chloride solution in the presence of ethylenediamminetetraacetic acid (EDTA) by bubbling CO 2 gas. Samples were prepared with different concentrations of EDTA at 60 o C and characterized using XRD, FTIR and SEM techniques. The details revealed that EDTA stabilizes aragonite more efficiently at higher concentrations of EDTA. It was also observed that increasing the concentration of EDTA has no significant impact on the polymorphic specificity where as influence the morphology and the size of the particles.

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Template‐directed nucleation and growth of inorganic materials

Cited by 318 publications

References 55 publications

Caspartin and Calprismin, Two Proteins of the Shell Calcitic Prisms of the Mediterranean Fan Mussel Pinna nobilis

Caspartin and Calprismin, Two Proteins of the Shell Calcitic Prisms of the Mediterranean Fan Mussel Pinna nobilis

Crystallization of Calcite Spherules around Designer Nuclei

Polymorphic and Morphological Transformations of CaCO<sub>3</sub> under CO<sub>2</sub> Atmosphere and under the Influence of EDTA at 60 °C

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