Temporal distinctions in the synthesis and accumulation of proteins by oocytes and cumulus cells during maturation in vitro of bovine oocytes

Wu, Bin; Ignotz, George G.; Currie, W. B.; Yang, Xiangzhong

doi:10.1002/(sici)1098-2795(199612)45:4<560::aid-mrd18>3.0.co;2-#

Cited by 32 publications

(35 citation statements)

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“…During the final stages of maturation from GV to M-II, mouse oocytes undergo pronounced morphologic and biochemical changes that include the synthesis of new cytoplasmic proteins [28], protein phosphorylation [29], and intracellular Ca 2ϩ oscillations [30]; similar changes have also been reported for other mammalian species, including humans [31][32][33]. The newly synthesized proteins appear to be necessary for the oocyte to complete normal meiosis to M-II, and they may be involved in development of the PN after activation (mouse [34] and other mammals [35][36][37]).…”

Section: Discussionmentioning

confidence: 93%

“…Comparative studies of protein synthesis between in vitroand in vivo-matured oocytes have revealed that several proteins with distinct molecular weights are present only in the cytoplasm of oocytes that matured in vivo (mouse [28] and other mammals [38,39]). Studies in the rabbit and cow suggest that some of these proteins may depend on cumulus cell-oocyte interactions since more proteins are present in the cytoplasm when oocytes are cultured within an intact cumulus mass [33,40,41]. However, because mouse oocytes can complete meiosis and activation even when denuded of cumulus cells at the GV stage [6,12,24], the proteins important for these processes would appear to occur independently of cumulus cell contact.…”

Section: Discussionmentioning

confidence: 99%

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Ooplasmic Influence on Nuclear Function During the Metaphase II-Interphase Transition in Mouse Oocytes

Liu

Krey

Zhang

et al. 2001

Biology of Reproduction

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Nuclear and pronuclear transfer procedures were used to assess the functional competence of the nucleus and cytoplasm of mouse germinal vesicle-stage oocytes denuded of granulosa cells and matured in vitro or in vivo before artificial activation using a sequential treatment of A23187 + cycloheximide. Following activation, in vitro-matured oocytes were "fertilized" by inserting a male pronucleus (PN), cultured to the 2-cell stage, and then transferred to the oviducts of foster mothers. No live births were noted, whereas a 17% live birth rate was observed when in vivo-matured oocytes were used. The developmental competency of other zygotes was similarly assessed following the exchange of haploid PN of matured and activated eggs with the female PN of fertilized zygotes. When PN of oocytes subjected to maturation and activation in vitro were transferred, only 1 of 79 reconstructed zygotes developed to term. In contrast, the live birth rate was 21% (11 of 53) for zygotes reconstructed with PN from in vivo-matured oocytes. Moreover, a live birth rate of 23% (8 of 35) was observed for reconstructed zygotes with female PN from "hybrid" oocytes created by transferring the metaphase II nuclei of in vitro-matured oocytes into enucleated, in vivo-matured oocytes before activation. Such results suggest that the nucleus of an in vitro-matured oocyte can support embryonic development, but only when it is activated in the proper ooplasmic milieu. The cellular factors creating this ooplasmic milieu appear to develop normally in vivo during follicle maturation to metaphase II, but they fail to do so when the oocytes are denuded of granulosa cells and cultured in vitro before the final stages of maturation. In parallel studies, male and female PN of in vivo-fertilized zygotes were inserted into oocytes that were activated and enucleated following either in vitro or in vivo maturation. Live birth rates were comparable at 19% (5 of 27) and 18% (9 of 49), respectively, suggesting that, regardless of the environment of the final stages of oocyte maturation, the resultant ooplasm is competent to support all aspects of embryonic development once activation and PN formation has been completed. Such findings only point further toward the importance of the condition of the ooplasmic milieu at the time of chemical activation. Whether a similar situation exists when eggs are activated following sperm penetration remains to be determined.

