Temporal dynamic of biofilms enhances the settlement of the central-Mediterranean reef-builder Dendropoma cristatum (Biondi, 1859)

Marca, Emanuela Claudia La; Catania, Valentina; Tagliavia, Marcello; Mannino, Anna Maria; Chemello, Renato; Quatrini, Paola

doi:10.1016/j.marenvres.2021.105484

Cited by 3 publications

(1 citation statement)

References 55 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The structure of microbial communities was investigated by the bacterial and fungal automated ribosomal intergenic spacer analysis (B-ARISA and F-ARISA, respectively), using the bacterial primers ITSF/ITSReub [34], and the fungal primers 2234C/3126T [35] (Table S1). The forward primer (2234C) was modified including a degenerated position [36], improving the sensitivity of analyses and aiming to limit biases. The PCR fragments were labeled as described in Schuelke [37], using a sequence-specific forward primer with M13 tail at its 5 end (TGT AAA ACG ACG GCC AGT), a sequence-specific reverse primer not modified, and the universal fluorescent-labeled M13 primer (FAM-TGT AAA ACG ACG GCC AGT-3 ).…”

Section: Automated Ribosomal Intergenic Spacer Analysis (Arisa)mentioning

confidence: 99%

The Root Mycobiota of Betula aetnensis Raf., an Endemic Tree Species Colonizing the Lavas of Mt. Etna (Italy)

Badalamenti

Catania

Sofia

et al. 2021

Forests

Self Cite

View full text Add to dashboard Cite

Betula aetnensis is an endemic tree of high conservation value, which thrives on the nutrient-poor volcanic soils of Mount Etna. Since plant–microbe interactions could play a crucial role in plant growth, resource uptake, and resistance to abiotic stresses, we aimed to characterize the root and rhizosphere microbial communities. Individuals from natural habitat (NAT) and forest nursery (NURS) were surveyed through microscopy observations and molecular tools: bacterial and fungal automated ribosomal intergenic spacer analysis (ARISA), fungal denaturing gradient gel electrophoresis (DGGE). B. aetnensis was found to be simultaneously colonized by arbuscular (AM), ectomycorrhizal (ECM), ericoid (ERM) fungi, and dark septate endophytes (DSE). A high diversity of the bacterial community was observed whilst the root fungal assemblage of NAT plants was richer than that of NURS. Root and rhizosphere fungal communities from NAT plants were characterized by Illumina MiSeq sequencing. Most of the identified sequences were affiliated to Helotiales, Pezizales, and Malasseziales. Ascomycota and Basidiomycota dominated roots and rhizosphere but differed in community structure and composition. ECM in the roots mainly belonged to Tylospora and Leccinum, while Rhizopogon was abundant in the rhizosphere. The Helotiales, including ERM (mostly Oidiodendron) and DSE (mostly Phialocephala), appeared the dominant component of the fungal community. B. aetnensis harbors an extraordinarily wide array of root-associated soil microorganisms, which are likely to be involved in the adaptation and resistance mechanisms to the extreme environmental conditions in volcano Etna. We argue that nursery-produced seedlings could lack the necessary microbiota for growth and development in natural conditions.

show abstract

Section: Automated Ribosomal Intergenic Spacer Analysis (Arisa)mentioning

confidence: 99%