Temporal and spatial distribution of transcripts from the <i>Deformed</i>
 gene of <i>Drosophila</i>

Chadwick, Robin; McGinnis, William

doi:10.1002/j.1460-2075.1987.tb04820.x

Cited by 92 publications

(34 citation statements)

References 40 publications

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“…However, the pattern of pb protein expression in the central nervous system differs strikingly from the patterns of transcript and/or protein expression in the central nervous system observed for the other ANT-C homeotic genes (Carroll et al 1986;Wirz et al 1986;Mahaffey and Kaufman 1987;Riley et al 1987;Chadwick and McGinnis 1987;Martinez-Arias et al 1987;R. Diederich, unpubl.).…”

Section: Cold Spring Harbor Laboratory Press On March 25 2019 -Publimentioning

confidence: 70%

The proboscipedia locus of the Antennapedia complex: a molecular and genetic analysis.

Pultz¹,

Diederich²,

Cribbs³

et al. 1988

Genes Dev.

117

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The homeotic proboscipedia (pb) locus of the Antennapedia Complex (ANT-C) directs the differentiation of adult labial and maxillary structures. Loss-of-function pb alleles show a transformation of adult mouth parts to legs and affect maxillary palp morphology. We have identified the pb transcription unit by inducing and analyzing a series of pb null chromosomal breakpoints. In addition, we describe a variegating pb phenotype associated with two rearrangement breakpoints. Having identified the pb locus, we describe the expression of its RNA and protein products. Unlike the other homeotic genes of the ANT-C, pb has no obvious role in embryonic development. Nevertheless, pb protein is detected during embryogenesis in nuclei of the labial and maxillary lobes and in part of the mandibular segment. In this respect, pb expression parallels the early segment-specific expression of neighboring, embryonically essential homeotic genes. Accumulation of pb protein is also detected in mesodermal cells and in a unique subset of nuclei throughout the central nervous system. We also describe a transcription unit very close to pfc, which is expressed dorsally during embryogenesis in a pattern resembling that of the nearby zygotic lethal zerkniillt (zen) locus. This transcription unit has been shown to contain a homeo box and has been designated z2. Surprisingly, we find that individuals deleted for both the pb and z2 transcription units survive to adulthood and produce normal larval cuticular structures. Thus, pb and z2 show similarities to neighboring ANT-C genes in their expression patterns, yet these similarities are not manifested in comparable loss-of-function embryonic phenotypes.

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Section: Cold Spring Harbor Laboratory Press On March 25 2019 -Publimentioning

confidence: 70%

The proboscipedia locus of the Antennapedia complex: a molecular and genetic analysis.

Pultz¹,

Diederich²,

Cribbs³

et al. 1988

Genes Dev.

117

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“…Mis-expressed Dfd does not repress optix in the head or in the gnathal segments, the normal domain of Dfd expression ( Fig. 5F) (Chadwick and McGinnis, 1987); however, the gnathal patches of optix are enlarged (compare Fig. 5F, Fig.…”

Section: Research Articlementioning

confidence: 96%

“…Dfd is a divergent C type Hox gene (Schubert et al, 1993), expressed in the maxillary and mandibular segments in wild-type embryos (Chadwick and McGinnis, 1987). Loss of Dfd induces ectopic optix expression in the ventral part of the maxillary segment (arrow, Fig.…”

Section: The Role Of Optix In Head Developmentmentioning

confidence: 99%

Common functions of central and posterior Hox genes for the repression of head in the trunk ofDrosophila

2008

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Hox genes are localised in complexes, encode conserved homeodomain transcription factors and have mostly been studied for their specialised functions: the formation of distinct structures along the anteroposterior axis. They probably derived via duplication followed by divergence, from a unique gene, suggesting that Hox genes may have retained a common function. The comparison of their homeodomain sequences groups Hox proteins into Anterior, Central and Posterior classes, reflecting their expression patterns in the head, trunk and tail, respectively. However, functional data supporting this classification are rare. Here, we re-examine a common activity of Hox genes in Drosophila: the repression of head in the trunk. First, we show that central and posterior Hox genes prevent the expression of the head specific gene optix in the trunk, providing a functional basis for the classification. Loss-offunction mutations of optix affect embryonic head development, whereas ectopic Optix expression strongly perturbs trunk development. Second, we demonstrate that the non-Hox genes teashirt, extradenticle and homothorax are required for the repression of optix and that Wingless signalling and Engrailed contribute to this repression. We propose that an evolutionary early function of Hox genes was to modify primitive head morphology with novel functions specialising the trunk appearing later on.

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“…Interestingly, the J3-galactosidase expression pattern in transgenic mouse embryos delineated by the Hox-l.3 enhancer spans the border between the lower cervical and anterior thoracic CNS regions. Dfd, the 3' neighbor to Scr in the ANT-C complex, is expressed in the posterior head, just anterior to the region expressing Scr (Martinez-Arias et al 1987;Chadwick and McGinnis 1987). Similarly, the Hox-5.1 / lacZ expression pattern is found entirely within the upper cervical CNS region, just anterior to the region expressing Hox-l.3/lacZ.…”

Section: Region-specific Enhancers In the Developmental Regulation Ofmentioning

confidence: 99%

Region-specific enhancers near two mammalian homeo box genes define adjacent rostrocaudal domains in the central nervous system.

Tuggle¹,

Zákány²,

Cianetti³

et al. 1990

Genes Dev.

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To gain insight to the mechanisms underlying region-specific gene expression in mammalian development, we investigated the regulatory DNA associated with the proximal promoter of two homeo box genes, murine Hox-1.3 and human Hox-5.1. Using lacZ gene fusions in transgenic mice, we identified regulatory elements in the 5'-flanking sequences of the Hox-l.3 and the Hox-5.1 genes that specifically direct [~-galactosidase expression to the brachial and the upper cervical regions (respectively) of the central nervous system (CNS). These two elements act at the transcriptional level, are active in either orientation, and confer region-specific expression to unrelated promoters, satisfying the criteria for enhancer elements. The two spatial domains defined by these enhancers are directly adjoining, extend along the rostrocaudal axis for the same span of 6-7 metameres, and represent specific subsets of the overall CNS regions expressing all endogenous Hox-l.3 or Hox-5.1 transcripts. The adjacent domains in the developing murine CNS that express Hox-l.3 and Hox-5.1 gene fusions are strikingly reminiscent of the adjacent stripes of expression in Drosophila embryos seen with Sex combs reduced and Deformed, the two Drosophila homeotic genes most homologous to Hox-l.3 and Hox-5.1, respectively. These findings represent the first demonstration of region-specific mammalian enhancers and raise the possibility that the mammalian CNS may be subdivided into a series of rostrocaudal domains on the basis of the activity of enhancers near homeo box genes.

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Temporal and spatial distribution of transcripts from the Deformed gene of Drosophila

Cited by 92 publications

References 40 publications

The proboscipedia locus of the Antennapedia complex: a molecular and genetic analysis.

The proboscipedia locus of the Antennapedia complex: a molecular and genetic analysis.

Common functions of central and posterior Hox genes for the repression of head in the trunk ofDrosophila

Region-specific enhancers near two mammalian homeo box genes define adjacent rostrocaudal domains in the central nervous system.

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