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Section: Discussionmentioning

confidence: 93%

Section: Discussionmentioning

confidence: 99%

Ooplasmic Influence on Nuclear Function During the Metaphase II-Interphase Transition in Mouse Oocytes

Liu

Krey

Zhang

et al. 2001

Biology of Reproduction

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“…During maturation, the oocyte nucleus progress from germinal vesicle to metaphase II stage. This process occurs in a time-dependent manner, and the following steps are observed: GVBD, M I, A/T, first polar body (first PB) emission, and M II (Wu et al 1996;Stock et al 1997).…”

Section: Discussionmentioning

confidence: 99%

The effects of macromolecular and serum supplements and oxygen tension during bovine in vitro procedures on kinetics of oocyte maturation and embryo development

Mingoti

Castro

Méo

et al. 2011

In Vitro Cell.Dev.Biol.-Animal

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Aiming to standardize in vitro production of bovine embryos and to obtain supplements to replace serum in culture media, this study evaluated the nuclear maturation kinetics and embryonic development in bovine after in vitro maturation (IVM) and culture (IVC) with several macromolecules (animal origin: bovine serum albumin (BSA), fetal calf serum (FCS); synthetic: polyvinyl alcohol (PVA), polyvinyl pyrrolidone (PVP), Ficoll, and Knockout) at two oxygen tensions (20% and 5% O(2)). Regarding nuclear kinetics, neither the presence of the expected stage (metaphase I, transition anaphase to telophase, and metaphase II) at each evaluation moment (6, 18, and 24 h after IVM, respectively) nor the accelerated polar body emission (at 18 h after IVM) related developmental competence to blastocyst stage when different supplements were compared. Independently of supplement, cleavage rates at 20% O(2) (61.6-79.2%) were higher than at 5% O(2) (38.9-58.7%). At 20% O(2), higher blastocyst and hatching rates, respectively, were obtained in treatments BSA, FCS, Knockout, and control group (IVM with FCS and IVC with BSA + FCS, 14.0-23.5% and 6.8-15.4%) in comparison to PVA, PVP, and Ficoll (0%). The same was observed at 5% O(2) for blastocyst rates with BSA, FCS, Knockout, and control (5.4-16.8%) and for hatching rates with BSA, FCS, and control (2.0-11.1%). We can conclude that producing bovine embryos at 20% O(2) during the entire IVP process resulted in higher developmental rates than at 5% O(2). In addition, while defined macromolecules PVA, PVP, and Ficoll were not suitable for embryonic development, the synthetic serum Knockout was able to replace serum and albumin for IVP in bovine at 20% O(2).

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“…2004). Protein synthesis in bovine oocytes increases during the first 12 h of in vitro culture (Wu et al. 1996), approximately at the time of the germinal vesicle’s breakdown (Tomek et al.…”

Section: Introductionmentioning

confidence: 99%

Influence of the Cumulus and Gonadotropins on the Metabolic Profile of Porcine Cumulus–Oocyte Complexes During In Vitro Maturation

Álvarez

Dalvit

Cetica

2012

Reprod Domestic Animals

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The aim of this work was to examine the influence of the cumulus and gonadotropins on the metabolic profile of porcine cumulus oocyte complexes (COCs) during in vitro maturation. Immature COCs were assigned to morphological classes A(1) (with a dense cumulus), A(2) (with a translucent cumulus), B(1) (with the corona radiata), B(2) (with only some remaining cumulus cells) and matured with or without gonadotropins. Glycolysis and ammonia production were higher in the A class COCs; gonadotropins increased both, especially in the A(1) COCs (p < 0.05). The A class COCs had the highest initial protein contents and at the end of in vitro maturation. Furthermore, hormonal stimulation induced a similar increase in protein contents of both A classes (p < 0.05). The neutral lipid content and reactive oxygen species (ROS) levels were similar in the immature oocytes of the COCs of all classes. A reduction was seen in both these variables when maturation proceeded either in the presence or absence of gonadotropins. The cumulus type surrounding the oocyte is related to the metabolism of carbohydrates and amino acids by the COC during in vitro maturation under gonadotropic stimulation. Oocyte lipolytic activity and ROS production appear to be independent of the surrounding cumulus and the presence of gonadotropins.

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Temporal distinctions in the synthesis and accumulation of proteins by oocytes and cumulus cells during maturation in vitro of bovine oocytes

Cited by 32 publications

References 40 publications

Ooplasmic Influence on Nuclear Function During the Metaphase II-Interphase Transition in Mouse Oocytes

Ooplasmic Influence on Nuclear Function During the Metaphase II-Interphase Transition in Mouse Oocytes

The effects of macromolecular and serum supplements and oxygen tension during bovine in vitro procedures on kinetics of oocyte maturation and embryo development

Influence of the Cumulus and Gonadotropins on the Metabolic Profile of Porcine Cumulus–Oocyte Complexes During In Vitro Maturation

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