Temporal distribution of human rotavirus serotypes 1, 2, 3, and 4 in venezuelan children with gastroenteritis during 1979–1989

White, Laura; Garcia, D; Boher, Y; Blanco, Mireya González; Pérez, Mireya; Römer, H; Flores, Jorge; Pérez‐Schael, Irene

doi:10.1002/jmv.1890340202

Cited by 26 publications

(17 citation statements)

References 21 publications

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“…Previous serological studies conducted in Venezuela have demonstrated temporal variation in the predominant circulating of RV G serotype, but in general G1, G3, or G4 and moderate rates of circulation of G2 strains have been detected [White et al, 1991;Pérez-Schael et al, 1999]. This study shows that G3P[8]/ NSP4-E1 was the most common RV genotype combination detected in strains circulating in Valencia, Venezuela, in 2003.…”

Section: Discussionmentioning

confidence: 56%

Genotyping of human rotaviruses circulating among children with diarrhea in Valencia, Venezuela

Vizzi

Piñeros

González

et al. 2011

Journal of Medical Virology

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Rotavirus infection is the most common cause of severe gastroenteritis during childhood worldwide, especially in developing countries. Two rotavirus vaccines are available for childhood immunization programs. Evaluation of the vaccine performance will benefit from knowledge of the epidemiological features of rotavirus infection in regional settings. Limited information on the molecular characteristics of the rotavirus types circulating in Venezuela is available. Eighty seven (89.7%) of the 97 ELISA rotavirus positive stool samples collected from children with diarrhea aged <5 years during 2003 in Valencia (Carabobo State), were G-, P- and NSP4-genotyped by RT-PCR and/or automated sequencing. Four common combinations, G3P[8]/NSP4-E1, G2P[4]/NSP4-E2, G9P[8]/NSP4-E1, and G1P[8]/NSP4-E1 were responsible for 50.6%, 35.6%, 5.7%, and 1.1%, respectively of cases of rotavirus diarrhea, most of them (66%) in children ≤12 months. One uncommon G8P[14]/NSP4-E2 strain was also detected. Temporal fluctuation of genotype distribution occurred, but no differences by age, diarrhea severity score, sex, treatment type or patient medical attention were observed, except for the G3P[8]/NSP4-E1, associated with a more severe dehydration than any other type (P < 0.01). The results confirm the broad diversity among rotavirus strains circulating in Venezuela prior to vaccine implementation, showing the predominance of G3, significant proportion of G2 and moderate circulation of G9 strains. Epidemiological surveillance is needed to detect the emergence of new genotypes that could escape protection induced by vaccination.

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Section: Discussionmentioning

confidence: 56%

Genotyping of human rotaviruses circulating among children with diarrhea in Valencia, Venezuela

Vizzi

Piñeros

González

et al. 2011

Journal of Medical Virology

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“…While variability in G serotypes from one rotavirus season to another is known and long-term surveys are required to correctly evaluate the relative frequencies of rotavirus G serotypes, there are a few studies showing that a serotype other than G1 was the most prevalent among long-term surveys performed over five years or more. Serotype G3 was predominant in India (32.5%) (3) and in Venezuela (24.6%) (73). Serotype G4 was the most prevalent in Malaysia (41.5%) (56) and Bangladesh (32.5%) (64).…”

Section: Discussionmentioning

confidence: 99%

The Relative Frequencies of G Serotypes of Rotaviruses Recovered from Hospitalized Children with Diarrhea: A 10‐Year Survey (1987–1996) in Japan with a Review of Globally Collected Data

Koshimura

Nakagomi

2000

Microbiology and Immunology

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Since rotavirus vaccines aim to protect children from severe diarrhea, knowledge of the prevailing G serotypes among rotaviruses from hospitalized children is essential. Thus, we determined the G serotypes of rotaviruses collected from children with acute diarrhea in a local referral hospital in Akita, Japan, over the 10-year period between January 1987 and December 1996. Based on the assumption that rotaviruses with an identical electropherotype possess the same G serotype, the G serotypes of 488 rotavirus-positive specimens that were classified into 63 electropherotypes were determined by enzyme-linked immunosorbent assay with a supplementary use of G typing by reverse transcription-PCR. The relative frequencies over the 10-year period were 77.0 (G1), 14.5 (G2), 2.7 (G3) and 5.3% (G4), leaving the possibility that only 0.4 % had G serotypes uncommon to human rotaviruses. Of 24,050 rotaviruses extracted by reviewing 63 serotyping studies in literature, the relative frequencies of the four major G serotypes were 50.6 (G1), 9.3 (G2), 7.2 (G3) and 11.6% (G4). As to uncommon G serotypes, only 0.9% were described as serotypes other than G1-4, and our estimate for potential uncommon serotypes were at most 8.1%. Thus, both this long-term study focusing on the rotaviruses only from severe cases in a single hospital in Japan and the global review of G serotypes published to date indicate that the primary target of any rotavirus vaccines should be rotaviruses possessing serotypes G1-4.

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“…Rotavirus-positive specimens were serotyped by monoclonal antibody ELISA as previously described [Flores et al, 1988;White et al, 1991]. The monoclonal antibodies used for serotyping were generously obtained from Drs.…”

Section: Rotavirus Antigen Detection and Serotypingmentioning

confidence: 99%

Epidemiological features of rotavirus infection in Caracas, Venezuela: Implications for rotavirus immunization programs

et al. 1999

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The epidemiological features of rotavirus infection may be quite relevant for evaluation of the performance of a rotavirus vaccine in different settings, as well as for monitoring its impact during vaccination under routine conditions. This article describes some important issues regarding rotavirus epidemiology in Venezuela, where major field trials of rotavirus vaccine have been carried out. Rotaviruses was significantly more frequently observed in inpatient (43%) than in outpatient (21%) consultations for diarrhea in infants and young children. There was a high prevalence of rotavirus illness, regardless of socioeconomic conditions, but the risk of dehydration was greater among the lower socioeconomic groups. Rotavirus disease occurs year-round, with a slight seasonal pattern. Eighty-five percent of rotavirus-positive diarrheal episodes, as well as 86% of cases of dehydration due to rotavirus, occurred during the first year of life. However, rotavirus illnesses occur less commonly during the first months of life (0-2 months), which may be a result of protection by transplacental antibodies. The pattern of acquisition of rotavirus antibody was consistent with this age distribution of disease and with optimal age for vaccination. Thus, regional epidemiological characteristics of rotavirus infection may affect optimal performance of rotavirus vaccine.

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Temporal distribution of human rotavirus serotypes 1, 2, 3, and 4 in venezuelan children with gastroenteritis during 1979–1989

Cited by 26 publications

References 21 publications

Genotyping of human rotaviruses circulating among children with diarrhea in Valencia, Venezuela

Genotyping of human rotaviruses circulating among children with diarrhea in Valencia, Venezuela

The Relative Frequencies of G Serotypes of Rotaviruses Recovered from Hospitalized Children with Diarrhea: A 10‐Year Survey (1987–1996) in Japan with a Review of Globally Collected Data

Epidemiological features of rotavirus infection in Caracas, Venezuela: Implications for rotavirus immunization programs

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