Temporal dynamics and depth variations of dissolved free amino acids and polyamines in coastal seawater determined by high-performance liquid chromatography

Lü, Xinxin; Long, Zhilin; Clevinger, Curtis C.; Liu, Qian; Hollibaugh, James T.; Mou, Xiaozhen

doi:10.1016/j.marchem.2014.04.004

Cited by 42 publications

(72 citation statements)

References 45 publications

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“…Our LC‐MS method allowed direct detection of sub‐nM concentrations of underivatized DFPA in diverse freshwater habitats at threshold concentrations that were comparable to or lower than previously reported ones (Nishibori , 2003; Lu et al ; Liu et al ; Lu et al ). The LOD of our method ranged between 0.05 nM and 0.5 nM (Supporting Information Table 2), which is sufficiently sensitive for the accurate quantification of compounds at low to sub‐nM levels in freshwater samples (Horňák and Pernthaler ; Horňák et al ).…”

Section: Discussionsupporting

confidence: 77%

Spatiotemporal distribution and microbial assimilation of polyamines in a mesotrophic lake

Krempaska

Horňák

2017

Limnology & Oceanography

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We examined seasonal and spatial variations of dissolved free polyamines (DFPA) in the large mesotrophic prealpine Lake Zurich (Switzerland). An ion‐pairing liquid chromatography method with mass spectrometric detection was optimized for the quantification of DFPA without prior concentration or derivatization. Total DFPA concentrations varied between 0.4 nM and 11 nM in winter and spring of 2015, respectively. Polyamine concentrations were highest in the epilimnion during the phytoplankton growth phase, and reflected occurrence patterns of diatoms. Putrescine consistently dominated the DFPA pool throughout the season whereas norspermidine was detected only once in spring. Incubations with 13C‐labeled putrescine revealed concentration‐dependent uptake rates (3.2–5 nM h−1) and short turnover times (2.4–4.4 h). In contrast, incorporation rates into biomass measured with 14C‐labeled putrescine were substantially lower (0.9 nM h−1), indicating that this compound was predominantly respired. Uptake kinetics moreover suggested that putrescine in situ uptake rates were limited by low ambient concentrations. Competition assays revealed that putrescine uptake could only be inhibited by excess concentrations of another polyamine (spermidine), but not by amino acids. Bacteria affiliated with Limnohabitans and the acI lineage of Actinobacteria were identified as the taxa with the highest fraction (25–32%) of putrescine‐incorporating cells. This compound was also utilized by Alphaproteobacteria, Cyclobacteriaceae, diatoms, and cyanobacteria. Overall, our data point to fast turnover of putrescine by different microorganisms, implying the importance of this substrate as an attractive energy source.

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Section: Discussionsupporting

confidence: 77%

Spatiotemporal distribution and microbial assimilation of polyamines in a mesotrophic lake

Krempaska

Horňák

2017

Limnology & Oceanography

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“…The dinoflagellate Amphidinium carterae contained the highest concentration of extractable PA (1000s of µmol l −1 ) among the phytoplankton species that we studied, indicating the potential of dinoflagellates to contribute significantly to the dissolved PA pool of the ocean. The PAs we found in these species included most of the PAs commonly detected in the ocean (Nishibori et al 2001a,b, 2003, Nishibori & Nishijima 2004, Lu et al 2014, Liu et al 2015, suggesting that variability of PA concentrations in seawater could be a consequence of varying composition in phytoplankton communities.…”

Section: Extractable Pa Profiles In Phytoplanktonmentioning

confidence: 70%

“…PA distributions in the ocean are usually correlated with phytoplankton biomass and bacterial production (Lee 1992, Lee & Jørgensen 1995, Lu et al 2014, Liu et al 2015. PUT and SPD are ubiquitous and relatively abundant (low nmol l −1 ), with other PAs present either at lower levels (e.g.…”

Section: Open Pen Access Ccessmentioning

confidence: 99%

Response of polyamine pools in marine phytoplankton to nutrient limitation and variation in temperature and salinity

Liu¹,

Nishibori²,

Imai³

et al. 2016

Mar. Ecol. Prog. Ser.

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“…Tau is a naturally occurring free non‐protein organic acid with a sulfonic group instead of the carboxyl group characteristic for amino acids. Only a few studies have reported dissolved free Tau concentrations in coastal and near‐shore surface waters, which are typically in the low nanomolar range (up to ∼35 nM) (Mopper and Lindroth ; Kuznetsova and Lee ; Lu et al ). However, to the best of our knowledge, dissolved free Tau concentrations have not been reported for open oceanic and deep waters.…”

mentioning

confidence: 99%

Crustacean zooplankton release copious amounts of dissolved organic matter as taurine in the ocean

Clifford

Hansell

Varela

et al. 2017

Limnology & Oceanography

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Taurine (Tau), an amino acid‐like compound, is present in almost all marine metazoans including crustacean zooplankton. It plays an important physiological role in these organisms and is released into the ambient water throughout their life cycle. However, limited information is available on the release rates by marine organisms, the concentrations and turnover of Tau in the ocean. We determined dissolved free Tau concentrations throughout the water column and its release by abundant crustacean mesozooplankton at two open ocean sites (Gulf of Alaska and North Atlantic). At both locations, the concentrations of dissolved free Tau were in the low nM range (up to 15.7 nM) in epipelagic waters, declining sharply in the mesopelagic to about 0.2 nM and remaining fairly stable throughout the bathypelagic waters. Pacific amphipod–copepod assemblages exhibited lower dissolved free Tau release rates per unit biomass (0.8 ± 0.4 μmol g−1 C‐biomass h−1) than Atlantic copepods (ranging between 1.3 ± 0.4 μmol g−1 C‐biomass h−1 and 9.5 ± 2.1 μmol g−1 C‐biomass h−1), in agreement with the well‐documented inverse relationship between biomass‐normalized excretion rates and body size. Our results indicate that crustacean zooplankton might contribute significantly to the dissolved organic matter flux in marine ecosystems via dissolved free Tau release. Based on the release rates and assuming steady state dissolved free Tau concentrations, turnover times of dissolved free Tau range from 0.05 d to 2.3 d in the upper water column and are therefore similar to those of dissolved free amino acids. This rapid turnover indicates that dissolved free Tau is efficiently consumed in oceanic waters, most likely by heterotrophic bacteria.

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Temporal dynamics and depth variations of dissolved free amino acids and polyamines in coastal seawater determined by high-performance liquid chromatography

Cited by 42 publications

References 45 publications

Spatiotemporal distribution and microbial assimilation of polyamines in a mesotrophic lake

Spatiotemporal distribution and microbial assimilation of polyamines in a mesotrophic lake

Response of polyamine pools in marine phytoplankton to nutrient limitation and variation in temperature and salinity

Crustacean zooplankton release copious amounts of dissolved organic matter as taurine in the ocean

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