Temporal analysis of the microbiota involved in the anaerobic degradation of sugarcane vinasse in a full-scale methanogenic UASB reactor

Callejas, Cecilia; López, Iván; Bovio-Winkler, Patricia; Etchebehere, Claudia; Borzacconi, Liliana

doi:10.1007/s13399-021-01281-8

Cited by 14 publications

(10 citation statements)

References 41 publications

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“…The adaptation of microbiota to the feed was also confirmed by Callejas et al [99] which evaluated the temporal evolution of the microbial community in a full-scale UASB reactor treating sugarcane vinasse. They noted the progressive reduction in the microbial diversity since the inoculum and the spontaneous selection of a less diverse microbiota to treat organic substances producing methane.…”

Section: Microbiota and Microbiome In Uasb Reactorsmentioning

confidence: 59%

“…They noted the progressive reduction in the microbial diversity since the inoculum and the spontaneous selection of a less diverse microbiota to treat organic substances producing methane. In the treatment of vinasse, they also suggested that Firmicutes played a key role due to their abundance [ 99 ].…”

Section: Resultsmentioning

confidence: 99%

“…The study of the microbiota and microbiome of the granular biomass of UASB reactors has been the subject of intense research. Studies have confirmed that the microbiota is able to change its structure to better adapt to external stimuli such as conditions of temperature, feed, and pollutants (e.g., [ 95 , 96 , 97 , 98 , 99 ]). These works could be the starting point for future studies aimed at better exploiting this biomass “adaptation” capacity by optimizing the operation of UASB reactors and increasing their performance.…”

Section: Discussion About Literature Results Gaps and Tips For Future Researchmentioning

confidence: 99%

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Applications of Up-Flow Anaerobic Sludge Blanket (UASB) and Characteristics of Its Microbial Community: A Review of Bibliometric Trend and Recent Findings

Collivignarelli

Abbà

Caccamo

et al. 2021

IJERPH

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The interest in research on up-flow anaerobic sludge blanket (UASB) reactors is growing. The meta-analysis of bibliometric data highlighted the growing interest in four diverse topics: (i) energy recovery production; (ii) combination with other treatments; (iii) the study of processes for the removal of specific pollutants and, (iv) characterization of microbial community and granular sludge composition. In particular, the papers published in the first 6 months of 2021 on this process were selected and critically reviewed to highlight and discuss the results, the gaps in the literature and possible ideas for future research. Although the state of research on UASB is to be considered advanced, there are still several points that will be developed in future research such as the consolidation of the results obtained on a semi-industrial or real scale, the use of real matrices instead of synthetic ones and a more in-depth study of the effect of substances such as antibiotics on the microbiota and microbiome of UASB granular biomass. To date, few and conflicting data about the environmental footprint of UASB are available and therefore other studies on this topic are strongly suggested.

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Section: Microbiota and Microbiome In Uasb Reactorsmentioning

confidence: 59%

Section: Resultsmentioning

confidence: 99%

Section: Discussion About Literature Results Gaps and Tips For Future Researchmentioning

confidence: 99%

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Applications of Up-Flow Anaerobic Sludge Blanket (UASB) and Characteristics of Its Microbial Community: A Review of Bibliometric Trend and Recent Findings

Collivignarelli

Abbà

Caccamo

et al. 2021

IJERPH

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“…A distribuição taxonômica da comunidade microbiana extraída do pneu triturado do RALF-MT em 10 kg DQO m -3 d - ), maior tolerância ao íon amônio (<7,000 mg L -1 ) e maior tolerância a elevadas concentrações de acetato (<15,000 mg L -1 ) do que Methanotrhix (0,2 d -1 , <3,000 mg L -1 e <3,000 mg L -1 , respectivamente) (DE VRIEZE et al, 2012) Em relação ao domínio Bactéria, os microrganismos pertencentes aos filos Bacteroidetes, Firmicutes e Proteobacteria foram os mais abundantes (Figura 24). Estes filos estão dentre os mais abundantes em reatores de digestão anaeróbia independentemente do substrato utilizado (CALLEJAS et al, 2021). Contudo, elevadas temperaturas, como 55 °C, são associadas a redução da abundância relativa de Bacteroidetes e Proteobacteria e aumento da abundância relativa de Firmicutes (HUPFAUF et al, 2018;LIU et al, 2018).…”

