Temporal distribution of dengue virus serotypes in Colombian endemic area and dengue incidence: re-introduction of dengue-3 associated to mild febrile illness and primary infection

Ocazionez, Raquel Elvira; Cortés, Fabián; Villar, Luis; Gómez, Sergio Yebrail

doi:10.1590/s0074-02762006000700004

Cited by 45 publications

(45 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…We compared viral genomes amplified directly from serum samples or from insect cells infected with viruses (Aquino et al 2006) andRJ (Nogueira et al 2005), and this genotype is the only one to have been implicated in DENV-3 epidemics in the Americas (Usuku et al 2001, Messer et al 2003, Uzcategui et al 2003, Peyrefitte et al 2005, Rigau-Pérez & Laufer 2006, Ocazionez et al 2006. When samples received from Porto Velho were used to infect C6/36 cells, they displayed a cytotoxic effect not seen with other DENV-3 viral isolates (data not shown).…”

Section: Resultsmentioning

confidence: 99%

Evidence for the co-circulation of dengue virus type 3 genotypes III and V in the Northern region of Brazil during the 2002-2004 epidemics

Nogueira

Stella²,

Bordignon³

et al. 2008

Mem. Inst. Oswaldo Cruz

View full text Add to dashboard Cite

The reintroduction of dengue virus type 3 (DENV-3) in Brazil in 2000 and its subsequent spread throughout the country was associated with genotype III viruses, the only DENV-3 genotype isolated in Brazil prior to 2002. We report here the co-circulation of two different DENV-3 genotypes in patients living in the Northern region of Brazil during the 2002-2004 epidemics. Complete genomic sequences of viral RNA were determined from these epidemics, and viruses belonging to genotypes V (Southeast Asia/South Pacific) and III were identified. This recent co-circulation of different DENV-3 genotypes in South America may have implications for pathological and epidemiological dynamics.Key words: dengue virus type 3 -genotype III and V -co-circulation -phylogenetic analysis -AmazonDengue is an emerging infectious disease affecting almost 50 million people in tropical and subtropical regions of the world. Dengue fever (DF) and dengue hemorrhagic fever (DHF) are caused by four closely related, but antigenically different dengue virus serotypes 1-4 (DENV-1-4) (Gubler & Clark 1995). Since the introduction of the DENV into Brazil in 1986, more than four million cases of dengue have been reported (Cordeiro et al. 2007), and the incidence of severe clinical symptoms has increased. No vaccine or specific therapeutic antiviral measures are available.Several studies have suggested that individuals experiencing a second dengue infection with a heterologous serotype are at greater risk for developing DHF or dengue shock syndrome (DSS) (Cummings et al. 2005, Halstead et al. 2005. However, DHF and DSS are also observed in primary cases, and not all secondary infections correspond to severe forms of the disease. Studies based on molecular epidemiology have provided evidence that differences in virulence between viral strains could play a role in the severity of the disease (Mangada & Igarashi 1998, Rico-Hesse 2003.Major dengue virus epidemics occurred in Brazil in 1986 and 1990, due to the introduction of DENV-1 and -2, respectively, and the subsequent spread of these serotypes throughout the country. DENV-3 reappeared in 1994 in Central America (Nicaragua and Panama) after 17 years of absence, and later spread to Mexico and the Caribbean (CDC 1995, Guzman et al. 1996, Usuku et al. 2001. In 2000, the presence of this serotype was re- PATIENTS, MATERIALS AND METHODSClinical samples and viruses -A serum sample from a dengue positive patient who had traveled to an area of epidemic dengue in RJ in Southeastern Brazil, and eight serum samples from patients living in Acre (AC) and RO in Northern Brazil (Table I, Fig. 1) were kindly provided by LACEN, Curitiba and IPEPATRO, Porto Velho, respectively. All patients were diagnosed with DF. These samples were used for the characterization of DENV genomes.RNA extraction and RT-PCR -Viral RNA was isolated from 140 μl of patient serum samples, taken during the acute phase of the disease and/or from 140 μl of purified virus prepared from cell culture supernatants after three passages in C6/36 cells, ...

show abstract

Section: Resultsmentioning

confidence: 99%

Evidence for the co-circulation of dengue virus type 3 genotypes III and V in the Northern region of Brazil during the 2002-2004 epidemics

