Temporal acclimation of Microchloropsis gaditana CCMP526 in response to hypersalinity

A Data-Independent-Acquisition-based proteomic approach towards understanding the acclimation strategy ofMicrochloropsis gaditanaCCMP526 in hypersaline conditions

“…However, a varied response to different saline conditions was evident across the time points. Salinity-induced ROS accumulation in cells at 55 PSU was observed in our previous study (Karthikaichamy et al, 2018) and correspondingly, the GO-terms for response to stress (GO:0033554) and cellular oxidant detoxification (GO:0098869) were enriched at 6 and 24 h respectively.…”

“…In contrast, GO-terms for energy-intensive translation (GO:0006412) was enriched at 1 and 6 h after transfer of cells to 100 PSU, suggesting a protein aided metabolic shift during the later phases (72 h) of acclimation through carbohydrate metabolism (GO:0005975) and homeostatic processes (GO:0042592). The enrichment of the carbohydrate metabolism (GO:0005975) term during the later-phase of growth suggests carbon shunting towards lipid accumulation, which is evident from our physiological (Karthikaichamy et al, 2018) and transcriptomic studies on M. gaditana (un-published).…”

“…DIA based proteomic analysis was employed to investigate the temporal acclimatization strategy of M. gadit ana at different salinity levels (55 and 100 PSU) compared to controls at 38 PSU, and at various time points (0, 1, 6, 24 and 72 h). These time points represent the critical stages of change in cellular state in regard to alterations in photosynthetic rate, carbohydrate, and lipid accumulation (Karthikaichamy et al, 2018). Changes in protein level are represented as average log 2 (fold change) compared with the control (38 PSU) sample at corresponding time points (figure 1A).…”

“…Following our previous study on M. gaditana during the transfer to high-salinity conditions (Karthikaichamy et al, 2018), GO-terms for carbohydrate metabolism were enriched after 72 h in 100 PSU. Relatively fewer GO-terms were enriched at 6 and 24 h after transfer, which is a reflection of the number of differentially expressed proteins in cells growing at 55 and 100 PSU.…”

“…Similarly, we observed salinity induced dynamics in the transcriptome of M. gaditana (un-published). In order to understand the fate of cellular metabolism at high saline conditions, and to connect a line between physiology (Karthikaichamy et al, 2018) and transcriptome (un-published) of salinity induced M. gaditana , the differentially expressed proteins were analyzed for their biological significance in the following sections.…”