Template-free enzymatic synthesis of electrically conducting polyaniline using soybean peroxidase

Cruz-Silva, Rodolfo; Romero‐García, Jorge; Angulo-Sánchez, J.L.; Ledezma‐Pérez, Antonio; Arias, Eduardo; Moggio, Ivana; Flores-Loyola, Erika

doi:10.1016/j.eurpolymj.2004.11.012

Cited by 82 publications

(42 citation statements)

References 34 publications

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“…Enzymatic synthesis also allows the control of regioregularity and stereochemistry of the resulting polymer [22]. Enzymatic polymerization has been proven by polypyrrole and polyaniline synthesis using horseradish peroxidase [23e26], laccase [27,28], soybean peroxidase [29], royal palm tree peroxidase [30] and glucose oxidase [6,31], enzymes, which mostly catalyse the generation of respective monomer free radicals. These free radicals undergo coupling to produce dimmers.…”

Section: Introductionmentioning

confidence: 99%

Enzymatic polymerization of polythiophene by immobilized glucose oxidase

Krikstolaityte

Kuliesius

Ramanavičienė

et al. 2014

Polymer

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Section: Introductionmentioning

confidence: 99%

Enzymatic polymerization of polythiophene by immobilized glucose oxidase

Krikstolaityte

Kuliesius

Ramanavičienė

et al. 2014

Polymer

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“…SBP has better stability and activity than HRP in acidic pHs, and therefore was used here for higher yield. [28][29][30] The dithiol bond in DTSSP was used to form SAMs on the Au electrode. The succinimide group reacts with the primary amine of SBP to form covalent bonds.…”

Section: Resultsmentioning

confidence: 99%

Application of Polyaniline to an Enzyme-Amplified Electrochemical Immunosensor as an Electroactive Report Molecule

Kwon¹,

Seo²,

Yang³

et al. 2010

Bulletin of the Korean Chemical Society

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Conducting polymers (CPs) are widely used as matrixes for the entrapment of enzymes in analytical chemistry and biosensing devices. However, enzyme-catalyzed polymerization of CPs is rarely used for immunosensing due to the difficulties involved in the quantitative analysis of colloidal CPs in solution phase. In this study, an enzyme-amplified electrocatalytic immunosensor employing a CP as a redox marker has been developed. A polyanionic polymer matrix, α-amino-ω-thiol terminated poly(acrylic acid), was employed for precipitation of CP. The acrylic acid group acts as a polyanionic template. The thiol terminus of the polymer was used to produce self-assembled monolayers (SAMs) on Au electrodes and the amine terminus was employed for immobilization of biomolecules. In an enzymeamplified sandwich type immunosensor, the polyaniline (PANI) produced enzymatically is attracted by the electrostatic force of the matrix polymer. The precipitated PANI was characterized by electrochemical methods.

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“…The approach does not require strong acids or purification steps in the synthesis process. Enzymes can offer environmentally benign reaction conditions [7], a high yield of polymerization, and a superior level of control in regioregularity and stereochemistry, consequently resulting in soluble and processable conducting polymers [8].…”

Section: Introductionmentioning

confidence: 99%

Oxireductases in the Enzymatic Synthesis of Water-Soluble Conducting Polymers

Ochoteco

Mecerreyes

2010

Advances in Polymer Science

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This chapter reviews recent advances in the field of biocatalytic synthesis of water-soluble conducting polymers. Biocatalysis is proposed as a versatile tool for synthesis of conducting polymers. First, the enzymatic synthesis of conducting polymers and its mechanism is discussed as well as the use of different type of enzymes. Next, we describe the use of a new bifunctional template (sodium dodecyl diphenyloxide disulfonate) in the synthesis of polyaniline as a strategy to improve the water solubility and electrical conductivity in the obtained polymer. The recent development of enzyme-catalyzed polymerization of 3,4-ethylenedioxythiophene (EDOT) in the presence of polystyrenesulfonate is discussed. This method results in PEDOT materials that show an electrical conductivity of 2 × 10 −3 S cm −1 and posses excellent film formation ability, as confirmed by atomic force microscopy images. Finally, a simple method for immobilizing horseradish peroxidases in the biocatalytic synthesis of water-soluble conducting polymers is presented. This method is based on a biphasic catalytic system in which the enzyme is encapsulated inside the ionic liquid 1-butyl-3-methylimidazolium hexafluorophosphate, while other components remain in the aqueous phase. The enzyme is easily recovered after reaction and can be reused several times.

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Template-free enzymatic synthesis of electrically conducting polyaniline using soybean peroxidase

Cited by 82 publications

References 34 publications

Enzymatic polymerization of polythiophene by immobilized glucose oxidase

Enzymatic polymerization of polythiophene by immobilized glucose oxidase

Application of Polyaniline to an Enzyme-Amplified Electrochemical Immunosensor as an Electroactive Report Molecule

Oxireductases in the Enzymatic Synthesis of Water-Soluble Conducting Polymers

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