Temporal changes in charge content of cultured chondrocytes from bovine cartilaginous tissues

Damme, Markus; Sinnaya, P.; Derry, Kendra L.; Murphy, William H.; Preston, B. N.

doi:10.1016/s0945-053x(97)90022-6

Cited by 4 publications

(3 citation statements)

References 32 publications

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“…Similarly, with each HA‐deposition step, the outermost layer of the cell is rendered negative. Since proteins behave as dipoles,20 variations in the charge magnitudes could be attributed to various matrix proteins, synthesized by the stem cells and deposited in the pericellular matrix,21 or the proteins from the serum in the culture media. The differences in magnitude for the PLL‐deposition steps when compared to the HA steps are a product of the chemical nature of each polyelectrolyte, and the pH at which the layers are assembled.…”

Section: Resultsmentioning

confidence: 99%

Nanoencapsulation of Stem Cells within Polyelectrolyte Multilayer Shells

Veerabadran

Goli

Stewart-Clark

et al. 2007

Macromolecular Bioscience

164

149

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Mouse mesenchymal stem cells have been individually encased by polyelectrolyte layers of poly (L-lysine) and hyaluronic acid using the electrostatic layer-by-layer assembly technique, resulting in a shell consisting of nanolayers of thickness around 6-9 nm. Maintenance of cell morphology and viability were demonstrated for up to one week. Further adjustments to shell permeability and flexibility will facilitate the use of these encapsulated cells in tissue engineering and targeted-delivery applications.

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Section: Resultsmentioning

confidence: 99%

Nanoencapsulation of Stem Cells within Polyelectrolyte Multilayer Shells

Veerabadran

Goli

Stewart-Clark

et al. 2007

Macromolecular Bioscience

164

149

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“…Chondrocytes were isolated from bovine cartilage as previously described 10 , counted and assessed for viability by Trypan blue dye exclusion, and plated in DMEM (Gibco, Grand Island, NY, USA) containing 20% (v/v) heat‐inactivated FCS (Trace Biosciences, Sydney, Australia) and 25 µg/mL of ascorbic acid (Sigma Chemical, St Louis, MO, USA), at high density (∼1−2 × 10 6 cells/cm 2 ) so as to preserve their chondrocytic phenotype. The medium was changed daily and fresh ascorbic acid (25 µg/mL) was added at the time of changes.…”

Section: Methodsmentioning

confidence: 99%

An arthritogenic monoclonal antibody to type II collagen, CII‐C1, impairs cartilage formation by cultured chondrocytes

et al. 2004

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Summary Antibodies to type II collagen (CII) cause articular damage in collagen-induced arthritis (CIA) in mice as judged by passive transfer to naive animals of mAb to CII. We tested the hypothesis that mAb degrade cartilage structure by reacting with functionally important regions of the collagen molecule by examining the effects of an arthritogenic mAb to CII, CII-C1, on cultured bovine chondrocytes at high density, at days 7 and 14. The effects were compared of CII-C1, an isotype-matched control mAb, or medium alone, on chondrocyte proliferation and viability, cell morphology, matrix structure by light and electron microscopy, and matrix synthesis by metabolic labelling with 3 H-proline for collagen or 35 SO 4 for proteoglycans. Chondrocytes in culture remained viable, proliferated, and produced an extracellular matrix in which CII was the major collagen. The addition of CII-C1, but not a control mAb, increased the synthesis of CII and proteoglycan, and caused disorganization of the extracellular matrix and thin collagen fibrils ultrastructurally. Moreover, using a cell-free assay, CII-C1 inhibited the normal selfassembly of collagen fibrils from CII in solution. The finding that the mAb to CII, CII-C1 has striking degradative effects in vitro on cartilage synthesis suggests that antibodies to collagen perpetuate the chronic phase of CIA and that, in mice at least, such antibodies are an important component of pathogenesis.

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“…The presences of negative charges have been reported to be advantageous for the adhesion and spreading of MSCs on ECM substratum [145, 146]. Furthermore, there is evidence that variations in the electrostatic charge content of the peri‐cellular matrix synthesized by chondrocytes have a profound influence on the biomechanical properties of cartilaginous tissue [147]. Surface texture/roughness seems to have differential effects on chondrocyte proliferation, differentiation, and matrix production, depending on the maturational state of the chondrocyte itself [148, 149].…”

Section: Development Of Defined Culture Milieu For Directing the Chonmentioning

confidence: 99%

Directing Stem Cell Differentiation into the Chondrogenic Lineage In Vitro

2004

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A major area in regenerative medicine is the application of stem cells in cartilage tissue engineering and reconstructive surgery. This requires well‐defined and efficient protocols for directing the differentiation of stem cells into the chondrogenic lineage, followed by their selective purification and proliferation in vitro. The development of such protocols would reduce the likelihood of spontaneous differentiation of stem cells into divergent lineages upon transplantation, as well as reduce the risk of teratoma formation in the case of embryonic stem cells. Additionally, such protocols could provide useful in vitro models for studying chondrogenesis and cartilaginous tissue biology. The development of pharmacokinetic and cytotoxicity/genotoxicity screening tests for cartilage‐related biomaterials and drugs could also utilize protocols developed for the chondrogenic differentiation of stem cells. Hence, this review critically examines the various strategies that could be used to direct the differentiation of stem cells into the chondrogenic lineage in vitro.

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Temporal changes in charge content of cultured chondrocytes from bovine cartilaginous tissues

Cited by 4 publications

References 32 publications

Nanoencapsulation of Stem Cells within Polyelectrolyte Multilayer Shells

Nanoencapsulation of Stem Cells within Polyelectrolyte Multilayer Shells

An arthritogenic monoclonal antibody to type II collagen, CII‐C1, impairs cartilage formation by cultured chondrocytes

Directing Stem Cell Differentiation into the Chondrogenic Lineage In Vitro

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