Temporal assessment of root and shoot colonization of elephant grass (Pennisetum purpureum Schum.) host seedlings by Gluconacetobacter diazotrophicus strain LP343

Dos-Santos, Carlos Magno; Nascimento, Wiglison B.A.; Nascimento, Bruna P. do; Schwab, Stefan; Baldani, José Ivo; Vidal, Márcia Soares

doi:10.1016/j.micres.2020.126651

Cited by 5 publications

(1 citation statement)

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“…Surprisingly, our results revealed the improved colonization of IM-FN in soil, which was probably due to the gradual release of the microorganisms, whereas the FN of strains FH-1 and NJ-1 could not persistently colonize the soil. These results were consistent with those previously reported (Dos-Santos et al, 2020). The ability of atrazinedegrading bacteria to persistently colonize the soil was an important factor determining their degradation effect.…”

Section: Colonization Dynamics Of Strain Fh-1 and Nj-1 Of Immobilized...supporting

confidence: 93%

Immobilization of bacterial mixture of Klebsiella variicola FH-1 and Arthrobacter sp. NJ-1 enhances the bioremediation of atrazine-polluted soil environments

Pan

Zhai

et al. 2023

Front. Microbiol.

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In this study, the effects of the immobilized bacterial mixture (IM-FN) of Arthrobacter sp. NJ-1 and Klebsiella variicola strain FH-1 using sodium alginate-CaCl2 on the degradation of atrazine were investigated. The results showed that the optimal ratio of three types of carrier materials (i.e., rice straw powder, rice husk, and wheat bran) was 1:1:1 with the highest adsorption capacity for atrazine (i.e., 3774.47 mg/kg) obtained at 30°C. On day 9, the degradation efficiency of atrazine (50 mg/L) reached 98.23% with cell concentration of 1.6 × 108 cfu/ml at pH 9 and 30°C. The Box–Behnken method was used to further optimize the culture conditions for the degradation of atrazine by the immobilized bacterial mixture. The IM-FN could be reused for 2–3 times with the degradation efficiency of atrazine maintained at 73.0% after being stored for 80 days at 25°C. The population dynamics of IM-FN was explored with the total soil DNA samples specifically analyzed by real-time PCR. In 7 days, the copy numbers of both PydC and estD genes in the IM-FN were significantly higher than those of bacterial suspensions in the soil. Compared with bacterial suspensions, the IM-FN significantly accelerated the degradation of atrazine (20 mg/kg) in soil with the half-life shortened from 19.80 to 7.96 days. The plant heights of two atrazine-sensitive crops (wheat and soybean) were increased by 14.99 and 64.74%, respectively, in the soil restored by immobilized bacterial mixture, indicating that the IM-FN significantly reduced the phytotoxicity of atrazine on the plants. Our study evidently demonstrated that the IM-FN could significantly increase the degradation of atrazine, providing a potentially effective bioremediation technique for the treatment of atrazine-polluted soil environment and providing experimental support for the wide application of immobilized microorganism technology in agriculture.

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Section: Colonization Dynamics Of Strain Fh-1 and Nj-1 Of Immobilized...supporting

confidence: 93%

Immobilization of bacterial mixture of Klebsiella variicola FH-1 and Arthrobacter sp. NJ-1 enhances the bioremediation of atrazine-polluted soil environments

Pan

Zhai

et al. 2023

Front. Microbiol.

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Soil microbiological attributes under the cultivation of Pennisetum purpureum genotypes

Silva,

Santos,

Oliveira

et al. 2024

Rev. Ceres

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Effects of thermotherapy and meristem culture on forage production and nutrition value in elephant grass cultivars

Karasawa¹,

Botega²,

Pinto³

et al. 2023

Agron. Sci. Biotechnol.

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Elephant grass plays important role in dairy cattle production and has received special attention due to its potential in bioenergy and phytoremediation, as well as to its medicinal properties. The aim was investigating the effects of thermotherapy and meristem culture on elephant grass (Cenchrus purpureus (Schumach.) Morrone) forage production and nutritional value. Cultivars “Mineiro”, “Taiwan A-147” and “Pioneiro” were subjected to the methods: thermotherapy plus meristem culture, meristem culture and mature stems (control). The experiment assessed the tiller number (TN), tiller height (TH), number of leaves/tiller (NLT), leaf/stem ratio (LSR), crude protein (CP), acid detergent fiber (ADF), and neutral detergent fiber (NDF), at three cuts performed every 60 days for 180 days. It was observed beneficial influence on the evaluated traits, which indicated how cleaning methods work. Cultivar “Mineiro”, with more than 20 years of use, was more sensitive to the cleaning methods showing than cultivars “Taiwan A-147” (15 years of use) and “Pioneiro” (less than 10 years). It was observed that methods have affected the NT, TH, SDM, LSR, LDM, CP, and NDF. And, that the cultivars genotype effect in a different way the NT, TH, NLT, SDM, LSR, and CP. Overall, Cleaning methods produced an increase in the evaluated parameters over 100% for the cultivars Mineiro and Taiwan when compared with the traditional method and presented low or negative effect for Pioneiro cultivar This evidenced that the traditional propagation method affects forage production and quality over generations in vegetative propagated species. Basic biotechnology techniques such as meristem culture associated or not with thermotherapy can restore the productive potential being recommended for old asexually propagated cultivars with more than fifteen years of cultivation.

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Temporal assessment of root and shoot colonization of elephant grass (Pennisetum purpureum Schum.) host seedlings by Gluconacetobacter diazotrophicus strain LP343

Cited by 5 publications

References 50 publications

Immobilization of bacterial mixture of Klebsiella variicola FH-1 and Arthrobacter sp. NJ-1 enhances the bioremediation of atrazine-polluted soil environments

Immobilization of bacterial mixture of Klebsiella variicola FH-1 and Arthrobacter sp. NJ-1 enhances the bioremediation of atrazine-polluted soil environments

Soil microbiological attributes under the cultivation of Pennisetum purpureum genotypes

Effects of thermotherapy and meristem culture on forage production and nutrition value in elephant grass cultivars

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