Tempol protects against intravitreous indocyanine green-induced retinal damage in rats

Thaler, Sebastian; Voykov, Bogomil; Willmann, Gabriel; Fiedorowicz, Michał; Ręjdak, Robert; Gekeler, Florian; May, Christian Albrecht; Schatz, Andreas; Schuettauf, Frank

doi:10.1007/s00417-012-2000-1

Cited by 6 publications

(6 citation statements)

References 44 publications

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“…In contrast, a previous study with ICG 0.5% in the same model showed a much more pronounced b-wave reduction that remained significant even 14 days after ICG application. 17 This accords with the results of other ERG studies 3 in which b-wave reduction was significant even after 2 months of dye exposure, and the retinal structure was altered. Other studies found significant b-wave reductions for ICG 0.5%; these persisted for o4 weeks but were accompanied by anatomical changes.…”

Section: In Vivo Testing Of a New Cyanine Dye S Thaler Et Alsupporting

confidence: 90%

“…Electrodes were placed as described previously. 17 Ten minutes before starting ERG measurements, the pupils were fully dilated with tropicamide and the dim red light was turned off.…”

Section: Electroretinographymentioning

confidence: 99%

“…13 A well-established experimental setting with a rat model was used in the present in vivo study. 14,17 We chose to test DSS at a concentration of 0.5%. This had no adverse effects in different cell lines for up to 120 s of exposition time and was the highest concentration used in vitro for optimal staining of the ILM.…”

Section: In Vivo Testing Of a New Cyanine Dye S Thaler Et Almentioning

confidence: 99%

“…Importantly, this concentration also afforded optimum comparability with previously performed toxicity studies of ICG in the same animal model. 17,26 Assessment of RGC numbers after retrograde labelling with a fluorescent marker is a sensitive tool for evaluating the loss of functional RGC in vivo. [27][28][29][30] In addition to analysis of retinal layer thicknesses, it allows convenient and very reliable direct evaluation of RGC damage.…”

Section: In Vivo Testing Of a New Cyanine Dye S Thaler Et Almentioning

confidence: 99%

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In vivo biocompatibility of a new cyanine dye for ILM peeling

Thaler

Haritoglou

Schuettauf

et al. 2014

Eye

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Section: In Vivo Testing Of a New Cyanine Dye S Thaler Et Alsupporting

confidence: 90%

“…Electrodes were placed as described previously. 17 Ten minutes before starting ERG measurements, the pupils were fully dilated with tropicamide and the dim red light was turned off.…”

Section: Electroretinographymentioning

confidence: 99%

Section: In Vivo Testing Of a New Cyanine Dye S Thaler Et Almentioning

confidence: 99%

Section: In Vivo Testing Of a New Cyanine Dye S Thaler Et Almentioning

confidence: 99%

See 2 more Smart Citations

In vivo biocompatibility of a new cyanine dye for ILM peeling

Thaler

Haritoglou

Schuettauf

et al. 2014

Eye

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“…The present study reinforces the toxic profile of ICG showing a reduction of RPE cell viability in all tested concentrations and time exposure. More recently some papers have been dedicated to reduce the ICG-related toxicity with free radical scarvengers (tampol), less concentration, short time exposure, or iodine-free solution such as IfCG [35] [36, 37]. …”

Section: Discussionmentioning

confidence: 99%

Effect of Vital Dyes on Retinal Pigmented Epithelial Cell Viability and Apoptosis: Implications for Chromovitrectomy

et al. 2013

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Purpose: To investigate the in vitro effect of vital dyes on toxicity and apoptosis in a human retinal pigment epithelial cell line. Methods: ARPE-19 cells were exposed to brilliant blue (BBG), Evans Blue (EB), bromophenol blue (BroB), indocyanine green (ICG), infracyanine green (IfCG), light green (LG), fast green (FG), indigo carmine (IC) and Congo red (CR). Balanced salt solution was used as the control. Five different concentrations and 2 exposure times were tested. Cell viability was determined by the MTS (1-solution methyl thiazolyl tetrazolium) assay and apoptosis by Bax expression on Western blot. Results: All dyes significantly reduced cell viability after 3 min of exposure at all concentrations (p < 0.01), except for BBG that was safe at concentrations up to 0.25 mg/ml and CR up to 0.05 mg/ml, while LG was safe at all concentrations. Toxicity was higher after 30 min of exposure. Expression of Bax was upregulated after all dye exposures, except BBG; ICG had the highest Bax expression (p < 0.01). Conclusions: Overall the safest dye was BBG followed by LG, IfCG, FG, CR, IC, BroB, EB and ICG. ICG was toxic at all concentrations and exposure times tested. Moreover, BBG was the only dye that did not induce apoptosis in ARPE-19 cells.

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Neuroprotektive Ansätze

2013

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After introduction of vitreoretinal surgery more than 40 years ago, further development of the procedure involved a continuous reduction of potential toxic effects by irrigating solutions, endoillumination or mechanical manipulation. Recently, additional efforts were made to prevent neurodegeneration via pharmacological intervention. Taurine as additive for irrigating solutions can be considered as an example for neuroprotectants in vitreoretinal surgery. Approval of neuroprotective agents demands an increased effort for preclinical and clinical evaluation. To date, only few neuroprotective substances are used in clinical routine in the context of vitreoretinal surgery, however, experimental data suggest a high potential of various neuroprotective agents. The following article gives an overview of current neuroprotective approaches feasible for vitreoretinal surgery and a critical analysis of their clinical relevance.

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Tempol protects against intravitreous indocyanine green-induced retinal damage in rats

Abstract: Tempol significantly attenuates ICG-induced toxicity in rat retinas and may therefore be considered for further evaluation as accompanying treatment in ICG-assisted chromovitrectomy.

Cited by 6 publications

References 44 publications

In vivo biocompatibility of a new cyanine dye for ILM peeling

In vivo biocompatibility of a new cyanine dye for ILM peeling

Effect of Vital Dyes on Retinal Pigmented Epithelial Cell Viability and Apoptosis: Implications for Chromovitrectomy

Neuroprotektive Ansätze

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