Temporal changes in superoxide dismutase, catalase, and heat shock protein 70 gene expression, cortisol and antioxidant enzymes activity of Labeo rohita fingerlings subjected to starvation and refeeding

Dar, Showkat Ahmad; Srivastava, Prem Prakash; Varghese, Tincy; Nazir, Mir Ishfaq; Gupta, Subodh; Krishna, Gopal

doi:10.1016/j.gene.2018.12.058

Cited by 39 publications

(24 citation statements)

References 34 publications

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“…It has been proved that the most adverse influence of food deprivation on fish health could be associated with the generation of ROS due to the depletion of antioxidant reserves in the body (Morales et al, 2004). In this context, Dar et al (2019) reported that SOD and CAT activities pronouncedly increased in the liver and gills of rohu (Labeo rohita) subjected to one week of FA that was associated with overexpression of antioxidant related genes, but antioxidant activities and their related genes remarkably reduced in the tissues after one week RF period. In the present study, the values of TBARs were pronouncedly increased in the liver of T2 and T3 groups in both species suggesting a longer RF period is required for recuperation of oxidative stress in both groups.…”

Section: Discussionmentioning

confidence: 99%

Effects of a single-phase fasting period and subsequent re-feeding on compensatory growth, digestive enzyme activities, and antioxidant capacity of sobaity (Sparidentex hasta) and yellowfin seabream (Acanthopagrus latus)

Mozanzadeh

Safari

Ghaedi

et al. 2022

Annals of Animal Science

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An eight-week research was carried out to examine the influence of fasting (FA) and refeeding (RF) episodes on the compensatory growth responses (CGR) in sobaity (Sparidentex hasta, 10 g) and yellowfin seabreams (Acanthopagrus latus, 4.3 g) juveniles. Fish were fed with a commercial feed (contained 500 g kg−1 crude protein and 150 g kg−1 crude lipid) as following regimes: control (C, fish were fed three times every day), T1 (two weeks of feeding, one week of FA, and five weeks of RF), T2 (one week of feeding, two weeks of FA and five weeks of RF) and T3 (three weeks of FA and five weeks of RF). Two hundred and forty S. hasta juveniles were stocked into twelve 300-L tanks (20 fish tank−1), and 360 A. latus juveniles were allocated into other 12 tanks (30 fish tank−1). Each treatment was carried out in triplicates for each species, and each tank held only one of the species. The experiment was carried out for both species simultaneously. The weight and length of fish from the four groups were measured individually after the third week (after FA episode) and after eight weeks (after RF episode). After finishing the RF episode (eighth week), six fish of each tank were sacrificed with an overdose of 2-phenoxyethanol (1000 mg L−1), and the liver and the whole gut of the sacrificed fish were sampled, dissected, and then kept in a freezer (−80 °C) until further analyses. Survival rate was decreased in S. hasta juveniles with increasing the FA period mainly due to their cannibalistic behavior, which was triggered by starvation, but it was not affected in A. latus. The fasted groups in both species were significantly lost their weight after FA episodes. After five weeks of RF, S. hasta showed full compensatory growth response; meanwhile A. latus had a partial compensatory response (P<0.05). Hepatosomatic index value decreased after the FA period in both species, but it was restored to the normal level after RF phase. The activities of liver catalase, superoxide dismutase, glutathione-S-transferase, and glutathione peroxidase were increased in T2 group in S. hasta, but liver antioxidant enzymes were not affected in A. latus. In both species, the amount of the lipid peroxidation was significantly increased in the liver of fish groups subjected to T2 or T3 compared to T1 and control groups (P < 0.05). Liver alanine aminotransferase (ALT) and alkaline phosphatase (ALP) in S. hasta fasted for two weeks were higher than the other groups. The activities of trypsin, chymotrypsin, α-amylase, and lipase in S. hasta fasted for a week (T1) were higher than control. In addition, the activities of trypsin and chymotrypsin in A. latus fasted for two or three weeks were lower than C and T1 groups. The activity of ALP was increased with increasing FA period in both species. The findings of the present study showed that single-phase FA episodes reduce survival and induce oxidative stress in S. hasta juveniles; meanwhile A. latus juveniles did not show complete compensatory growth after RF episode.

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Section: Discussionmentioning

confidence: 99%

Effects of a single-phase fasting period and subsequent re-feeding on compensatory growth, digestive enzyme activities, and antioxidant capacity of sobaity (Sparidentex hasta) and yellowfin seabream (Acanthopagrus latus)

Mozanzadeh

Safari

Ghaedi

et al. 2022

Annals of Animal Science

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“…Also, Ashouri et al (2020) reported that after the fasting phase, the levels of plasma AST, ALP and LDH were increased in juvenile Siberian sturgeon ( Acipenserbaerii , Brandt 1869) starved for 1, 2 or 3 weeks compared with the fish that were fed throughout the husbandry trial. Dar et al (2019) also reported that the serum AST and ALT levels were increased during starvation in Labeo rohita , but they were recovered to the values of the control group during refeeding phase. In contrast, Azodi et al (2015) reported that plasma alkaline phosphatase and aspartate aminotransferase did not affect by cyclical fasting and refeeding periods in rainbow trout ( Oncorhynchus mykiss ).…”

Section: Discussionmentioning

confidence: 94%

“…In addition, the elevation of the antioxidant enzyme activities in both sparids may be related to the increased hydrogen peroxides produced by the neutralization of ROS generated through lipid peroxidation during restricted‐ration period (Najafi et al, 2014). In this regard, Dar et al (2019) reported that starvation boosted up the activities of SOD and CAT in Indian major carp ( Labeo rohita ). Similar to these results, Tamadoni et al (2020) also reported that increasing fasting duration elevated CAT and GST activities in the liver and resulted in oxidative stress in A .…”

