Temporal dynamics of the response to Al stress in Eucalyptus grandis × Eucalyptus camaldulensis

Alcântara, Berenice Kussumoto de; Pizzaia, Daniel; Piotto, Fernando Ângelo; Borgo, Lucélia; Brondani, Gilvano Ebling; Azevedo, Ricardo Antunes

doi:10.1590/0001-3765201520140322

Cited by 11 publications

(6 citation statements)

References 46 publications

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“…Hence, it is suggested that this transcription factor is affected by seed priming and could be at least partially responsible for the increase in lignin gene expression in the roots. On the other hand, chelating Al in lignin would not result in a reduction of Al in the plant [ 23 , 64 ], therefore, it is implied that AsA is excluding Al in the sensitive line B73 because reduction of Al in roots was observed. In the work of Ezaki et al [ 23 ], wild-type and transgenic Arabidopsis thaliana lines treated with 100 μM Al showed morin-specific fluorescent signals (Al staining) in their root tips.…”

Section: Discussionmentioning

confidence: 99%

Dry Priming of Maize Seeds Reduces Aluminum Stress

et al. 2015

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Aluminum (Al) toxicity is directly related to acidic soils and substantially limits maize yield. Earlier studies using hormones and other substances to treat the seeds of various crops have been carried out with the aim of inducing tolerance to abiotic stress, especially chilling, drought and salinity. However, more studies regarding the effects of seed treatments on the induction of Al tolerance are necessary. In this study, two independent experiments were performed to determine the effect of ascorbic acid (AsA) seed treatment on the tolerance response of maize to acidic soil and Al stress. In the first experiment (greenhouse), the AsA seed treatment was tested in B73 (Al-sensitive genotype). This study demonstrates the potential of AsA for use as a pre-sowing seed treatment (seed priming) because this metabolite increased root and shoot growth under acidic and Al stress conditions. In the second test, the evidence from field experiments using an Al-sensitive genotype (Mo17) and an Al-tolerant genotype (DA) suggested that prior AsA seed treatment increased the growth of both genotypes. Enhanced productivity was observed for DA under Al stress after priming the seeds. Furthermore, the AsA treatment decreased the activity of oxidative stress-related enzymes in the DA genotype. In this study, remarkable effects using AsA seed treatment in maize were observed, demonstrating the potential future use of AsA in seed priming.

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Section: Discussionmentioning

confidence: 99%

Dry Priming of Maize Seeds Reduces Aluminum Stress

et al. 2015

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“…Some reports suggested that Eucalyptus has higher tolerance to Al toxicity than other tree species such as Quercus robur, Pinus radiata and Melaleuca cajuputi, and may even bene t from the presence of Al at some concentrations (Álvarez et al, 2005;Tahara et al, 2008;Huang and Bachelard, 1993). There were also differences in Al tolerance and response to Al stress among Eucalyptus species and their clones in acidic environments (Tahara et al, 2005a(Tahara et al, , 2008(Tahara et al, , 2014Alcântara et al, 2015;Barros et al, 2016;Sliva et al 2017). China is one of major producers of Eucalyptus with more than 4.5 million hectares under cultivation, accounting for about 2.1% of China's total forest area and 6.3% of its total plantation area.…”

Section: Introductionmentioning

confidence: 99%

Metabolic acclimation supports higher aluminium-induced secretion of citrate and malate in an aluminium-tolerant hybrid clone of Eucalyptus

Finnegan

Dai

et al. 2020

Preprint

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Background Eucalyptus is the main timber species, most of which are hybrid clones, and usually grow in aluminized acid soil in China. The exudation of organic acids from roots may contribute to detoxification of Al and lead to the Al-tolerance in Eucalyptus genotypes. To further understand the organic acid response in Al tolerance in Eucalyptus, the Al-tolerant Eucalyptus grandis × E. urophylla clone GL-9 (marked as “G9”) and the Al-sensitive Eucalyptus urophylla clone GL-4 (marked as “W4”) were used to investigate the secretion and metabolism of citrate and malate in roots. Results Eucalyptus seedlings in hydroponics were exposed to the presence or absence of 4.4 mM Al at pH 4.0 for 24 hours. The protein synthesis inhibitor cycloheximide (CHM) and the anion channel blocker phenylglyoxal (PG) were applied to explore possible pathways involved in organic acid secretion. The Al treatments caused higher Al accumulation in roots of both clones. The secretion of malate and citrate was greater in G9 than in W4, corresponding to the relatively higher tolerance in G9 to Al. The peak Al concentration occurred after 1 h in G9 roots and declined afterward, indicating the activation of detoxification to alleviate Al accumulation. After 6 h of Al exposure, the efflux of citrate dramatically increased in G9 after a substantial lag phase, while both peak Al accumulation in roots and peak malate secretion occurred and there was no induction of citrate secretion in W4. Enhanced activity for citrate synthase and phosphoenolpyruvate carboxylase, and reduced activity for NADP-isocitrate dehydrogenase, aconitase and NADP-malic enzyme were closely associated with the greater secretion of citrate in G9. Both PG and CHM were effective inhibitors of citrate and malate secretion in both Eucalyptus clones, except the malate secretion in W4 was not affected by CHM. Conclusions In two different Al-tolerant Eucalyptus clones, both secretion and internal accumulation of citrate and malate in roots were involved in Al detoxification. An anion channel on the plasma membrane could be an important mode of organic acid secretion. Citrate and relevant metabolizing enzymes led more important role in the response to Al in E. grandis × E. urophylla.