Section: Composição Microbiana No Ralf-mt Em 100 Kg Dqo M -3 D -1 E 5...unclassified

“…Contudo, elevadas temperaturas, como 55 °C, são associadas a redução da abundância relativa de Bacteroidetes e Proteobacteria e aumento da abundância relativa de Firmicutes (HUPFAUF et al, 2018;LIU et al, 2018). Isto porque Firmicutes são capazes de degradar uma ampla variedade de substratos em condições adversas de temperatura e salinidade (CALLEJAS et al, 2021). Em contrapartida, Bacteroidetes são atribuídas a maior eficiência na degradação de fenóis, aminoácidos e aminas do que Firmicutes (SCHIEVANO et al, 2012).…”

Section: Composição Microbiana No Ralf-mt Em 100 Kg Dqo M -3 D -1 E 5...unclassified

Co-digestão de vinhaça e glicerina em reatores anaeróbios de leito fluidificado

Menezes¹

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Microbial community evolution in a lab-scale reactor operated to obtain biomass for biochemical methane potential assays

de la Sovera,

Bovio-Winkler,

Zinola

et al. 2024

Appl Microbiol Biotechnol

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Biochemical methane potential (BMP) test is an important tool to evaluate the methane production biodegradability and toxicity of different wastes or wastewaters. This is a key parameter for assessing design and feasibility issues in the full-scale implementation of anaerobic digestion processes. A standardized and storable inoculum is the key to obtain reproducible results. In Uruguay, a local enterprise dedicated to design and install anaerobic digesters operated a lab-scale bioreactor as a source of biomass for BMP tests, using a protocol previously described. This reactor was controlled and fed with a mixture of varied organic compounds (lipids, cellulolytic wastes, proteins). Biomass was reintroduced into the reactor after BMP assays to maintain a constant volume and biomass concentration. The aim of this work was to evaluate how the microbial community evolved during this operation and the effect of storing biomass in the refrigerator. The composition of the microbial communities was analyzed by 16S rRNA amplicon sequencing using primers for Bacteria and Archaea. The methanogenic activity was determined, and the methanogens were quantified by mcr A qPCR. One sample was stored for a 5-month period in the refrigerator (4 °C); the activity and the microbial community composition were analyzed before and after storage. Results showed that applying the reported methodology, a reliable methanogenic sludge with an acceptable SMA was obtained even though the reactor suffered biomass alterations along the evaluated period. Refrigerating the acclimatized biomass for 5 months did not affect its activity nor its microbial composition according to the 16S rRNA gene sequence analysis, even though changes in the mcr A abundance were observed. Key points • The applied methodology was successful to obtain biomass suitable to perform BMP assays. • The microbial community was resilient to external biomass addition. • Biomass storage at 4 °C for 5 months did not alter the methanogenic activity. Graphical Abstract Supplementary Information The online version contains supplementary material available at 10.1007/s00253-024-13305-0.

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Temporal analysis of the microbiota involved in the anaerobic degradation of sugarcane vinasse in a full-scale methanogenic UASB reactor

Cited by 14 publications

References 41 publications

Applications of Up-Flow Anaerobic Sludge Blanket (UASB) and Characteristics of Its Microbial Community: A Review of Bibliometric Trend and Recent Findings

Applications of Up-Flow Anaerobic Sludge Blanket (UASB) and Characteristics of Its Microbial Community: A Review of Bibliometric Trend and Recent Findings

Co-digestão de vinhaça e glicerina em reatores anaeróbios de leito fluidificado

Microbial community evolution in a lab-scale reactor operated to obtain biomass for biochemical methane potential assays

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