Section: Discussionmentioning

confidence: 99%

Compensatory growth, antioxidant capacity and digestive enzyme activities of Sobaity ( Sparidentex hasta ) and yellowfin seabreams ( Acanthopagrus latus ) subjected to ration restriction

et al. 2021

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The compensatory growth response (CGR) and some selected physiological parameters were evaluated in sobaity (Sparidentex hasta, 10 g) and yellowfin seabreams (Acanthopagrus latus, 4.3 g) juveniles subjected to a 2‐week restricted feeding. Fish were first fed at 0%, 25%, 50% and 75% of the satiation level for a 2‐week period, and then, they were refed for 6 weeks at the visual satiation level. The control group was fed to the satiation everyday for 8 weeks. Three hundred sobaty seabream juveniles were stocked into 15 tanks (20 fish tank−1), and 450 yellowfin seabream juveniles were allocated into 15 tanks (30 fish tank−1) and were fed on a commercial feed (500 g kg−1 crude protein and 150 g kg−1 crude fat). Survival rate was decreased in R0% group in S. hasta because of the cannibalism, which was triggered by feed restriction. After the 2 weeks of feed restriction, the control and R0% treatments in S. hasta (16.0 vs. 9.3 g) and A. latus (3.9 vs. 6.2 g) had the highest and lowest body weight, respectively. After the 6 weeks of refeeding trial, all the ration‐restricted groups showed a full CGR in S. hasta. Regarding A. latus juveniles, except for R0%, the other groups showed a full CGR. Feed conversion ratio was improved in S. hasta that subjected to the 2‐week feed restriction, but this parameter did not change in A. latus. Antioxidant enzyme activities in the liver of S. hasta juveniles, including glutathione‐S‐transferase, glutathione peroxidase, superoxide dismutase and catalase along with liver enzymes including alkaline aminotransferase, aspartate amino transferase, lactate dehydrogenase and alkaline phosphatase gradually were decreased with reducing the severity of the feed restriction. Regarding A. latus, all mentioned antioxidant and the liver enzymes in the feed‐restricted groups were higher than the control. R75% and control groups in S. hasta had the greatest and the least trypsin and alkaline phosphatase activities. In A. latus, R25%, R50% and R75% groups had higher trypsin, chymotrypsin and lipase activities compared with the other groups (p < .05). By considering all the selected physiological responses in both species, it can be concluded that increasing the feed restriction severity can trigger oxidative stress that may compromise health condition.

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“…Since hspb1 peaks at 15 dph when yolk-reserves are depleted, this gene might be induced in response to stress as induced by food deprivation. Heat-shock proteins are induced by food deprivation in other fish species [122,123]. The increase of these genes during early ontogeny might reflect the overall increase in activity at the start of active swimming (8 dph) and feed searching behavior (15 dph).…”

Section: Activity: Movement and Stressmentioning

confidence: 99%

What Goes Wrong during Early Development of Artificially Reproduced European Eel Anguilla anguilla? Clues from the Larval Transcriptome and Gene Expression Patterns

Jéhannet

Palstra

Heinsbroek³

et al. 2021

Animals

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In eels, large variations in larval mortality exist, which would impede the viable production of juvenile glass eels in captivity. The transcriptome of European eel larvae was investigated to identify physiological pathways and genes that show differential regulation between non-viable vs. viable larvae. Expression of genes involved in inflammation and host protection was higher, suggesting that non-viable larvae suffered from microbial infection. Expression of genes involved in osmoregulation was also higher, implying that non-viable larvae tried to maintain homeostasis by strong osmoregulatory adaptation. Expression of genes involved in myogenesis, neural, and sensory development was reduced in the non-viable larvae. Expression of the major histocompatibility complex class-I (mhc1) gene, M-protein (myom2), the dopamine 2B receptor (d2br), the melatonin receptor (mtr1), and heat-shock protein beta-1 (hspb1) showed strong differential regulation and was therefore studied in 1, 8, and 15 days post-hatch (dph) larvae by RT-PCR to comprehend the roles of these genes during ontogeny. Expression patterning of these genes indicated the start of active swimming (8 dph) and feed searching behavior (15 dph) and confirmed immunocompetence immediately after hatching. This study revealed useful insights for improving larval survival by microbial control and salinity reduction.

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Temporal changes in superoxide dismutase, catalase, and heat shock protein 70 gene expression, cortisol and antioxidant enzymes activity of Labeo rohita fingerlings subjected to starvation and refeeding

Cited by 39 publications

References 34 publications

Effects of a single-phase fasting period and subsequent re-feeding on compensatory growth, digestive enzyme activities, and antioxidant capacity of sobaity (Sparidentex hasta) and yellowfin seabream (Acanthopagrus latus)

Effects of a single-phase fasting period and subsequent re-feeding on compensatory growth, digestive enzyme activities, and antioxidant capacity of sobaity (Sparidentex hasta) and yellowfin seabream (Acanthopagrus latus)

Compensatory growth, antioxidant capacity and digestive enzyme activities of Sobaity ( Sparidentex hasta ) and yellowfin seabreams ( Acanthopagrus latus ) subjected to ration restriction

What Goes Wrong during Early Development of Artificially Reproduced European Eel Anguilla anguilla? Clues from the Larval Transcriptome and Gene Expression Patterns

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