Nogueira

Stella²,

Bordignon³

et al. 2008

Mem. Inst. Oswaldo Cruz

View full text Add to dashboard Cite

show abstract

“…24 By using a balanced sample of isolates from different countries, our analysis also provided a comprehensive view of the dispersion of DENV-3 in South America ( Figure 3 ) passed to Colombia where it was detected in 2001 in the eastern province of Santander but does not seems to have extended to other countries. 29 The Ecuadorian lineage, first detected in 1999 in that country, was isolated in Peru in 2000 17 and in Colombia in the southern province of Putumayo in 2002. 27 Although other studies did not find overlaps in the areas of dispersion of the three lineages, we show here that two of them have met in Medellín, at least since 2003.…”

Section: Discussionmentioning

confidence: 99%

“…28 The DENV-3 was isolated again in 2001 in the eastern province of Santander. 29 It then spread to other provinces in the following years, including Antioquia and its capital, Medellín, where DENV has been regularly diagnosed since 1992 with peak incidence in 1998, 30 DENV-3 is highly prevalent now in Medellín and in other parts of Colombia and other South American countries. 8 In an effort to understand the origin and evolution of DENV-3 circulating in Medellín and any possible relation to the severity or epidemic behavior of the disease, a phylogenetic analysis was conducted of DENV-3 isolated from clinical cases between 2002 and 2007 in the area.…”

Section: Introductionmentioning

confidence: 99%

Prolonged Co-circulation of Two Distinct Dengue Virus Type 3 Lineages in the Hyperendemic Area of Medellín, Colombia

Ospina¹,

Díaz²,

Osorio³

2010

The American Society of Tropical Medicine and Hygiene

View full text Add to dashboard Cite

During the past two decades, Dengue virus-3 (DENV-3) has re-emerged in the Western Hemisphere causing significant epidemics of dengue fever (DF) and dengue hemorrhagic fever (DHF). In an effort to understand the molecular evolution of DENV-3 and their relationships to other DENV-3 circulating in the western hemisphere, we conducted a phylogenetic study on DENV-3 isolates made between 2002 and 2007 in the metropolitan area of Medellín, Colombia. An unexpected co-circulation of two different variants of DENV-3 subtype III during at least 5 years in Medellín was found. In addition, a more complete analysis of DENV-3 viruses isolated in other South American regions revealed the existence of three different subtype III lineages, all derived from independent introductions. This study documents significant genetic diversity of circulating viruses within the same subtype and an unusual capacity of the population of this city to support continuous circulation of multiple variants of dengue virus.

show abstract

“…6 The DENV-3 subsequently expanded to Central American countries and Mexico in 1995, Puerto Rico in 1998, and other Caribbean islands, causing major epidemics in several Central American countries, Mexico, 108,109 and later to South America. [110][111][112] In 1996, PAHO approved a Continental Plan to intensify Ae. aegypti control; however, it was not fully implemented.…”

Section: Aedes Aegypti Reinfestation (1971-1999)mentioning

confidence: 99%

The History of Dengue Outbreaks in the Americas

Dick

Martín²,

Montoya³

et al. 2012

The American Society of Tropical Medicine and Hygiene

370

264

View full text Add to dashboard Cite

Dengue is a viral disease usually transmitted by Aedes aegypti mosquitoes. Dengue outbreaks in the Americas reported in medical literature and to the Pan American Health Organization are described. The outbreak history from 1600 to 2010 was categorized into four phases: Introduction of dengue in the Americas (1600–1946); Continental plan for the eradication of the Ae. aegypti (1947–1970) marked by a successful eradication of the mosquito in 18 continental countries by 1962; Ae. aegypti reinfestation (1971–1999) caused by the failure of the mosquito eradication program; Increased dispersion of Ae. aegypti and dengue virus circulation (2000–2010) characterized by a marked increase in the number of outbreaks. During 2010 > 1.7 million dengue cases were reported, with 50,235 severe cases and 1,185 deaths. A dramatic increase in the number of outbreaks has been reported in recent years. Urgent global action is needed to avoid further disease spread.

show abstract

Temporal distribution of dengue virus serotypes in Colombian endemic area and dengue incidence: re-introduction of dengue-3 associated to mild febrile illness and primary infection

Cited by 45 publications

References 26 publications

Evidence for the co-circulation of dengue virus type 3 genotypes III and V in the Northern region of Brazil during the 2002-2004 epidemics

Evidence for the co-circulation of dengue virus type 3 genotypes III and V in the Northern region of Brazil during the 2002-2004 epidemics

Prolonged Co-circulation of Two Distinct Dengue Virus Type 3 Lineages in the Hyperendemic Area of Medellín, Colombia

The History of Dengue Outbreaks in the Americas

Contact Info

Product

Resources

About