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“…Several studies have suggested that species of Eucalyptus have higher tolerance to Al toxicity than other tree species such as Quercus robur, Pinus radiata and Melaleuca cajuputi, and may even benefit from low concentrations of Al [10][11][12]. Moreover, species of Eucalyptus and their clones vary in their Al tolerance and response to Al in acidic environments [12][13][14][15][16][17]. As in other plants, the exudation of low molecular weight organic acids from the roots of several species or genotypes of Eucalyptus may be an important determinant for Al tolerance and may allow Eucalyptus to grow and yield well in acidic soils [13,18,19].…”

Section: Introductionmentioning

confidence: 99%

Metabolic acclimation supports higher aluminium-induced secretion of citrate and malate in an aluminium-tolerant hybrid clone of Eucalyptus

Finnegan

Dai

et al. 2021

BMC Plant Biol

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Background Eucalyptus is the main plantation wood species, mostly grown in aluminized acid soils. To understand the response of Eucalyptus clones to aluminum (Al) toxicity, the Al-tolerant Eucalyptus grandis × E. urophylla clone GL-9 (designated “G9”) and the Al-sensitive E. urophylla clone GL-4 (designated “W4”) were employed to investigate the production and secretion of citrate and malate by roots. Results Eucalyptus seedlings in hydroponics were exposed to the presence or absence of 4.4 mM Al at pH 4.0 for 24 h. The protein synthesis inhibitor cycloheximide (CHM) and anion channel blocker phenylglyoxal (PG) were applied to explore possible pathways involved in organic acid secretion. The secretion of malate and citrate was earlier and greater in G9 than in W4, corresponding to less Al accumulation in G9. The concentration of Al in G9 roots peaked after 1 h and decreased afterwards, corresponding with a rapid induction of malate secretion. A time-lag of about 6 h in citrate efflux in G9 was followed by robust secretion to support continuous Al-detoxification. Malate secretion alone may alleviate Al toxicity because the peaks of Al accumulation and malate secretion were simultaneous in W4, which did not secrete appreciable citrate. Enhanced activities of citrate synthase (CS) and phosphoenolpyruvate carboxylase (PEPC), and reduced activities of isocitrate dehydrogenase (IDH), aconitase (ACO) and malic enzyme (ME) were closely associated with the greater secretion of citrate in G9. PG effectively inhibited citrate and malate secretion in both Eucalyptus clones. CHM also inhibited malate and citrate secretion in G9, and citrate secretion in W4, but notably did not affect malate secretion in W4. Conclusions G9 immediately secrete malate from roots, which had an initial effect on Al-detoxification, followed by time-delayed citrate secretion. Pre-existing anion channel protein first contributed to malate secretion, while synthesis of carrier protein appeared to be needed for citrate excretion. The changes of organic acid concentrations in response to Al can be achieved by enhanced CS and PEPC activities, but was supported by changes in the activities of other enzymes involved in organic acid metabolism. The above information may help to further explore genes related to Al-tolerance in Eucalyptus.

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Temporal dynamics of the response to Al stress in Eucalyptus grandis × Eucalyptus camaldulensis

Cited by 11 publications

References 46 publications

Dry Priming of Maize Seeds Reduces Aluminum Stress

Dry Priming of Maize Seeds Reduces Aluminum Stress

Metabolic acclimation supports higher aluminium-induced secretion of citrate and malate in an aluminium-tolerant hybrid clone of Eucalyptus

Metabolic acclimation supports higher aluminium-induced secretion of citrate and malate in an aluminium-tolerant hybrid clone of Eucalyptus

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